FastQCFastQC Report
Fri 10 Feb 2017
ERR1631083.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631083.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835548
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT244372.924667403907376No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG182182.180365460751507No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT149611.7905614040126958RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC95521.1432018268250297No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC56590.6772800605111855RNA PCR Primer, Index 27 (95% over 24bp)
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA47400.5672923638139281No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC43910.525523369094295RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31250.3740060415439927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31010.3711336751449348No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG21920.2623427977806182No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA17250.206451334932284No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC15240.18239526634017433No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14600.17473562260935338No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT14520.17377816714300076No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA13770.16480202214594494No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG12360.14792686955148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11600.13883104262113008No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT11350.13583899428877816No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG10970.1312910808236032No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA10720.12829903249125127No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT9660.11561274756207902No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8510.10184932523326008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC28600.034.4125861
TTATACA30550.032.5793762
GATTACG750.032.0666661
TATACAC32250.031.034113
CGTCTTC7850.029.92993737
CGCCGTT405.938219E-527.7525
GTATCAA13750.025.5636351
CCGTCTT23750.024.84842137
TTACCGG601.3366753E-624.66666831
GGTATCA4550.024.3956051
GCCGTCT24350.024.2361436
GCTTTAT7750.023.154841
CGAAAAT502.7012458E-422.237
TTACGGG1005.329639E-1022.23
AGTCCGC603.7247177E-521.58333421
TGCCGTC28100.021.06761635
TTTATTG9000.020.9666673
TCGCCGA450.003824947220.5555551
CGCATAC450.003824947220.55555512
GCGCATA450.003824947220.55555511
Produced by FastQC (version 0.11.5)