##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631080.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1007832
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.558379769644148	31.0	31.0	34.0	30.0	34.0
2	31.558334127116424	31.0	31.0	34.0	30.0	34.0
3	31.019483405964486	31.0	31.0	34.0	27.0	34.0
4	35.163351630033574	37.0	35.0	37.0	32.0	37.0
5	35.36237884885576	37.0	35.0	37.0	33.0	37.0
6	35.56135149509045	37.0	35.0	37.0	33.0	37.0
7	35.57315802633772	37.0	35.0	37.0	33.0	37.0
8	35.592191952627026	37.0	35.0	37.0	33.0	37.0
9	37.38049496344629	39.0	37.0	39.0	34.0	39.0
10	37.21494653870883	39.0	37.0	39.0	34.0	39.0
11	37.271122568047055	39.0	37.0	39.0	34.0	39.0
12	37.21814746902262	39.0	37.0	39.0	34.0	39.0
13	37.276799109375375	39.0	37.0	39.0	34.0	39.0
14	38.43648941490248	40.0	38.0	41.0	34.0	41.0
15	38.44216000285762	40.0	38.0	41.0	34.0	41.0
16	38.43785869073417	40.0	38.0	41.0	34.0	41.0
17	38.422347176910435	40.0	38.0	41.0	34.0	41.0
18	38.458283721890155	40.0	38.0	41.0	34.0	41.0
19	38.48856456234769	40.0	38.0	41.0	34.0	41.0
20	38.47764806039102	40.0	38.0	41.0	34.0	41.0
21	38.42879368783686	40.0	38.0	41.0	34.0	41.0
22	38.34023230062153	40.0	38.0	41.0	34.0	41.0
23	38.33787674929949	40.0	38.0	41.0	34.0	41.0
24	38.28885568229626	40.0	38.0	41.0	34.0	41.0
25	38.23199104612674	40.0	38.0	41.0	34.0	41.0
26	38.156933893744196	40.0	38.0	41.0	33.0	41.0
27	38.078197556735645	40.0	37.0	41.0	33.0	41.0
28	38.057722914136484	40.0	37.0	41.0	33.0	41.0
29	37.96854733725462	40.0	37.0	41.0	33.0	41.0
30	37.86283527413299	40.0	37.0	41.0	33.0	41.0
31	37.85324240548028	40.0	37.0	41.0	33.0	41.0
32	37.710807952119005	40.0	37.0	41.0	33.0	41.0
33	37.70248712086935	40.0	37.0	41.0	33.0	41.0
34	37.67091340620262	40.0	37.0	41.0	33.0	41.0
35	37.59713622905405	40.0	37.0	41.0	32.0	41.0
36	37.59959695663563	40.0	37.0	41.0	32.0	41.0
37	37.5687336778352	40.0	37.0	41.0	32.0	41.0
38	37.52933623857945	40.0	37.0	41.0	32.0	41.0
39	37.436139158113654	40.0	37.0	41.0	32.0	41.0
40	37.36699370530009	40.0	36.0	41.0	31.0	41.0
41	37.31581553274752	39.0	36.0	41.0	31.0	41.0
42	37.25757963628859	39.0	36.0	41.0	31.0	41.0
43	36.590235277308125	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	5.0
10	20.0
11	14.0
12	11.0
13	7.0
14	9.0
15	9.0
16	9.0
17	10.0
18	24.0
19	36.0
20	69.0
21	189.0
22	370.0
23	697.0
24	1258.0
25	2056.0
26	3479.0
27	5407.0
28	7828.0
29	11025.0
30	15508.0
31	20745.0
32	27166.0
33	35994.0
34	47818.0
35	65105.0
36	91174.0
37	137871.0
38	242409.0
39	291504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.36671191230284	22.42963112899769	15.029489041824432	24.174167916875035
2	13.669837830114542	22.438958080314972	41.46931234570841	22.421891743862073
3	14.550242500734251	28.818195889791156	34.42289984838743	22.208661761087164
4	11.626044816993309	19.385572198541027	38.53142190365061	30.456961080815052
5	12.093979949039126	38.71329745433763	36.04102667904968	13.151695917573564
6	26.850109938958077	40.79340604386446	17.9640059057462	14.39247811143127
7	24.081196072361266	33.32539550242501	23.147409488883067	19.445998936330657
8	24.112748950221864	35.64592114558776	22.461382452630993	17.779947451559387
9	24.084569650497308	15.276752474618785	19.829197723430095	40.80948015145381
10	14.253566070535564	29.294068852745298	33.91289421252748	22.539470864191653
11	30.909417442589636	23.733221409917526	25.495519094452252	19.861842053040586
12	20.4319767580311	26.654839298613258	29.75168480461029	23.16149913874535
13	28.523702363092262	21.402674255233016	27.29155256034736	22.782070821327363
14	21.271600822359282	22.615971709570644	27.229042141944294	28.883385326125783
15	23.614650060724408	28.91831178212242	23.553131871184878	23.913906285968295
16	23.90080886496956	28.02183300391335	23.99169702886989	24.0856611022472
17	21.046960207653655	30.284114812786257	25.334281904126875	23.334643075433206
18	23.56880908722882	26.366299145095613	26.502532168059755	23.56235959961581
19	24.554092348724787	27.132399050635424	26.631720366092758	21.681788234547028
20	23.1786646980846	25.86274299684868	26.557203978440853	24.401388326625867
21	24.719596123163385	26.228577778836154	26.22133450813231	22.830491589868153
22	20.883540113828495	30.299692805943845	27.136566411862294	21.680200668365362
23	23.070610974845014	26.95816366219767	26.716258265266436	23.254967097690884
24	24.57115868517769	27.60936346533946	26.6180276077759	21.20145024170695
25	21.63922161630113	28.309579374340167	27.28004270553029	22.771156303828416
26	25.33586947030854	27.424808896720883	26.247628573016136	20.991693059954436
27	21.318830916263824	28.675017264782227	27.39166845267862	22.61448336627533
28	22.19219076195239	27.384425181974777	27.53117583089245	22.89220822518039
29	24.038331785456307	26.46691115185864	27.739643115122366	21.75511394756269
30	20.909040395621492	28.12333801665357	28.247664293255227	22.719957294469715
31	25.27951087085943	26.549960707737004	26.362528675414154	21.807999745989413
32	21.367450130577318	26.869061510251708	30.326284539486743	21.437203819684232
33	20.967581898570398	29.21885790488891	28.527968947205483	21.285591249335205
34	22.800923169734638	27.82289111677343	27.10789099770597	22.268294715785963
35	21.24034561315775	26.595007898141752	31.35016550377444	20.81448098492606
36	22.431020249406647	28.206982909850055	26.054937727716528	23.307059113026774
37	22.3969867993872	27.259106676509575	27.804336437025217	22.539570087078005
38	22.153295390501594	25.86691035807555	28.594150612403656	23.3856436390192
39	23.44487970217258	26.267473150286953	27.185384071948498	23.102263075591964
40	21.180811881345303	25.89300597718667	30.999412600512784	21.926769540955238
41	22.187924177839164	26.524162757284948	26.983862389763374	24.304050675112517
42	22.853412076615946	25.638896165233888	28.93279832353011	22.574893434620055
43	22.557330983735387	25.191202502004302	29.44042261011756	22.811043904142753
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	14.0
2	24.0
3	111.5
4	199.0
5	199.0
6	238.0
7	277.0
8	301.0
9	325.0
10	489.0
11	653.0
12	653.0
13	1032.5
14	1412.0
15	3451.5
16	5491.0
17	6267.5
18	7044.0
19	7044.0
20	7187.0
21	7330.0
22	9139.5
23	10949.0
24	13979.5
25	17010.0
26	17010.0
27	21377.0
28	25744.0
29	31744.0
30	37744.0
31	42376.0
32	47008.0
33	47008.0
34	51175.5
35	55343.0
36	58354.5
37	61366.0
38	65097.0
39	68828.0
40	68828.0
41	71150.5
42	73473.0
43	71657.0
44	69841.0
45	70964.5
46	72088.0
47	72088.0
48	72987.0
49	73886.0
50	86173.0
51	98460.0
52	83719.0
53	68978.0
54	68978.0
55	69914.5
56	70851.0
57	60265.5
58	49680.0
59	38788.5
60	27897.0
61	27897.0
62	24460.5
63	21024.0
64	17393.5
65	13763.0
66	11248.0
67	8733.0
68	8733.0
69	6965.0
70	5197.0
71	4328.0
72	3459.0
73	2652.0
74	1845.0
75	1845.0
76	1408.5
77	972.0
78	769.5
79	567.0
80	380.5
81	194.0
82	194.0
83	141.5
84	89.0
85	67.0
86	45.0
87	34.0
88	23.0
89	23.0
90	19.0
91	15.0
92	8.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1007832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.003461539732555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.44092552600813	39.4188380023423
2	11.560009555123235	11.329609672668447
3	3.254773728945764	4.784855377407771
4	1.3274696730618059	2.6020243627618225
5	0.7131572756722799	1.7473587565093505
6	0.4326340696781808	1.2720340196551625
7	0.31585787690236	1.0834690525964465
8	0.21823081949351175	0.8555252455867697
9	0.1759062710721532	0.7758014570173827
>10	1.2718326494148096	12.698439529333502
>50	0.17311998646694993	5.9358066273061
>100	0.10819542506334695	9.286043940659889
>500	0.00525809539833785	1.8266457644285967
>1k	0.0018201099455784863	1.9297117146784744
>5k	4.044688767952192E-4	1.5598635162817986
>10k+	4.044688767952192E-4	2.8939729607662694
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	16382	1.6254693242524547	No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	12820	1.27203740305924	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	8820	0.8751458576429404	TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	6920	0.6866223735701982	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3470	0.34430341564863987	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	3135	0.31106374872002474	RNA PCR Primer, Index 12 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3100	0.3075909476976322	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	2929	0.29062383413108533	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	1729	0.1715563705061955	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1633	0.1620309734162043	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	1199	0.11896824073853579	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	1139	0.1130148675572913	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1138	0.11291564467093723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.922288635407489E-5	0.0
6	0.0	0.0	0.0	9.922288635407489E-5	0.0
7	0.0	0.0	0.0	9.922288635407489E-5	0.0
8	0.0	0.0	0.0	9.922288635407489E-5	0.0
9	0.0	0.0	0.0	1.9844577270814977E-4	0.0
10	0.0	0.0	0.0	1.9844577270814977E-4	0.0
11	0.0	0.0	0.0	2.9766865906222467E-4	0.0
12	0.0	0.0	0.0	2.9766865906222467E-4	0.0
13	0.0	0.0	0.0	2.9766865906222467E-4	0.0
14	0.0	0.0	0.0	3.9689154541629955E-4	0.0
15	0.0	0.0	0.0	3.9689154541629955E-4	0.0
16	0.0	0.0	0.0	3.9689154541629955E-4	0.0
17	0.0	0.0	0.0	3.9689154541629955E-4	0.0
18	0.0	0.0	0.0	3.9689154541629955E-4	0.0
19	0.0	0.0	0.0	3.9689154541629955E-4	0.0
20	0.0	0.0	0.0	7.937830908325991E-4	0.0
21	0.0	0.0	0.0	0.0012898975226029736	0.0
22	0.0	0.0	0.0	0.0016867890680192731	0.0
23	0.0	0.0	0.0	0.0022821263861437222	0.0
24	0.0	0.0	0.0	0.002480572158851872	0.0
25	0.0	0.0	0.0	0.002480572158851872	0.0
26	0.0	0.0	0.0	0.0027782408179140967	0.0
27	0.0	0.0	0.0	0.003472801022392621	0.0
28	0.0	0.0	0.0	0.009128505544574889	0.0
29	0.0	0.0	0.0	0.022424372316020923	0.0
30	0.0	0.0	0.0	0.039689154541629953	0.0
31	0.0	0.0	0.0	0.08701847133252368	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2130	0.0	31.875587	1
CGTCTTC	530	0.0	30.716982	37
TTATACA	2300	0.0	30.002172	2
GATTACG	105	0.0	29.95238	1
TATACAC	2390	0.0	28.717573	3
TTACGGG	115	0.0	27.347826	3
GGTATCA	630	0.0	25.54762	1
GTATCAA	1750	0.0	24.208572	1
TGACGGA	85	1.9754225E-9	23.941175	21
CCGTCTT	1465	0.0	23.488054	37
GCCGTCT	1505	0.0	22.863789	36
GCGTAGC	45	0.003825486	20.555555	24
TCGCCGA	55	5.142491E-4	20.181818	1
CGGTCCG	55	5.142491E-4	20.181818	21
GCAACGC	65	6.901052E-5	19.923077	3
AGCAACG	70	1.2190078E-4	18.5	2
GCGGTAT	60	9.235742E-4	18.5	6
TGGCGTA	60	9.235742E-4	18.5	24
ATAGAAC	90	2.1516807E-6	18.5	3
TAACGCA	80	1.6163482E-5	18.5	15
>>END_MODULE