##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631084.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151619
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.6443387702069	31.0	30.0	31.0	25.0	34.0
2	29.615958422097496	31.0	30.0	31.0	25.0	34.0
3	29.111806567778444	31.0	28.0	31.0	23.0	34.0
4	33.202388882659825	35.0	33.0	37.0	28.0	37.0
5	32.345108462659695	35.0	32.0	37.0	25.0	37.0
6	32.71750901931816	35.0	32.0	37.0	26.0	37.0
7	32.87085391672548	35.0	32.0	37.0	26.0	37.0
8	32.977080708882134	35.0	32.0	37.0	26.0	37.0
9	34.44749009029211	37.0	33.0	39.0	27.0	39.0
10	34.026157671531934	37.0	32.0	39.0	27.0	39.0
11	34.33625732922655	37.0	32.0	39.0	27.0	39.0
12	34.13888760643455	37.0	32.0	39.0	27.0	39.0
13	34.413998245602464	37.0	33.0	39.0	27.0	39.0
14	34.96309169695091	37.0	33.0	39.0	27.0	40.0
15	34.97852511888352	37.0	33.0	39.0	27.0	40.0
16	35.05051477717173	37.0	33.0	39.0	27.0	40.0
17	34.87029989645097	37.0	33.0	39.0	27.0	40.0
18	35.118270137647656	37.0	33.0	40.0	27.0	41.0
19	35.06187878827851	37.0	33.0	40.0	27.0	40.0
20	35.02860459441099	37.0	33.0	40.0	27.0	40.0
21	34.96896826914833	37.0	33.0	39.0	27.0	40.0
22	34.67899801476068	37.0	32.0	39.0	26.0	40.0
23	34.79362744774731	37.0	33.0	39.0	27.0	40.0
24	34.63406301321074	37.0	32.0	39.0	26.0	40.0
25	34.54934407956787	37.0	32.0	39.0	25.0	40.0
26	34.22059240596495	37.0	32.0	39.0	25.0	40.0
27	34.04918249032114	37.0	32.0	39.0	24.0	40.0
28	34.02254334878874	37.0	32.0	39.0	24.0	40.0
29	33.94849590090951	37.0	32.0	39.0	24.0	40.0
30	33.8436409684802	36.0	31.0	39.0	24.0	40.0
31	33.76840633429847	36.0	31.0	39.0	24.0	40.0
32	33.481832751831895	36.0	31.0	39.0	24.0	40.0
33	33.17181223989078	36.0	30.0	39.0	22.0	40.0
34	33.024106477420375	36.0	30.0	39.0	22.0	40.0
35	33.00637123315679	36.0	30.0	39.0	22.0	40.0
36	33.020920860841976	36.0	30.0	39.0	22.0	40.0
37	32.96798554270903	36.0	30.0	39.0	22.0	40.0
38	32.865643487953356	36.0	30.0	39.0	21.0	40.0
39	32.614256788397235	35.0	30.0	38.0	20.0	40.0
40	32.16142435974383	35.0	30.0	38.0	19.0	40.0
41	32.255911198464574	35.0	30.0	38.0	19.0	40.0
42	32.15381977192832	35.0	30.0	38.0	19.0	40.0
43	31.316306003864952	34.0	28.0	38.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	15.0
11	12.0
12	9.0
13	12.0
14	12.0
15	29.0
16	28.0
17	58.0
18	108.0
19	197.0
20	320.0
21	502.0
22	828.0
23	1229.0
24	1879.0
25	2558.0
26	3672.0
27	4740.0
28	6138.0
29	7435.0
30	8999.0
31	10608.0
32	11997.0
33	13257.0
34	14118.0
35	14717.0
36	14645.0
37	14430.0
38	13577.0
39	5485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.303464605359487	28.08289198583291	19.769949676491734	20.84369373231587
2	14.588541013989012	24.328745078123454	37.02702167934098	24.055692228546555
3	16.082417111311905	31.660280044057803	34.923063732118	17.334239112512286
4	12.157447285630429	18.508234456103786	32.249915907636904	37.08440235062888
5	11.433923189046228	40.507456189527694	31.207170605267148	16.851450016158925
6	26.75192423113198	37.28688357000112	17.531443948317822	18.429748250549075
7	20.910308074845503	38.23597306406189	20.268567923545202	20.585150937547407
8	27.226139204189447	30.761975741826554	24.4791220097745	17.5327630442095
9	20.699912280123204	14.626135246901772	17.86517520891181	46.80877726406321
10	14.145984342331767	25.85427947684657	32.43392978452568	27.565806396295976
11	28.82290478106306	22.522243254473384	21.49136981512871	27.163482149334843
12	17.221456413774	30.913012221423436	25.811408860367102	26.054122504435462
13	32.0810716335024	18.686971949425864	22.82101847393796	26.410937943133778
14	23.135622844102652	26.481509573338435	23.719322776169214	26.663544806389698
15	25.083927476107874	31.104940673662274	20.31011944413299	23.501012406096862
16	26.02510239481859	27.100165546534406	20.817971362428192	26.056760696218813
17	18.022147620021236	34.884809951259406	22.32965525428871	24.763387174430644
18	21.257889842302085	22.94369439186382	25.155818202204212	30.642597563629888
19	22.074410199249435	30.87343934467316	29.30767252125393	17.744477934823472
20	19.42038926519763	32.883741483587144	23.613135556889308	24.08273369432591
21	27.37123975227379	29.253589589695224	25.055566914436845	18.31960374359414
22	25.0252277089283	33.25440742914807	23.936314050349893	17.78405081157375
23	28.20358925992125	24.932231448565155	24.474505174153634	22.389674117359963
24	22.213574815821236	28.770800493341863	26.41357613491713	22.602048555919772
25	26.22362632651581	29.232484055428408	23.245107803111747	21.298781814944036
26	19.6598051695368	33.48195147046214	28.16863321879184	18.689610141209215
27	22.88169688495505	34.231197936934024	25.347087106497206	17.540018071613716
28	20.73618741714429	29.43166753507146	30.03647300140484	19.795672046379412
29	22.95754489872641	25.298280558505198	26.598909107697583	25.1452654350708
30	25.500761777877447	24.992250311636404	31.09240926269135	18.4145786477948
31	20.55876901971389	26.594292272076718	24.135497529992943	28.71144117821645
32	29.525982891326287	24.904530434839963	27.817753711606063	17.75173296222769
33	19.23835403214637	28.669230109682825	32.73138590809859	19.36102995007222
34	19.63408279964912	32.48471497635521	26.27639016218284	21.604812061812833
35	20.585150937547407	23.185088940040497	38.78999333856575	17.439766783846352
36	23.98643969423357	25.62937362731584	24.311596831531666	26.07258984691892
37	20.660998951318767	23.661282556935475	31.25201986558413	24.42569862616163
38	21.280314472460578	22.548625172306902	28.175888246196056	27.995172109036464
39	28.55446876710703	24.744919831947186	26.515146518576167	20.185464882369626
40	18.461406551949295	22.379780898172395	37.7439502964668	21.414862253411513
41	22.706257131362165	26.390491956812802	25.69466887395379	25.208582037871242
42	18.768096346763926	22.57104980246539	37.70239877587901	20.958455074891667
43	23.405377953950364	21.97415891148207	31.068005988695347	23.552457145872218
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	6.5
2	7.0
3	27.0
4	47.0
5	47.0
6	65.0
7	83.0
8	97.0
9	111.0
10	149.5
11	188.0
12	188.0
13	283.0
14	378.0
15	931.5
16	1485.0
17	1607.5
18	1730.0
19	1730.0
20	1764.5
21	1799.0
22	2012.0
23	2225.0
24	2769.5
25	3314.0
26	3314.0
27	3755.0
28	4196.0
29	4720.5
30	5245.0
31	5834.0
32	6423.0
33	6423.0
34	6833.5
35	7244.0
36	7652.0
37	8060.0
38	8223.5
39	8387.0
40	8387.0
41	8529.5
42	8672.0
43	8539.0
44	8406.0
45	8375.0
46	8344.0
47	8344.0
48	8837.5
49	9331.0
50	12867.0
51	16403.0
52	12063.5
53	7724.0
54	7724.0
55	13671.0
56	19618.0
57	15711.5
58	11805.0
59	7734.0
60	3663.0
61	3663.0
62	3103.0
63	2543.0
64	2097.5
65	1652.0
66	1357.5
67	1063.0
68	1063.0
69	825.5
70	588.0
71	488.0
72	388.0
73	309.0
74	230.0
75	230.0
76	180.5
77	131.0
78	105.0
79	79.0
80	51.0
81	23.0
82	23.0
83	19.5
84	16.0
85	11.0
86	6.0
87	6.0
88	6.0
89	6.0
90	3.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	151619.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.18667185511051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.53053662202645	57.835099822581604
2	4.882936860014822	6.170730581259605
3	1.2817970209701157	2.429774632466907
4	0.6325480412930702	1.5987442207111247
5	0.39247205202342306	1.2399501381752946
6	0.23485694602465473	0.8903897268811957
7	0.19727983466070997	0.8725819323435716
8	0.13882655031679592	0.7017590143715497
9	0.10229324760184964	0.5817212882290478
>10	0.5647004791081699	6.2155798415765835
>50	0.019832364330970846	0.7696924527928558
>100	0.010438086489984656	1.374497919126231
>500	0.004175234595993863	1.8863071250964587
>1k	0.005219043244992328	7.628331541561415
>5k	0.0020876172979969313	9.804839762826559
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	8083	5.331126046207929	RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	6783	4.473713716618629	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	4073	2.686338783397859	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC	2616	1.7253774263120056	RNA PCR Primer, Index 46 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	2555	1.6851450016158926	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	1291	0.8514763980767581	No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG	1031	0.6799939321588983	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	868	0.5724876169873169	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	769	0.5071923703493625	No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	679	0.44783305522394956	No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT	544	0.35879408253582995	No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	479	0.31592346605636495	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	325	0.2143530823973249	No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT	301	0.19852393169721474	No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	201	0.13256913711342247	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	6.595479458379227E-4	0.0
21	0.0	0.0	0.0	6.595479458379227E-4	0.0
22	0.0	0.0	0.0	6.595479458379227E-4	0.0
23	0.0	0.0	0.0	6.595479458379227E-4	0.0
24	0.0	0.0	0.0	6.595479458379227E-4	0.0
25	0.0	0.0	0.0	0.001978643837513768	0.0
26	0.0	0.0	0.0	0.001978643837513768	0.0
27	0.0	0.0	0.0	0.001978643837513768	0.0
28	0.0	0.0	0.0	0.005276383566703381	0.0
29	0.0	0.0	0.0	0.0065954794583792265	0.0
30	0.0	0.0	0.0	0.011212315079244686	0.0
31	0.0	0.0	0.0	0.025062821941841062	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	295	0.0	35.74576	37
GGTATCA	90	0.0	30.833334	1
CAGTACT	30	3.5848864E-4	30.833332	4
GTGCAAA	25	0.005481491	29.6	11
GGATAAG	25	0.005481491	29.6	1
CAGTGCA	25	0.005481491	29.6	9
ATGCCGC	25	0.005481491	29.6	34
CTTATAC	190	0.0	28.236843	1
CCGTCTT	1175	0.0	27.395744	37
GCCGTCT	1215	0.0	26.493828	36
TGATATA	35	8.8337803E-4	26.42857	2
GTGTAGG	35	8.8337803E-4	26.42857	1
TTATACA	215	0.0	25.813953	2
ATACACA	1015	0.0	25.334976	1
TACACAT	1015	0.0	25.15271	2
CTACACA	45	1.315824E-4	24.666668	4
TGCCGTC	1480	0.0	21.75	35
GTATCAA	230	0.0	21.717392	1
TATATAT	60	3.6988946E-5	21.583332	2
GTATATA	70	5.0538856E-6	21.142857	1
>>END_MODULE