FastQCFastQC Report
Fri 10 Feb 2017
ERR1631086.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631086.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79854
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT890911.156610814736895TruSeq Adapter, Index 1 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG846910.605605229543917No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC45975.756756079845719No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC33924.247752147669496RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC31343.9246625090790697No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT16112.0174318130588325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14261.7857590101936032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12171.5240313572269393No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG10841.3574773962481528No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT7550.9454754927743132No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA6890.8628246549953665No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT6420.8039672402133895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5570.6975229794374734No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3450.43203847020812985No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT3440.43078618478723674No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2420.30305307185613745No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2220.27800736343827487No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1850.23167280286522904No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA1760.2204022340771909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.19410424023843514No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1510.18909509855486262No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1330.1665539609787863No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1310.164049390137No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC1310.164049390137No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG1250.15653567761164125No Hit
TCGCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT1180.14776967966538934TruSeq Adapter, Index 1 (95% over 21bp)
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG1170.1465173942444962No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG1150.14401282340270996No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG1120.14025596714003055No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG1100.13775139629824432No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1090.13649911087735117No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1050.13148996919377864No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.1302376837728855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC1030.12898539835199238No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT1010.12648082751020612No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA950.11896711498484734No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG930.11646254414306109No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG920.11521025872216796No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC880.11020111703859545No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC820.10268740451323664No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG800.1001828336714504No Hit
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA800.1001828336714504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC200.001831699537.03
TGATACA200.001831699537.02
TTTACAC403.7573045E-837.02
CTTATAC2450.035.48981
CGTCTTC4150.032.98795337
CTTTACA502.667548E-729.5999981
TATAAGA250.005466146429.59999813
AACTTTA250.005466146429.5999987
GTATAAG250.005466146429.59999812
TAACTTT250.005466146429.5999986
TTAACTT250.005466146429.5999985
TACTTTA250.005466146429.59999836
TTATACA2950.029.4745792
GGTATCA1100.028.5909081
TTCGCTC405.8722406E-527.7536
CGGGAGC405.8722406E-527.7516
TTACACA556.1399805E-726.909093
TCGCTCG358.7970204E-426.4285737
GTATCAA4100.026.1707321
CCGTCTT13200.025.22727237
Produced by FastQC (version 0.11.5)