FastQCFastQC Report
Fri 10 Feb 2017
ERR1631081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742612
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT176112.371494131524942No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG136191.8339321206767463No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT104611.408676401674091RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC71520.9630870494955643No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC39240.5284051429279355RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC33530.4515143843622242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19050.2565269615896323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18350.2471007740246589No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG16320.21976483008623615No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA12840.17290321190608285No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC12760.17182593332722876No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT10590.14260475187581131No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT8750.11782734456216705RNA PCR Primer, Index 26 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8280.11149833291139923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC21250.033.6047061
CGTCTTC5300.031.06603837
TCCGGGT303.5982623E-430.83333413
TTATACA23500.030.2297882
TATACAC23950.029.8935283
GGTATCA2750.026.2363641
GTATCAA9200.025.135871
GATTACG601.3363424E-624.6666681
CGCAAGG451.3225965E-424.66666610
TGCGTTA451.3225965E-424.66666637
CCGTCTT15800.024.4715237
GCCGTCT16500.023.54545636
CGGCGTA400.001930547423.12512
GGACGTC502.7008538E-422.237
CGGACGT502.7008538E-422.236
CCGTCTA450.00382455320.5555552
TCGGCGT450.00382455320.55555511
CCGCAAG555.1407376E-420.1818189
ATTTCGG656.8979265E-519.92307729
TTAACGG851.2440942E-619.58823435
Produced by FastQC (version 0.11.5)