FastQCFastQC Report
Fri 10 Feb 2017
ERR1631082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993001
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT175201.7643486763860259No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG134831.3578032650520997No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT99541.0024159089467182Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC72250.7275924193429815No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55880.5627386075139904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53420.5379652185647346No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC37870.3813692030521621Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC33900.34138938430072074No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA29290.2949644562291478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19450.1958709004321244No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG17700.1782475546348896No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT16450.16565945049400757No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16010.16122843783641708No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT15590.15699883484508073No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA13170.1326282652283331No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12980.13071487339891905No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12770.12860007190325085No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12190.12275919158188159No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11950.12034227558683223No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10840.10916403910972901No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT10770.1084591052778396No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC9980.10050342346080215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGC200.001841815437.030
ATTACGG650.037.02
CTTATAC21450.032.2564131
TCGCCGA303.5991296E-430.8333321
CATCGTA303.5991296E-430.8333329
CGTCTTC5200.030.24038537
TAGACGA250.005495987429.631
TTATACA23700.029.1160342
GATTACG851.8189894E-1228.2941191
TATACAC24850.028.215293
GGTATCA7300.027.3698641
TTACGGG905.456968E-1226.7222213
CGCCGTT706.5901986E-926.42857225
GTATCAA25300.026.3972341
AGGACCG809.786163E-1025.43755
TACGGCC751.373337E-824.66666619
CCGTCTT15150.024.17821537
GCCGTCT15300.024.06209236
GCCGTTT903.8271537E-922.6111126
CGTTACT502.7017432E-422.227
Produced by FastQC (version 0.11.5)