FastQCFastQC Report
Fri 10 Feb 2017
ERR1631078.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631078.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043065
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT162201.555032524339327No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG110591.0602407328402352No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT74190.7112691922363419TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC55720.5341948967705752No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG51860.4971885740581843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28710.2752465090862027No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG27020.2590442589867362No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC26280.2519497826118219RNA PCR Primer, Index 11 (95% over 24bp)
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA26040.24964887135509292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24310.23306313604617163No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC24150.23152919520835233TruSeq Adapter, Index 11 (95% over 21bp)
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG20580.19730314026450893No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC19820.1900169212848672No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC16390.15713306457411572No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT15950.1529147272701126No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC14710.14102668577701294No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG14390.13795880410137432No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG14200.13613724935646387No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC13960.13383633809973491No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA13110.12568727739881982No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC13030.12492030697991018No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT12520.12003087055936111No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG12500.11983912795463371No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT10990.10536256129771394No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC10880.10430797697171318No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA10600.10162358050552937No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT10590.10152770920316567No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA10550.10114422399371084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG900.032.888891
CTTATAC19000.031.1578941
TTATACA20000.029.5999982
TATACAC21150.028.4278963
GGTATCA5850.028.1452981
CGTCTTC3850.025.94805137
CCGTCTT11250.025.48888837
GCCGTCT11450.025.04366936
GTATCAA16750.024.2985081
CCGGCGT652.682451E-622.7692323
GACGATT502.7018698E-422.214
CTGCGAC603.72586E-521.58333418
TGCCGTC13750.020.85454635
ACACCGT450.00382557520.5555556
TCGGCGT555.1426556E-420.18181811
ATCGGCG555.1426556E-420.18181810
GCGCTAT555.1426556E-420.18181819
GCGACTC656.901347E-519.92307720
GCGTCAA656.901347E-519.92307726
ATGCCGT14850.019.30976534
Produced by FastQC (version 0.11.5)