##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631082.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	993001
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.489348953324317	31.0	31.0	34.0	28.0	34.0
2	31.472818254966512	31.0	31.0	34.0	28.0	34.0
3	30.939701974116844	31.0	31.0	34.0	27.0	34.0
4	35.09359708600495	37.0	35.0	37.0	32.0	37.0
5	35.3081054299039	37.0	35.0	37.0	33.0	37.0
6	35.50325830487583	37.0	35.0	37.0	33.0	37.0
7	35.51022808637655	37.0	35.0	37.0	33.0	37.0
8	35.52038920403907	37.0	35.0	37.0	33.0	37.0
9	37.29659990271913	39.0	37.0	39.0	34.0	39.0
10	37.13482765878383	39.0	37.0	39.0	33.0	39.0
11	37.1989393766975	39.0	37.0	39.0	33.0	39.0
12	37.13144296934243	39.0	37.0	39.0	33.0	39.0
13	37.20039254743953	39.0	37.0	39.0	33.0	39.0
14	38.30433403390329	40.0	38.0	41.0	34.0	41.0
15	38.314094346329966	40.0	38.0	41.0	34.0	41.0
16	38.31047702872404	40.0	38.0	41.0	34.0	41.0
17	38.31467138502378	40.0	38.0	41.0	33.0	41.0
18	38.358647171553706	40.0	38.0	41.0	34.0	41.0
19	38.38195832632596	40.0	38.0	41.0	34.0	41.0
20	38.383060037200366	40.0	38.0	41.0	34.0	41.0
21	38.3124176108584	40.0	38.0	41.0	34.0	41.0
22	38.23498667171533	40.0	38.0	41.0	34.0	41.0
23	38.24473792070703	40.0	38.0	41.0	34.0	41.0
24	38.20312366251394	40.0	38.0	41.0	33.0	41.0
25	38.179173032051324	40.0	38.0	41.0	33.0	41.0
26	38.06395260427734	40.0	37.0	41.0	33.0	41.0
27	37.9915881252889	40.0	37.0	41.0	33.0	41.0
28	37.96535552330763	40.0	37.0	41.0	33.0	41.0
29	37.85950568025611	40.0	37.0	41.0	33.0	41.0
30	37.777403043904286	40.0	37.0	41.0	33.0	41.0
31	37.724525957174265	40.0	37.0	41.0	33.0	41.0
32	37.61523805111979	40.0	37.0	41.0	32.0	41.0
33	37.61269525408333	40.0	37.0	41.0	33.0	41.0
34	37.52650198740988	40.0	37.0	41.0	32.0	41.0
35	37.502197882983	40.0	37.0	41.0	32.0	41.0
36	37.46078805560115	40.0	36.0	41.0	32.0	41.0
37	37.386546438523226	39.0	36.0	41.0	32.0	41.0
38	37.33212957489469	39.0	36.0	41.0	31.0	41.0
39	37.23085575946046	39.0	36.0	41.0	31.0	41.0
40	37.10792939785559	39.0	36.0	41.0	31.0	41.0
41	37.04621445497034	39.0	36.0	41.0	31.0	41.0
42	36.9545015563932	39.0	36.0	41.0	31.0	41.0
43	36.269722789805854	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	12.0
11	9.0
12	11.0
13	8.0
14	7.0
15	8.0
16	12.0
17	14.0
18	17.0
19	47.0
20	106.0
21	237.0
22	432.0
23	742.0
24	1316.0
25	2293.0
26	3896.0
27	5862.0
28	8618.0
29	12342.0
30	16631.0
31	22044.0
32	28728.0
33	37309.0
34	49334.0
35	66618.0
36	91982.0
37	136461.0
38	232384.0
39	275512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.99633535112251	21.960098731018398	15.34590599606647	23.697659921792628
2	13.526371071126816	22.778627614675113	41.549504985392765	22.145496328805308
3	14.613580449566516	28.934210539566426	34.524235121616194	21.927973889250868
4	11.393342000662637	19.333213158899134	38.555046772359745	30.718398068078486
5	12.356583729522931	38.44799753474569	35.413861617460604	13.781557118270776
6	26.651634791908567	40.99220443886764	17.77107978743224	14.58508098179156
7	24.2975586127305	33.42816371786131	22.510249234391505	19.76402843501668
8	24.70329838539941	34.619300484088136	22.80974540811137	17.867655722401086
9	24.512966250789276	14.713781758527936	19.787492661135285	40.985759329547506
10	14.46262390470906	29.125348312841577	33.36713658898631	23.044891193463048
11	31.21990813705122	23.682554196823567	25.196248543556347	19.901289122568862
12	20.854158253617065	26.555864495604737	29.657069831752437	22.93290741902576
13	29.190101520542278	21.56090477250275	27.078723989200416	22.170269717754564
14	21.676916740265114	22.692927801683986	27.892519745700156	27.737635712350745
15	23.92746835098857	28.44408011673704	23.973792574227016	23.654658958047374
16	23.527972277973536	27.96734343671356	24.05234234406612	24.452341941246786
17	20.6156892087722	30.151731972072536	25.62575465684325	23.60682416231202
18	23.72897912489514	25.679933857065603	26.984564970226614	23.606522047812643
19	24.471475859540927	26.333508224060196	26.95868382811296	22.236332088285913
20	24.495544314658293	25.34710438358068	26.881342516271385	23.27600878548964
21	25.109340272567703	25.95173620167553	26.38194724879431	22.55697627696246
22	21.66301947329358	30.68224503298587	26.825048514553355	20.829686979167192
23	23.750328549518077	25.39886666780799	27.457474866591276	23.39332991608266
24	22.766543034699865	27.14649834189492	26.787989135962604	23.298969487442612
25	22.459594703328598	27.749619587492862	26.99876435169753	22.79202135748101
26	23.722634720408138	26.309339064109704	27.611754670941924	22.35627154454024
27	22.851638618692228	29.284159834682942	26.63411215094446	21.23008939568037
28	22.510249234391505	26.07681160441933	28.305812380853595	23.10712678033557
29	21.52565808090828	27.43985152079404	26.457173759140222	24.577316639157463
30	24.148213345203075	26.131695738473574	28.40712144297941	21.312969473343934
31	24.19232206211273	25.403398385298704	27.254756037506507	23.14952351508206
32	21.103906239772165	27.972277973536784	27.92555093096583	22.99826485572522
33	22.403904930609333	25.539752729352738	30.828770565185735	21.22757177485219
34	20.62404770992174	27.387787122067348	27.706215804415102	24.281949363595807
35	20.727270163876977	26.412762927731194	30.491207964543843	22.36875894384799
36	22.252545566419368	26.048211431811247	28.794532936019195	22.904710065750187
37	22.25868856124012	27.360596817123046	28.251028951632474	22.12968567000436
38	21.927571069918358	25.33350923110853	29.058581008478342	23.68033869049477
39	23.507126377516236	25.6825521827269	27.67298320948317	23.137338230273684
40	20.662516956176276	26.191917228683558	31.533603692242	21.611962122898166
41	22.29735921716091	25.871877269005772	27.451130462104267	24.37963305172905
42	22.819413072091567	25.333207116609145	29.701581367994596	22.14579844330469
43	22.64106481262355	24.778424190912194	29.967442127450024	22.61306886901423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	30.0
2	42.0
3	144.5
4	247.0
5	247.0
6	365.0
7	483.0
8	486.0
9	489.0
10	798.5
11	1108.0
12	1108.0
13	1681.5
14	2255.0
15	5431.5
16	8608.0
17	9535.0
18	10462.0
19	10462.0
20	10222.0
21	9982.0
22	11623.5
23	13265.0
24	16818.5
25	20372.0
26	20372.0
27	24320.0
28	28268.0
29	32909.5
30	37551.0
31	41848.0
32	46145.0
33	46145.0
34	49401.5
35	52658.0
36	54937.0
37	57216.0
38	58936.0
39	60656.0
40	60656.0
41	60889.5
42	61123.0
43	60957.5
44	60792.0
45	62369.0
46	63946.0
47	63946.0
48	65131.0
49	66316.0
50	65013.5
51	63711.0
52	66875.0
53	70039.0
54	70039.0
55	80100.5
56	90162.0
57	68722.0
58	47282.0
59	48734.0
60	50186.0
61	50186.0
62	41696.0
63	33206.0
64	24010.5
65	14815.0
66	12035.0
67	9255.0
68	9255.0
69	7386.0
70	5517.0
71	4511.5
72	3506.0
73	2641.5
74	1777.0
75	1777.0
76	1305.5
77	834.0
78	600.5
79	367.0
80	276.5
81	186.0
82	186.0
83	129.5
84	73.0
85	60.0
86	47.0
87	32.0
88	17.0
89	17.0
90	17.0
91	17.0
92	8.5
93	0.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	993001.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.98957376003666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.19929145686693	38.309499568087865
2	13.1824488718345	12.916051026892966
3	3.8507007904983106	5.659325712118454
4	1.5157267524396738	2.970192301548169
5	0.782405009785547	1.9164843968555632
6	0.4439241109136918	1.3048591785279002
7	0.3266658637668736	1.1202254999525338
8	0.20556454276269778	0.8056415464097114
9	0.1550354841379076	0.6835610057037317
>10	1.097275890031447	10.63604129033505
>50	0.13478444948314322	4.628632737931819
>100	0.09671140587936788	8.653633614928093
>500	0.005144223695625038	1.7059286075434796
>1k	0.0030865342173750226	2.731118818423181
>5k	8.230757913000061E-4	2.8335370341806816
>10k+	4.1153789565000307E-4	3.125267660560805
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT	17520	1.7643486763860259	No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG	13483	1.3578032650520997	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	9954	1.0024159089467182	Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	7225	0.7275924193429815	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5588	0.5627386075139904	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5342	0.5379652185647346	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	3787	0.3813692030521621	Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	3390	0.34138938430072074	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2929	0.2949644562291478	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1945	0.1958709004321244	No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG	1770	0.1782475546348896	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	1645	0.16565945049400757	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1601	0.16122843783641708	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1559	0.15699883484508073	No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA	1317	0.1326282652283331	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1298	0.13071487339891905	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1277	0.12860007190325085	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1219	0.12275919158188159	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1195	0.12034227558683223	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1084	0.10916403910972901	No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT	1077	0.1084591052778396	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	998	0.10050342346080215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0070483312705627E-4	0.0
5	0.0	0.0	0.0	1.0070483312705627E-4	0.0
6	0.0	0.0	0.0	1.0070483312705627E-4	0.0
7	0.0	0.0	0.0	1.0070483312705627E-4	0.0
8	0.0	0.0	0.0	1.0070483312705627E-4	0.0
9	0.0	0.0	0.0	1.0070483312705627E-4	0.0
10	0.0	0.0	0.0	3.021144993811688E-4	0.0
11	0.0	0.0	0.0	4.0281933250822506E-4	0.0
12	0.0	0.0	0.0	5.035241656352813E-4	0.0
13	0.0	0.0	0.0	5.035241656352813E-4	0.0
14	0.0	0.0	0.0	5.035241656352813E-4	0.0
15	0.0	0.0	0.0	6.042289987623376E-4	0.0
16	0.0	0.0	0.0	7.049338318893939E-4	0.0
17	0.0	0.0	0.0	7.049338318893939E-4	0.0
18	0.0	0.0	0.0	8.056386650164501E-4	0.0
19	0.0	0.0	0.0	9.063434981435064E-4	0.0
20	0.0	0.0	0.0	9.063434981435064E-4	0.0
21	0.0	0.0	0.0	0.0012084579975246753	0.0
22	0.0	0.0	0.0	0.0017119821631599566	0.0
23	0.0	0.0	0.0	0.0021148014956681818	0.0
24	0.0	0.0	0.0	0.002316211161922294	0.0
25	0.0	0.0	0.0	0.0025176208281764067	0.0
26	0.0	0.0	0.0	0.002618325661303463	0.0
27	0.0	0.0	0.0	0.0035246691594469693	0.0
28	0.0	0.0	0.0	0.011178236477103245	0.0
29	0.0	0.0	0.0	0.02729100977743225	0.0
30	0.0	0.0	0.0	0.0490432537328764	0.0
31	0.0	0.0	0.0	0.1004027186276751	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGC	20	0.0018418154	37.0	30
ATTACGG	65	0.0	37.0	2
CTTATAC	2145	0.0	32.256413	1
TCGCCGA	30	3.5991296E-4	30.833332	1
CATCGTA	30	3.5991296E-4	30.833332	9
CGTCTTC	520	0.0	30.240385	37
TAGACGA	25	0.0054959874	29.6	31
TTATACA	2370	0.0	29.116034	2
GATTACG	85	1.8189894E-12	28.294119	1
TATACAC	2485	0.0	28.21529	3
GGTATCA	730	0.0	27.369864	1
TTACGGG	90	5.456968E-12	26.722221	3
CGCCGTT	70	6.5901986E-9	26.428572	25
GTATCAA	2530	0.0	26.397234	1
AGGACCG	80	9.786163E-10	25.4375	5
TACGGCC	75	1.373337E-8	24.666666	19
CCGTCTT	1515	0.0	24.178215	37
GCCGTCT	1530	0.0	24.062092	36
GCCGTTT	90	3.8271537E-9	22.61111	26
CGTTACT	50	2.7017432E-4	22.2	27
>>END_MODULE