##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631078.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1043065
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.527182869715695	31.0	31.0	34.0	28.0	34.0
2	31.516086725180116	31.0	31.0	34.0	28.0	34.0
3	30.999860986611573	31.0	31.0	34.0	27.0	34.0
4	35.151515964968624	37.0	35.0	37.0	32.0	37.0
5	35.34552496728392	37.0	35.0	37.0	33.0	37.0
6	35.531279450465696	37.0	35.0	37.0	33.0	37.0
7	35.53474136319405	37.0	35.0	37.0	33.0	37.0
8	35.55271819110027	37.0	35.0	37.0	33.0	37.0
9	37.34826688653152	39.0	37.0	39.0	34.0	39.0
10	37.163169121770935	39.0	37.0	39.0	33.0	39.0
11	37.23174969920379	39.0	37.0	39.0	34.0	39.0
12	37.14721134349249	39.0	37.0	39.0	33.0	39.0
13	37.234947965850644	39.0	37.0	39.0	34.0	39.0
14	38.403177174960334	40.0	38.0	41.0	34.0	41.0
15	38.407969781365495	40.0	38.0	41.0	34.0	41.0
16	38.41415060422888	40.0	38.0	41.0	34.0	41.0
17	38.36934419235618	40.0	38.0	41.0	34.0	41.0
18	38.3960577720468	40.0	38.0	41.0	34.0	41.0
19	38.43994381941682	40.0	38.0	41.0	34.0	41.0
20	38.42046085335046	40.0	38.0	41.0	34.0	41.0
21	38.37944806891229	40.0	38.0	41.0	34.0	41.0
22	38.30790698566245	40.0	38.0	41.0	34.0	41.0
23	38.30692813966532	40.0	38.0	41.0	34.0	41.0
24	38.280514637151086	40.0	38.0	41.0	34.0	41.0
25	38.23953924252084	40.0	38.0	41.0	34.0	41.0
26	38.15917991687958	40.0	38.0	41.0	33.0	41.0
27	38.074111392866214	40.0	37.0	41.0	33.0	41.0
28	38.046921332802846	40.0	37.0	41.0	33.0	41.0
29	37.96753030731546	40.0	37.0	41.0	33.0	41.0
30	37.887266852976566	40.0	37.0	41.0	33.0	41.0
31	37.86085526788839	40.0	37.0	41.0	33.0	41.0
32	37.765779697334295	40.0	37.0	41.0	33.0	41.0
33	37.7700871949495	40.0	37.0	41.0	33.0	41.0
34	37.72732475924319	40.0	37.0	41.0	33.0	41.0
35	37.70544596933077	40.0	37.0	41.0	33.0	41.0
36	37.69180348300441	40.0	37.0	41.0	33.0	41.0
37	37.63720381759526	40.0	37.0	41.0	33.0	41.0
38	37.61677172563551	40.0	37.0	41.0	33.0	41.0
39	37.5355303840125	40.0	37.0	41.0	32.0	41.0
40	37.45169093009544	39.0	37.0	41.0	32.0	41.0
41	37.410707865761005	39.0	36.0	41.0	32.0	41.0
42	37.35382071107745	39.0	36.0	41.0	32.0	41.0
43	36.70951282997704	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	13.0
11	13.0
12	17.0
13	9.0
14	7.0
15	3.0
16	7.0
17	15.0
18	22.0
19	30.0
20	65.0
21	148.0
22	326.0
23	595.0
24	1085.0
25	2069.0
26	3439.0
27	5314.0
28	7821.0
29	11611.0
30	16041.0
31	21715.0
32	28577.0
33	37593.0
34	50106.0
35	67537.0
36	95476.0
37	142181.0
38	248777.0
39	302446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.253291980844914	21.7891502447115	14.303135470943806	23.654422303499782
2	14.33506061463092	22.384127547180665	40.97338133289872	22.307430505289698
3	14.919396202537714	27.888290758485805	33.51584033593304	23.676472703043437
4	11.334959949763437	19.171000848461027	39.07484193219023	30.419197269585307
5	12.545239270802874	37.7940013326111	36.52734968578181	13.133409710804218
6	28.240617794672428	40.552410444219674	17.511468604545257	13.69550315656263
7	26.136530321696156	32.22397453658209	22.840666689036638	18.798828452685115
8	24.709198372105288	35.41390038012972	21.814939625047337	18.061961622717664
9	24.307018258689535	14.773192466433061	20.02943248982566	40.89035678505174
10	14.323460187044912	29.220614247434245	34.34666104221693	22.109264523303917
11	32.03664201176341	23.829003945104095	24.935167031776544	19.199187011355956
12	21.120735524631733	25.674430644303087	30.233686299511536	22.971147531553644
13	28.289608030180286	21.371343109010464	27.30721479485938	23.03183406594987
14	21.77544064847349	21.881378437585386	26.73026129723459	29.612919616706534
15	23.013330904593673	29.391552779548736	23.95852607459746	23.636590241260134
16	24.906597383672157	27.863172477266517	23.596324294267376	23.633905844793947
17	22.238882524099647	29.29942045797721	25.159889364517074	23.30180765340607
18	23.77234400540714	25.916122197561993	26.87541044901324	23.43612334801762
19	25.546346584345176	26.860071040635052	26.127710161878692	21.46587221314108
20	24.063121665476263	27.045102654197006	25.75266162703187	23.139114053294858
21	24.11757656521885	27.182102745274744	25.506751736468964	23.193568953037445
22	23.50342500227694	28.40187332524819	26.23115529712913	21.863546375345734
23	22.49390018838711	26.214665433122576	26.997742230829335	24.29369214766098
24	23.723833126411105	28.435428281075488	26.285322582964625	21.555416009548782
25	22.462933757723633	29.026091374938286	26.291650088920633	22.21932477841745
26	25.685264101470185	26.71482601755403	25.360548000364314	22.239361880611465
27	23.471691601194557	28.55766419158921	25.250200131343686	22.72044407587255
28	22.071299487567888	26.618858843887967	27.378255429910887	23.93158623863326
29	25.331690738352837	26.796795981075007	26.75653003408225	21.114983246489913
30	23.804748505606074	26.813573458988653	26.331244936796843	23.050433098608426
31	22.342231788047727	26.849621068677408	27.322170718028122	23.48597642524675
32	23.345524967283918	27.813798756549208	26.78433271176772	22.05634356439915
33	21.78138466922004	25.489686644648224	27.943033272135487	24.78589541399625
34	22.65601856068414	27.168105535129644	28.352978961042695	21.822896943143526
35	22.188550090358703	28.0655567965563	27.920791129987105	21.825101983097888
36	22.804043851533702	25.960414739254023	25.111570228125764	26.123971181086507
37	22.49188689103747	27.15612162233418	27.8130317861303	22.53895970049805
38	22.133040606290116	25.240037773293132	27.931720458456567	24.695201161960185
39	23.564207407975534	25.923696030448724	27.082396590816487	23.429699970759252
40	22.191713843336704	25.76646709457225	29.93888204474314	22.102937017347912
41	22.720060590663095	26.36317008048396	26.205749402002752	24.7110199268502
42	22.58449856912081	27.205974699563306	27.875635746573803	22.333890984742084
43	23.858340563627387	24.529439680173333	28.12125802322962	23.490961732969662
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	17.0
2	22.0
3	106.0
4	190.0
5	190.0
6	253.5
7	317.0
8	301.0
9	285.0
10	437.5
11	590.0
12	590.0
13	896.5
14	1203.0
15	2728.0
16	4253.0
17	5028.0
18	5803.0
19	5803.0
20	6179.0
21	6555.0
22	8615.5
23	10676.0
24	14011.5
25	17347.0
26	17347.0
27	21076.5
28	24806.0
29	29295.0
30	33784.0
31	38584.5
32	43385.0
33	43385.0
34	54430.5
35	65476.0
36	67044.5
37	68613.0
38	69242.0
39	69871.0
40	69871.0
41	69626.0
42	69381.0
43	71779.5
44	74178.0
45	72467.5
46	70757.0
47	70757.0
48	67429.0
49	64101.0
50	66355.0
51	68609.0
52	82107.0
53	95605.0
54	95605.0
55	80620.0
56	65635.0
57	62074.5
58	58514.0
59	51392.5
60	44271.0
61	44271.0
62	36507.0
63	28743.0
64	21917.0
65	15091.0
66	12729.0
67	10367.0
68	10367.0
69	8825.5
70	7284.0
71	7947.5
72	8611.0
73	7778.5
74	6946.0
75	6946.0
76	3952.5
77	959.0
78	696.0
79	433.0
80	318.0
81	203.0
82	203.0
83	148.5
84	94.0
85	74.0
86	54.0
87	40.5
88	27.0
89	27.0
90	20.5
91	14.0
92	7.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1043065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.667187650030954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.5749903792334	41.01267542364885
2	10.710037951676107	10.638749293697005
3	2.6913338576737953	4.010129512138983
4	1.0715897709665423	2.128914009537958
5	0.5866880180681615	1.4569571942708062
6	0.38651109872530903	1.1518151561525736
7	0.24995223151026572	0.8690097070175042
8	0.183768965640274	0.7301830160566064
9	0.15576545547468848	0.6962788895808504
>10	1.1399928782934676	11.430000831217995
>50	0.13727201789191795	4.858464123158351
>100	0.09757698957245214	9.649574234164623
>500	0.008905836301190243	3.133700449442236
>1k	0.004646523287577519	3.8625798077156457
>5k	5.808154109471898E-4	1.747868086455906
>10k+	3.8721027396479326E-4	2.6231002657441085
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	16220	1.555032524339327	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	11059	1.0602407328402352	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	7419	0.7112691922363419	TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	5572	0.5341948967705752	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	5186	0.4971885740581843	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2871	0.2752465090862027	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	2702	0.2590442589867362	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	2628	0.2519497826118219	RNA PCR Primer, Index 11 (95% over 24bp)
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	2604	0.24964887135509292	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2431	0.23306313604617163	No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	2415	0.23152919520835233	TruSeq Adapter, Index 11 (95% over 21bp)
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	2058	0.19730314026450893	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1982	0.1900169212848672	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1639	0.15713306457411572	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	1595	0.1529147272701126	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	1471	0.14102668577701294	No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG	1439	0.13795880410137432	No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	1420	0.13613724935646387	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	1396	0.13383633809973491	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	1311	0.12568727739881982	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	1303	0.12492030697991018	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	1252	0.12003087055936111	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	1250	0.11983912795463371	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	1099	0.10536256129771394	No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC	1088	0.10430797697171318	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	1060	0.10162358050552937	No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT	1059	0.10152770920316567	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	1055	0.10114422399371084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.587130236370697E-5	0.0
8	0.0	0.0	0.0	9.587130236370697E-5	0.0
9	0.0	0.0	0.0	1.9174260472741393E-4	0.0
10	0.0	0.0	0.0	1.9174260472741393E-4	0.0
11	0.0	0.0	0.0	2.876139070911209E-4	0.0
12	0.0	0.0	0.0	2.876139070911209E-4	0.0
13	0.0	0.0	0.0	2.876139070911209E-4	0.0
14	0.0	0.0	0.0	2.876139070911209E-4	0.0
15	0.0	0.0	0.0	2.876139070911209E-4	0.0
16	9.587130236370697E-5	0.0	0.0	2.876139070911209E-4	0.0
17	9.587130236370697E-5	0.0	0.0	3.8348520945482786E-4	0.0
18	1.9174260472741393E-4	0.0	0.0	4.793565118185348E-4	0.0
19	1.9174260472741393E-4	0.0	0.0	4.793565118185348E-4	0.0
20	1.9174260472741393E-4	0.0	0.0	5.752278141822418E-4	0.0
21	2.876139070911209E-4	0.0	0.0	7.669704189096557E-4	0.0
22	2.876139070911209E-4	0.0	0.0	0.0012463269307281906	0.0
23	3.8348520945482786E-4	0.0	0.0	0.0016298121401830184	0.0
24	3.8348520945482786E-4	0.0	0.0	0.0020132973496378463	0.0
25	3.8348520945482786E-4	0.0	0.0	0.002492653861456381	0.0
26	3.8348520945482786E-4	0.0	0.0	0.002780267768547502	0.0
27	3.8348520945482786E-4	0.0	0.0	0.003355495582729744	0.0
28	3.8348520945482786E-4	0.0	0.0	0.008340803305642505	0.0
29	3.8348520945482786E-4	0.0	0.0	0.018503161356195442	0.0
30	3.8348520945482786E-4	0.0	0.0	0.03489715406038933	0.0
31	3.8348520945482786E-4	0.0	0.0	0.07928556705478565	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	90	0.0	32.88889	1
CTTATAC	1900	0.0	31.157894	1
TTATACA	2000	0.0	29.599998	2
TATACAC	2115	0.0	28.427896	3
GGTATCA	585	0.0	28.145298	1
CGTCTTC	385	0.0	25.948051	37
CCGTCTT	1125	0.0	25.488888	37
GCCGTCT	1145	0.0	25.043669	36
GTATCAA	1675	0.0	24.298508	1
CCGGCGT	65	2.682451E-6	22.76923	23
GACGATT	50	2.7018698E-4	22.2	14
CTGCGAC	60	3.72586E-5	21.583334	18
TGCCGTC	1375	0.0	20.854546	35
ACACCGT	45	0.003825575	20.555555	6
TCGGCGT	55	5.1426556E-4	20.181818	11
ATCGGCG	55	5.1426556E-4	20.181818	10
GCGCTAT	55	5.1426556E-4	20.181818	19
GCGACTC	65	6.901347E-5	19.923077	20
GCGTCAA	65	6.901347E-5	19.923077	26
ATGCCGT	1485	0.0	19.309765	34
>>END_MODULE