FastQCFastQC Report
Fri 10 Feb 2017
ERR1631085.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631085.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173629
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG145318.368993658893388No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT98895.695477138035697Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT88655.105713907238998No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC78544.523437904958273No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC38272.2041248869716465No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC33931.9541666426691393Illumina PCR Primer Index 11 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26091.502629169090417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21761.2532468654429847No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG18531.0672180338537915No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA11960.6888250234695816No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT11050.6364144238577657No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT9550.5500233255965306No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9490.5465676816660812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9190.5292894620138341No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7280.41928479689452797No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6360.3662982566276371No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5990.34498845238986575No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5470.31503953832597087No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4320.24880636299235726No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4310.24823042233728235No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4210.24247101578653338No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4090.23555972792563457No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3650.21021833910233892No Hit
ATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCT3490.20100328862114047Illumina PCR Primer Index 11 (95% over 21bp)
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3180.18314912831381855No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3180.18314912831381855No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2990.1722062558673954No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2910.16759873062679623No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2820.1624152647311221No Hit
CTTTACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA2810.1618393240760472No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2800.1612633834209723No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2770.1595355614557476No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2770.1595355614557476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.1578077394905229No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2670.1537761549049986No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2660.15320021424992367No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2560.14744080769917467No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2470.14225734180350055No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2470.14225734180350055No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2370.13649793525275156No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2300.13246635066722726No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2290.13189041001215235No Hit
CTGATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT2270.13073852870200256No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2220.12785882542662802No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2090.12037159691065433No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2080.11979565625557943No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2080.11979565625557943No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2080.11979565625557943No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2000.11518813101498021No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2000.11518813101498021No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG1980.11403624970483042No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.1134603090497555No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG1960.1128843683946806No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1950.11230842773960571No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG1940.1117324870845308No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC1890.1088527838091563No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1880.1082768431540814No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1770.1019414959482575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATGT200.001837635937.01
TTTACAC403.801324E-837.02
TTACGGG200.001837635937.03
AGCTTCT251.2293132E-436.99999637
ATTACGG251.2293132E-436.9999962
AGCAACG251.2293132E-436.9999962
AAGCTTC251.2293132E-436.99999636
CTTATAC10600.036.1273571
CAGACAC603.45608E-1133.91666418
TTATACA11500.033.32
ACAGACA658.54925E-1131.30769217
GACAGAC658.54925E-1131.30769216
AGACAGA658.54925E-1131.30769215
GCAACGC303.587014E-430.8333323
TAGCAAC303.587014E-430.8333321
CGTCTTC4600.030.56521837
TATACAC12800.030.3515623
GATTACG250.005483658529.5999981
GAGACAG754.5656634E-1027.13333114
TTACACA556.210412E-726.909093
Produced by FastQC (version 0.11.5)