##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631083.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	835548
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.494463513765815	31.0	31.0	34.0	28.0	34.0
2	31.505125977202987	31.0	31.0	34.0	28.0	34.0
3	30.988377687457813	31.0	31.0	34.0	27.0	34.0
4	35.11187029350798	37.0	35.0	37.0	32.0	37.0
5	35.30521645674455	37.0	35.0	37.0	33.0	37.0
6	35.48914484865023	37.0	35.0	37.0	33.0	37.0
7	35.49365685753541	37.0	35.0	37.0	33.0	37.0
8	35.51709776099039	37.0	35.0	37.0	33.0	37.0
9	37.30425660763954	39.0	37.0	39.0	34.0	39.0
10	37.142346101001976	39.0	37.0	39.0	33.0	39.0
11	37.19503008803803	39.0	37.0	39.0	33.0	39.0
12	37.119915313064	39.0	37.0	39.0	33.0	39.0
13	37.21355565449262	39.0	37.0	39.0	34.0	39.0
14	38.3115763546798	40.0	38.0	41.0	34.0	41.0
15	38.31975422118179	40.0	38.0	41.0	34.0	41.0
16	38.321507561504546	40.0	38.0	41.0	34.0	41.0
17	38.2862887589941	40.0	38.0	41.0	33.0	41.0
18	38.33931623317871	40.0	38.0	41.0	34.0	41.0
19	38.337942284584486	40.0	38.0	41.0	34.0	41.0
20	38.31654315491151	40.0	38.0	41.0	34.0	41.0
21	38.24369874621207	40.0	38.0	41.0	34.0	41.0
22	38.199740768932486	40.0	38.0	41.0	34.0	41.0
23	38.22797134335789	40.0	38.0	41.0	34.0	41.0
24	38.19103390828534	40.0	38.0	41.0	33.0	41.0
25	38.14148199744359	40.0	37.0	41.0	33.0	41.0
26	38.02674173117523	40.0	37.0	41.0	33.0	41.0
27	37.94745005672924	40.0	37.0	41.0	33.0	41.0
28	37.87642840387387	40.0	37.0	41.0	33.0	41.0
29	37.88290199964574	40.0	37.0	41.0	33.0	41.0
30	37.789292775519776	40.0	37.0	41.0	33.0	41.0
31	37.684276666331556	40.0	37.0	41.0	32.0	41.0
32	37.67484453316865	40.0	37.0	41.0	32.0	41.0
33	37.65726684762575	40.0	37.0	41.0	33.0	41.0
34	37.59645765413836	40.0	37.0	41.0	32.0	41.0
35	37.590708134062915	40.0	37.0	41.0	32.0	41.0
36	37.55615715674025	40.0	37.0	41.0	32.0	41.0
37	37.46208236989377	40.0	37.0	41.0	32.0	41.0
38	37.416872519591934	40.0	37.0	41.0	32.0	41.0
39	37.301785175717015	39.0	36.0	41.0	31.0	41.0
40	37.16858516805737	39.0	36.0	41.0	31.0	41.0
41	37.151956560245495	39.0	36.0	41.0	31.0	41.0
42	37.07989846184779	39.0	36.0	41.0	31.0	41.0
43	36.36120725559753	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	21.0
11	19.0
12	11.0
13	17.0
14	17.0
15	11.0
16	18.0
17	19.0
18	23.0
19	48.0
20	73.0
21	144.0
22	332.0
23	640.0
24	1157.0
25	1916.0
26	3213.0
27	5071.0
28	7347.0
29	10259.0
30	14002.0
31	18713.0
32	23945.0
33	31263.0
34	41434.0
35	55073.0
36	76064.0
37	113097.0
38	196060.0
39	235526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.5454192936851	22.21703600511281	15.276920057255836	24.96062464394625
2	13.249268743387574	21.33857061473428	40.994891975087015	24.417268666791138
3	14.361592631422731	28.310282592980897	35.31969438021514	22.008430395381236
4	11.107440865156759	19.345746743454594	36.95514799867871	32.59166439270994
5	12.668811366911298	37.549249115550516	35.72601454374853	14.055924973789658
6	27.71761765930862	40.04342060539909	16.9145279505187	15.324433784773586
7	24.030456658384676	32.94855591779287	21.875344085558222	21.14564333826423
8	25.48363469244137	33.34147170479733	23.046910530573946	18.127983072187355
9	23.3327109872802	14.39821530301072	18.753680219448793	43.51539349026029
10	14.569480149554545	28.982176966493846	32.56772800605112	23.88061487790049
11	31.164577020111352	22.473993115895198	25.291186143704493	21.07024372028896
12	20.715147424205433	26.136379956627266	27.281257330518415	25.867215288648886
13	29.99109566416292	19.374470407445173	26.534322384830077	24.10011154356183
14	21.63992972276877	22.44383326870509	25.919157247698514	29.99707976082762
15	24.766859593943135	28.466228152063078	21.635142445437005	25.131769808556783
16	25.793132171939853	26.182816546745368	22.910951854351875	25.113099426962904
17	21.33857061473428	31.201558737499223	23.43659490537946	24.023275742387032
18	25.36108039274823	24.06935328670525	25.32086726316142	25.248699057385092
19	26.8693121161202	26.28155414171298	24.70211166803104	22.147022074135776
20	24.943869173285076	25.317635850962482	23.95960495387458	25.77889002187786
21	25.526720188427237	25.879063800045	24.485966096501937	24.10824991502583
22	21.215178541508088	31.054948369213975	25.742267350289872	21.987605738988066
23	23.8885138854979	24.170484520338746	26.53527984029643	25.40572175386692
24	24.67530291497317	26.444201889059638	24.83053038245559	24.04996481351161
25	22.28896484702255	28.168698865893997	25.525523369094294	24.016812917989153
26	26.659868732855564	26.117948938899982	26.296155337574863	20.926026990669598
27	23.466156342903098	28.329072656508064	25.773504334879625	22.431266665709213
28	21.05145365676179	26.330025324697086	28.34570844523594	24.272812573305185
29	24.43833268705089	25.12997457955737	24.933217481221906	25.498475252169833
30	24.626831731989064	24.213809380191204	28.78745446102438	22.37190442679535
31	21.449276403031302	27.161575397224336	26.08300181437811	25.30614638536625
32	21.63609990090336	25.685777477775062	26.41942772886776	26.258694892453814
33	19.69426053320695	25.020345928659992	33.70614255554438	21.579250982588672
34	21.597203272582785	27.369702279222736	25.792892808073265	25.240201640121214
35	20.9784476774524	24.04960576771173	30.677950279337633	24.293996275498237
36	22.492783179422368	24.848362990516403	28.422424564477446	24.236429265583784
37	22.132899606007076	26.512300909103963	28.48657408072307	22.86822540416589
38	22.25042726450186	23.720600133086307	28.930474371310805	25.098498231101026
39	25.23888513885498	24.72497091729021	25.936511128026158	24.099632815828652
40	20.87827389928526	23.4641217500371	33.29611225207888	22.361492098598763
41	22.15097157793448	25.34863347168565	25.64723989525437	26.853155055125498
42	23.400690325391242	23.781996964866174	30.25643051027589	22.560882199466697
43	23.35724578360549	24.04733181097914	29.964645956904928	22.63077644851044
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	24.5
2	31.0
3	122.5
4	214.0
5	214.0
6	295.5
7	377.0
8	358.5
9	340.0
10	486.0
11	632.0
12	632.0
13	972.5
14	1313.0
15	3187.5
16	5062.0
17	5660.0
18	6258.0
19	6258.0
20	6518.0
21	6778.0
22	8011.5
23	9245.0
24	11810.5
25	14376.0
26	14376.0
27	17271.0
28	20166.0
29	23108.5
30	26051.0
31	29214.0
32	32377.0
33	32377.0
34	35370.5
35	38364.0
36	40887.0
37	43410.0
38	44873.5
39	46337.0
40	46337.0
41	47440.0
42	48543.0
43	49084.0
44	49625.0
45	51461.5
46	53298.0
47	53298.0
48	55007.0
49	56716.0
50	56417.5
51	56119.0
52	74885.0
53	93651.0
54	93651.0
55	70686.5
56	47722.0
57	54813.0
58	61904.0
59	52800.5
60	43697.0
61	43697.0
62	34656.0
63	25615.0
64	22233.5
65	18852.0
66	15572.5
67	12293.0
68	12293.0
69	10263.0
70	8233.0
71	6896.5
72	5560.0
73	3457.5
74	1355.0
75	1355.0
76	918.5
77	482.0
78	380.5
79	279.0
80	201.0
81	123.0
82	123.0
83	90.5
84	58.0
85	54.5
86	51.0
87	34.5
88	18.0
89	18.0
90	11.5
91	5.0
92	2.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	835548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.99221793904963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07187759851276	36.36691027360656
2	12.655874194186016	11.641434482963946
3	3.4737368175602787	4.792945823283991
4	1.4324093485072888	2.6351873173791733
5	0.7138436440864563	1.6415626226614841
6	0.45581414396110126	1.2578342069256188
7	0.3116129876493347	1.0032240708424611
8	0.2354276205835052	0.8662270747798767
9	0.1719490791279503	0.7117487569504424
>10	1.2207942523755515	11.170325418395338
>50	0.1430523923112679	4.614254653721786
>100	0.10188233294770085	8.914394398604438
>500	0.006514215661567148	1.9667154194377077
>1k	0.003908529396940288	3.6895538125786795
>5k	5.211372529253718E-4	1.8229058707614998
>10k+	7.817058793880577E-4	6.904775797106999
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	24437	2.924667403907376	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG	18218	2.180365460751507	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	14961	1.7905614040126958	RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	9552	1.1432018268250297	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	5659	0.6772800605111855	RNA PCR Primer, Index 27 (95% over 24bp)
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	4740	0.5672923638139281	No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	4391	0.525523369094295	RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3125	0.3740060415439927	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3101	0.3711336751449348	No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG	2192	0.2623427977806182	No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA	1725	0.206451334932284	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1524	0.18239526634017433	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1460	0.17473562260935338	No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT	1452	0.17377816714300076	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1377	0.16480202214594494	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1236	0.14792686955148	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1160	0.13883104262113008	No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT	1135	0.13583899428877816	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	1097	0.1312910808236032	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	1072	0.12829903249125127	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	966	0.11561274756207902	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	851	0.10184932523326008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.1968193329407766E-4	0.0
9	0.0	0.0	0.0	1.1968193329407766E-4	0.0
10	0.0	0.0	0.0	3.59045799882233E-4	0.0
11	0.0	0.0	0.0	3.59045799882233E-4	0.0
12	0.0	0.0	0.0	3.59045799882233E-4	0.0
13	0.0	0.0	0.0	4.7872773317631065E-4	0.0
14	0.0	0.0	0.0	4.7872773317631065E-4	0.0
15	0.0	0.0	0.0	4.7872773317631065E-4	0.0
16	0.0	0.0	0.0	4.7872773317631065E-4	0.0
17	0.0	0.0	0.0	4.7872773317631065E-4	0.0
18	0.0	0.0	0.0	4.7872773317631065E-4	0.0
19	0.0	0.0	0.0	4.7872773317631065E-4	0.0
20	0.0	0.0	0.0	4.7872773317631065E-4	0.0
21	0.0	0.0	0.0	9.574554663526213E-4	0.0
22	0.0	0.0	0.0	0.0015558651328230096	0.0
23	0.0	0.0	0.0	0.00203459286599932	0.0
24	0.0	0.0	0.0	0.002513320599175631	0.0
25	0.0	0.0	0.0	0.0026330025324697087	0.0
26	0.0	0.0	0.0	0.0026330025324697087	0.0
27	0.0	0.0	0.0	0.003949503798704563	0.0
28	0.0	0.0	0.0	0.009813918530114369	0.0
29	0.0	0.0	0.0	0.023816704725521453	0.0
30	0.0	0.0	0.0	0.04140994891975087	0.0
31	0.0	0.0	0.0	0.08700876550479446	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2860	0.0	34.412586	1
TTATACA	3055	0.0	32.579376	2
GATTACG	75	0.0	32.066666	1
TATACAC	3225	0.0	31.03411	3
CGTCTTC	785	0.0	29.929937	37
CGCCGTT	40	5.938219E-5	27.75	25
GTATCAA	1375	0.0	25.563635	1
CCGTCTT	2375	0.0	24.848421	37
TTACCGG	60	1.3366753E-6	24.666668	31
GGTATCA	455	0.0	24.395605	1
GCCGTCT	2435	0.0	24.23614	36
GCTTTAT	775	0.0	23.15484	1
CGAAAAT	50	2.7012458E-4	22.2	37
TTACGGG	100	5.329639E-10	22.2	3
AGTCCGC	60	3.7247177E-5	21.583334	21
TGCCGTC	2810	0.0	21.067616	35
TTTATTG	900	0.0	20.966667	3
TCGCCGA	45	0.0038249472	20.555555	1
CGCATAC	45	0.0038249472	20.555555	12
GCGCATA	45	0.0038249472	20.555555	11
>>END_MODULE