##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631086.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79854
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.027800736343828	31.0	30.0	31.0	26.0	34.0
2	30.03775640543993	31.0	30.0	33.0	26.0	34.0
3	29.53795677110727	31.0	30.0	34.0	23.0	34.0
4	33.55617752398127	35.0	33.0	37.0	28.0	37.0
5	33.168645277631676	35.0	33.0	37.0	26.0	37.0
6	33.53527688030656	35.0	33.0	37.0	28.0	37.0
7	33.546497357677765	35.0	33.0	37.0	28.0	37.0
8	33.68674080196358	35.0	33.0	37.0	28.0	37.0
9	35.24349437723846	37.0	34.0	39.0	29.0	39.0
10	35.01497733363388	37.0	34.0	39.0	28.0	39.0
11	35.09258146116663	37.0	34.0	39.0	29.0	39.0
12	34.87255491271571	37.0	33.0	39.0	27.0	39.0
13	35.15068750469607	37.0	34.0	39.0	29.0	39.0
14	35.501089488316175	38.0	33.0	40.0	27.0	41.0
15	35.55214516492599	38.0	34.0	40.0	27.0	41.0
16	35.62219801137075	38.0	34.0	40.0	27.0	41.0
17	35.508866180779926	38.0	33.0	40.0	27.0	41.0
18	35.84720865579683	38.0	34.0	40.0	27.0	41.0
19	35.70310817241466	38.0	34.0	40.0	27.0	41.0
20	35.692426177774436	38.0	34.0	40.0	27.0	41.0
21	35.67073659428457	38.0	34.0	40.0	27.0	41.0
22	35.16873293761114	38.0	33.0	40.0	26.0	41.0
23	35.34808525559146	38.0	34.0	40.0	27.0	41.0
24	35.001966088110805	37.0	33.0	40.0	26.0	41.0
25	35.045983920655196	38.0	33.0	40.0	26.0	41.0
26	34.84874896686453	37.0	33.0	40.0	25.0	41.0
27	34.38480226413204	37.0	33.0	40.0	24.0	41.0
28	34.5284644476169	37.0	33.0	40.0	25.0	41.0
29	34.344228216495104	37.0	32.0	40.0	24.0	41.0
30	34.32972675132116	37.0	33.0	40.0	24.0	41.0
31	34.1969970195607	37.0	32.0	40.0	24.0	41.0
32	33.71618203220878	36.0	31.0	39.0	23.0	41.0
33	33.47853582788589	36.0	31.0	39.0	22.0	40.0
34	33.28599694442357	36.0	31.0	39.0	21.0	40.0
35	33.19764820797956	36.0	31.0	39.0	20.0	40.0
36	33.21243769880031	36.0	31.0	39.0	20.0	41.0
37	33.16970972023944	36.0	31.0	40.0	19.0	41.0
38	32.92557667743632	36.0	30.0	40.0	18.0	41.0
39	32.6420342124377	36.0	30.0	39.0	18.0	41.0
40	31.92254614671776	35.0	30.0	39.0	15.0	40.0
41	32.01931024119017	35.0	30.0	39.0	15.0	40.0
42	31.780274000050092	35.0	28.0	39.0	14.0	40.0
43	30.86045783554988	35.0	26.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	9.0
11	15.0
12	7.0
13	13.0
14	22.0
15	25.0
16	54.0
17	82.0
18	123.0
19	205.0
20	320.0
21	427.0
22	605.0
23	838.0
24	1178.0
25	1508.0
26	1885.0
27	2439.0
28	2875.0
29	3363.0
30	3689.0
31	4196.0
32	4758.0
33	5179.0
34	6022.0
35	6789.0
36	7809.0
37	8689.0
38	10590.0
39	6129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.654995366543943	28.816339820171816	23.962481528790043	22.5661832844942
2	10.896135447191124	22.41340446314524	38.29614045633281	28.394319633330827
3	13.458311418338468	34.09347058381546	36.77837052621034	15.669847471635734
4	8.361509755303429	15.921556841235255	21.106018483732814	54.6109149197285
5	11.84286322538633	40.20211886693215	23.853532697172337	24.10148521050918
6	25.91354221454154	30.27024319382874	15.20399729506349	28.612217296566232
7	17.007288301149597	41.70861822826659	13.232900042577706	28.051193428006112
8	31.935782803616604	20.405990933453552	28.10378941568362	19.554436847246222
9	15.585944348435895	11.542314724371979	12.25110827259749	60.62063265459463
10	15.20399729506349	19.694692814386254	27.582838680592083	37.51847120995818
11	26.062564179627824	17.88764495203747	16.160743356625844	39.88904751170887
12	14.361209206802414	33.486112154682296	16.950935457209408	35.201743181305886
13	40.27475142134395	11.929270919427957	17.026072582462994	30.7699050767651
14	25.382573196082852	30.957747889899068	18.85315701154607	24.806521902472014
15	30.74736393918902	28.120069126155233	15.529591504495704	25.60297543016004
16	30.314073183559998	20.992060510431536	14.880907656473063	33.8129586495354
17	14.69932627044356	37.370701530292784	16.138202219049766	31.791769980213893
18	20.863075112079546	12.168457434818544	23.09339544669021	43.875072006411706
19	21.342700428281614	31.998397074661256	31.105517569564455	15.553384927492676
20	19.778595937586093	29.99098354496957	19.744784231221978	30.485636286222356
21	46.79915846419716	14.006812432689658	23.459062789591005	15.734966313522177
22	25.367545771032134	36.16224609913091	20.389711222981944	18.08049690685501
23	29.333533699000675	17.946502366819445	21.242517594610163	31.477446339569713
24	20.637663736318782	36.48658802314223	30.469356575750744	12.406391664788238
25	19.402910311318156	22.650086407694044	31.776742555163175	26.17026072582463
26	33.9957923209858	21.78851403811957	31.29210809727753	12.9235855436171
27	19.109875522829164	37.81150599844717	29.690435043955215	13.388183434768452
28	26.757582588223507	14.75192225812107	33.646404688556615	24.844090465098805
29	24.656247651964836	22.9856989004934	28.041175144638963	24.316878302902797
30	15.023668194454881	15.330478122573696	42.10058356500614	27.545270117965288
31	28.153880832519345	23.196082851203446	20.148020136749565	28.50201617952764
32	25.7019059784106	18.68785533598818	40.838279860745864	14.77195882485536
33	23.06459288200967	17.206401683071608	39.56345330227665	20.165552132642073
34	15.757507451098254	37.24422070278258	27.35367044856864	19.644601397550527
35	17.853833245673354	13.288000601097002	55.223282490545245	13.6348836626844
36	27.65797580584567	17.1525534099732	21.204949031983368	33.98452175219776
37	19.156210083402208	15.446940666716758	36.00946727778195	29.38738197209908
38	20.67272772810379	12.899792120620132	28.260325093295265	38.167155057980814
39	36.55045457960778	16.809427204648483	25.536604302852705	21.103513912891025
40	14.526510882360308	12.698174167856338	49.37260500413254	23.402709945650813
41	25.01440128234027	21.91374258020888	23.59055275878478	29.48130337866607
42	15.441931525033187	13.136474065168931	50.040073133468574	21.381521276329302
43	25.58920029053022	13.160267488165903	33.12169709720239	28.128835124101485
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	24.0
4	45.0
5	45.0
6	72.5
7	100.0
8	117.0
9	134.0
10	192.0
11	250.0
12	250.0
13	403.0
14	556.0
15	1374.5
16	2193.0
17	2166.5
18	2140.0
19	2140.0
20	1796.0
21	1452.0
22	1117.0
23	782.0
24	685.5
25	589.0
26	589.0
27	515.0
28	441.0
29	440.5
30	440.0
31	428.0
32	416.0
33	416.0
34	487.5
35	559.0
36	540.5
37	522.0
38	602.0
39	682.0
40	682.0
41	896.5
42	1111.0
43	1451.5
44	1792.0
45	2307.0
46	2822.0
47	2822.0
48	4393.0
49	5964.0
50	10645.5
51	15327.0
52	10252.5
53	5178.0
54	5178.0
55	12026.5
56	18875.0
57	15309.5
58	11744.0
59	7128.0
60	2512.0
61	2512.0
62	2002.5
63	1493.0
64	1159.5
65	826.0
66	649.5
67	473.0
68	473.0
69	356.0
70	239.0
71	174.5
72	110.0
73	76.0
74	42.0
75	42.0
76	33.5
77	25.0
78	16.0
79	7.0
80	5.0
81	3.0
82	3.0
83	3.0
84	3.0
85	2.5
86	2.0
87	1.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	79854.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.540260976281715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.61640596580575	22.752773812207277
2	7.3754092397235365	4.062413905377314
3	2.9101491451436883	2.4043880081148097
4	1.682429974536195	1.8533824229218323
5	1.0913059294288832	1.502742505071756
6	0.7138959621680611	1.1796528664813284
7	0.5456529647144416	1.0519197535502292
8	0.40014550745725724	0.8816089363087636
9	0.2955620225536559	0.732586971222481
>10	2.05529283375773	10.788438900994315
>50	0.15460167333575847	2.803867057379718
>100	0.10003637686431431	4.300348135347008
>500	0.018188432157148052	3.3097903674205424
>1k	0.03182975627500909	20.61387031332181
>5k	0.009094216078574026	21.762216044280812
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	8909	11.156610814736895	TruSeq Adapter, Index 1 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	8469	10.605605229543917	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	4597	5.756756079845719	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	3392	4.247752147669496	RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	3134	3.9246625090790697	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	1611	2.0174318130588325	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1426	1.7857590101936032	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1217	1.5240313572269393	No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	1084	1.3574773962481528	No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT	755	0.9454754927743132	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	689	0.8628246549953665	No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	642	0.8039672402133895	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	557	0.6975229794374734	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	345	0.43203847020812985	No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT	344	0.43078618478723674	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	242	0.30305307185613745	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	222	0.27800736343827487	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	185	0.23167280286522904	No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	176	0.2204022340771909	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	155	0.19410424023843514	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	151	0.18909509855486262	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	133	0.1665539609787863	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	131	0.164049390137	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	131	0.164049390137	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	125	0.15653567761164125	No Hit
TCGCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	118	0.14776967966538934	TruSeq Adapter, Index 1 (95% over 21bp)
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	117	0.1465173942444962	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	115	0.14401282340270996	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	112	0.14025596714003055	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	110	0.13775139629824432	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	109	0.13649911087735117	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	105	0.13148996919377864	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104	0.1302376837728855	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	103	0.12898539835199238	No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT	101	0.12648082751020612	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	95	0.11896711498484734	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	93	0.11646254414306109	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	92	0.11521025872216796	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	88	0.11020111703859545	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	82	0.10268740451323664	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	80	0.1001828336714504	No Hit
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	80	0.1001828336714504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.00125228542089313	0.0
25	0.0	0.0	0.0	0.00125228542089313	0.0
26	0.0	0.0	0.0	0.00125228542089313	0.0
27	0.0	0.0	0.0	0.00125228542089313	0.0
28	0.0	0.0	0.0	0.0037568562626793898	0.0
29	0.0	0.0	0.0	0.01127056878803817	0.0
30	0.0	0.0	0.0	0.01627971047161069	0.0
31	0.0	0.0	0.0	0.02755027925964886	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACAC	20	0.0018316995	37.0	3
TGATACA	20	0.0018316995	37.0	2
TTTACAC	40	3.7573045E-8	37.0	2
CTTATAC	245	0.0	35.4898	1
CGTCTTC	415	0.0	32.987953	37
CTTTACA	50	2.667548E-7	29.599998	1
TATAAGA	25	0.0054661464	29.599998	13
AACTTTA	25	0.0054661464	29.599998	7
GTATAAG	25	0.0054661464	29.599998	12
TAACTTT	25	0.0054661464	29.599998	6
TTAACTT	25	0.0054661464	29.599998	5
TACTTTA	25	0.0054661464	29.599998	36
TTATACA	295	0.0	29.474579	2
GGTATCA	110	0.0	28.590908	1
TTCGCTC	40	5.8722406E-5	27.75	36
CGGGAGC	40	5.8722406E-5	27.75	16
TTACACA	55	6.1399805E-7	26.90909	3
TCGCTCG	35	8.7970204E-4	26.42857	37
GTATCAA	410	0.0	26.170732	1
CCGTCTT	1320	0.0	25.227272	37
>>END_MODULE