FastQCFastQC Report
Fri 10 Feb 2017
ERR1631080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007832
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT163821.6254693242524547No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG128201.27203740305924No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT88200.8751458576429404TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC69200.6866223735701982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34700.34430341564863987No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC31350.31106374872002474RNA PCR Primer, Index 12 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31000.3075909476976322No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC29290.29062383413108533No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG17290.1715563705061955No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16330.1620309734162043No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA11990.11896824073853579No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT11390.1130148675572913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11380.11291564467093723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC21300.031.8755871
CGTCTTC5300.030.71698237
TTATACA23000.030.0021722
GATTACG1050.029.952381
TATACAC23900.028.7175733
TTACGGG1150.027.3478263
GGTATCA6300.025.547621
GTATCAA17500.024.2085721
TGACGGA851.9754225E-923.94117521
CCGTCTT14650.023.48805437
GCCGTCT15050.022.86378936
GCGTAGC450.00382548620.55555524
TCGCCGA555.142491E-420.1818181
CGGTCCG555.142491E-420.18181821
GCAACGC656.901052E-519.9230773
AGCAACG701.2190078E-418.52
GCGGTAT609.235742E-418.56
TGGCGTA609.235742E-418.524
ATAGAAC902.1516807E-618.53
TAACGCA801.6163482E-518.515
Produced by FastQC (version 0.11.5)