FastQCFastQC Report
Fri 10 Feb 2017
ERR1631079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800958
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT210542.6286022488070535No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG171962.1469290524596794No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT149001.860272323892139RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC90681.132144257251941No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC58350.7285026181148074RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC45160.5638248197783154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24910.31100257441713547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24820.309878919993308No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG21420.26742975287093707No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA16500.20600331103503552No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT13800.17229367832021156No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12700.1585601242512092No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT11290.14095620494457886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9450.117983714501884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAT200.00184160337.013
CTTATAC25750.033.3359221
TTATACA28150.030.756662
CGTCTTC6000.030.52537
CCGGCGT250.005495360629.623
TATACAC29450.029.5246163
GGTATCA3600.028.7777771
GATTACG405.9379156E-527.751
CCGTCTT22150.026.97742537
ACCGTTC358.8670396E-426.428578
GCCGTCT23250.025.70107736
TTACGGG451.3227228E-424.6666663
GTATCAA11500.023.6478271
TGCCGTC28050.021.3030335
CCGCGAT450.003824810320.55555532
ATGCCGT29800.020.05201534
GACCGTT656.898794E-519.9230777
TATGCCG32700.018.3868533
TACACAT51100.017.5949135
ATAACAC953.6036254E-617.5263163
Produced by FastQC (version 0.11.5)