FastQCFastQC Report
Fri 10 Feb 2017
ERR1631087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386218
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG136853.5433356291006635No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT104692.7106452832338213RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC75741.9610686192771958No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT50501.3075516936030946No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC37760.97768617723669RNA PCR Primer, Index 47 (95% over 23bp)
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC35250.9126969742477047No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG18850.4880663252360066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.4391302321486829No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16790.43472857298209816No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT10030.2596978908284958No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA9840.2547783894070188No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT9510.24623399220129566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6760.17503068215360237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC6300.033.7698441
GGTATCA2550.031.1960771
GATTACG303.5950923E-430.8333341
CGTCTTC4950.030.27272837
AGCAACG702.055458E-1029.071432
GCAACGC702.055458E-1029.071433
TTATACA7400.028.02
CCGTCTT16200.025.9228437
GCCGTCT16500.025.45151536
GATACAC451.320991E-424.6666663
GTATCAA8200.024.5914651
TATACAC9000.024.2555563
ATTACGG400.001928878223.1250022
GGGTACA400.001928878223.1250021
CTTTACG400.001928878223.12500235
ATACACA23950.022.0918581
TACACAT24150.022.062112
CACGGTA450.003821279220.5555552
TAGACTG450.003821279220.5555555
CGTGCGC450.003821279220.55555529
Produced by FastQC (version 0.11.5)