FastQCFastQC Report
Fri 10 Feb 2017
ERR1631077.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631077.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772350
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT175352.2703437560691397No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG137251.7770440862303358No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT113591.4707062860102285TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC72290.9359746229041238No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC41180.5331779633585809RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC33470.43335275458017736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22990.2976629766297663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22090.2860102285233379No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG16800.21751796465333076No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA12830.16611639800608533No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10880.14086877710882373No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT10450.13530135301353013No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT8360.10824108241082411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7890.10215575839968927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20500.034.653661
CGTCTTC4900.032.4693937
TTATACA22450.031.7260592
TATACAC23800.030.1596623
CCGTCTT16500.026.57272537
GCCGTCT17300.025.3439336
GTATCAA11100.024.01
GGTATCA3500.023.7857151
GATTACG806.954033E-720.81251
TGCCGTC21500.020.56511535
CCGTTAA450.003824688520.55555516
ATGCCGT22400.019.49107234
TACACAT39750.018.7559765
GCGAACT801.615552E-518.528
GACCGTG801.615552E-518.57
TTAACGG902.1503565E-618.535
GGACCGT801.615552E-518.56
TGGACCG1002.8718932E-718.4999985
CGCCGAG500.007032844218.4999982
ATACACA39700.018.4068014
Produced by FastQC (version 0.11.5)