FastQCFastQC Report
Fri 10 Feb 2017
ERR1631084.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631084.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151619
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT80835.331126046207929RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG67834.473713716618629No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC40732.686338783397859No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC26161.7253774263120056RNA PCR Primer, Index 46 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC25551.6851450016158926No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT12910.8514763980767581No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG10310.6799939321588983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.5724876169873169No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7690.5071923703493625No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT6790.44783305522394956No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT5440.35879408253582995No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA4790.31592346605636495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3250.2143530823973249No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT3010.19852393169721474No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA2010.13256913711342247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC2950.035.7457637
GGTATCA900.030.8333341
CAGTACT303.5848864E-430.8333324
GTGCAAA250.00548149129.611
GGATAAG250.00548149129.61
CAGTGCA250.00548149129.69
ATGCCGC250.00548149129.634
CTTATAC1900.028.2368431
CCGTCTT11750.027.39574437
GCCGTCT12150.026.49382836
TGATATA358.8337803E-426.428572
GTGTAGG358.8337803E-426.428571
TTATACA2150.025.8139532
ATACACA10150.025.3349761
TACACAT10150.025.152712
CTACACA451.315824E-424.6666684
TGCCGTC14800.021.7535
GTATCAA2300.021.7173921
TATATAT603.6988946E-521.5833322
GTATATA705.0538856E-621.1428571
Produced by FastQC (version 0.11.5)