##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631077.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	772350
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42554023434971	31.0	31.0	34.0	28.0	34.0
2	31.433654431281155	31.0	31.0	34.0	28.0	34.0
3	30.89775101961546	31.0	31.0	34.0	27.0	34.0
4	35.04975335016508	37.0	35.0	37.0	32.0	37.0
5	35.23260956820094	37.0	35.0	37.0	32.0	37.0
6	35.41862368097365	37.0	35.0	37.0	33.0	37.0
7	35.428210008415874	37.0	35.0	37.0	33.0	37.0
8	35.447912215964266	37.0	35.0	37.0	33.0	37.0
9	37.226552728685185	39.0	37.0	39.0	34.0	39.0
10	37.03676959927494	39.0	37.0	39.0	33.0	39.0
11	37.10289117628018	39.0	37.0	39.0	33.0	39.0
12	37.02637923221337	39.0	37.0	39.0	33.0	39.0
13	37.11554476597397	39.0	37.0	39.0	33.0	39.0
14	38.225336958632745	40.0	38.0	41.0	33.0	41.0
15	38.22905677477827	40.0	38.0	41.0	33.0	41.0
16	38.21996374700589	40.0	38.0	41.0	33.0	41.0
17	38.183147536738524	40.0	38.0	41.0	33.0	41.0
18	38.20973133941866	40.0	38.0	41.0	33.0	41.0
19	38.2247193629831	40.0	38.0	41.0	34.0	41.0
20	38.21685116851169	40.0	38.0	41.0	34.0	41.0
21	38.16488638570596	40.0	38.0	41.0	33.0	41.0
22	38.09272997993138	40.0	37.0	41.0	33.0	41.0
23	38.11679678902052	40.0	37.0	41.0	33.0	41.0
24	38.06605425001619	40.0	37.0	41.0	33.0	41.0
25	38.024488897520555	40.0	37.0	41.0	33.0	41.0
26	37.8956159772124	40.0	37.0	41.0	33.0	41.0
27	37.793097688871626	40.0	37.0	41.0	33.0	41.0
28	37.80197708292872	40.0	37.0	41.0	33.0	41.0
29	37.704947238946076	40.0	37.0	41.0	33.0	41.0
30	37.616335858095425	40.0	37.0	41.0	32.0	41.0
31	37.60238363436266	39.0	37.0	41.0	32.0	41.0
32	37.51405450896614	39.0	37.0	41.0	32.0	41.0
33	37.51569884119894	39.0	37.0	41.0	32.0	41.0
34	37.44665760341814	39.0	36.0	41.0	32.0	41.0
35	37.43064025377096	39.0	36.0	41.0	32.0	41.0
36	37.41885673593578	39.0	37.0	41.0	32.0	41.0
37	37.33817181329708	39.0	36.0	41.0	31.0	41.0
38	37.296486049071014	39.0	36.0	41.0	31.0	41.0
39	37.222502751343306	39.0	36.0	41.0	31.0	41.0
40	37.12705250210397	39.0	36.0	41.0	31.0	41.0
41	37.088077943937336	39.0	36.0	41.0	31.0	41.0
42	37.02657991843076	39.0	36.0	41.0	31.0	41.0
43	36.31719168770635	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	16.0
11	16.0
12	17.0
13	8.0
14	6.0
15	13.0
16	10.0
17	20.0
18	22.0
19	31.0
20	79.0
21	141.0
22	309.0
23	623.0
24	1068.0
25	1829.0
26	3006.0
27	4733.0
28	6807.0
29	9873.0
30	13422.0
31	17655.0
32	23189.0
33	30133.0
34	39713.0
35	53826.0
36	73778.0
37	110712.0
38	190034.0
39	191255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.21007315336311	22.263481582184244	15.129928141386678	25.396517123065966
2	13.945102608920825	21.17563280895967	41.31999741050042	23.559267171619084
3	14.484495371269501	28.735547355473557	33.84799637470059	22.93196089855635
4	11.496860231760213	19.48779698323299	37.273645368032625	31.74169741697417
5	12.524244189810318	38.037677218877455	35.71670874603483	13.721369845277401
6	27.779763060788504	39.85045639930084	17.599404415096785	14.77037612481388
7	24.529811613905615	32.68751213827928	22.741373729526767	20.04130251828834
8	24.73347575581019	35.06247167734835	22.309963099631	17.89408946721046
9	23.636693209037354	14.554800284844955	19.17666860879135	42.63183789732634
10	14.522690490062795	28.43387065449602	33.503204505729265	23.54023434971192
11	31.06467275199068	22.751213827927753	25.456075613387714	20.728037806693855
12	20.361882566194083	26.28393862885997	28.427267430569042	24.9269113743769
13	29.098465721499323	20.019550721823006	26.755486502233445	24.12649705444423
14	21.711141321939532	22.136725577782094	25.932543535961678	30.219589564316696
15	24.214410565158285	28.375736388942833	22.190457694050625	25.219395351848256
16	25.326212209490517	26.427396905548	23.149349388230725	25.097041496730753
17	20.84042208843141	30.721434582766882	24.499126043892016	23.939017284909692
18	24.088819835566778	25.030750307503073	25.086165598498088	25.794264258432058
19	23.83634362659416	26.771152974687638	25.05703372823202	24.33546967048618
20	23.581536868000256	25.53427850067974	25.57130834466239	25.312876286657605
21	24.19628406810384	25.53427850067974	26.57797630607885	23.691461125137568
22	21.560044021492846	31.238428173755423	25.288146565676183	21.913381239075548
23	23.067780151485724	24.56943095746747	26.455751925940312	25.907036965106496
24	23.34524503139768	27.96892600504952	26.47996374700589	22.2058652165469
25	22.978183466045188	28.772706674435167	25.521201527804756	22.727908331714897
26	25.075419175244384	25.595779115685897	26.685570013594873	22.64323169547485
27	22.998122612805076	29.932543535961674	25.40519194665631	21.66414190457694
28	21.267560044021494	24.68479316372111	29.222502751343306	24.825144040914093
29	21.708033922444486	26.915129151291513	26.70499126043892	24.671845665825078
30	23.651194406680908	25.813038130381305	27.0737360005179	23.462031462419887
31	23.11089531947951	24.530070563863536	29.579465268336897	22.77956884832006
32	21.97863662847155	29.80669385641225	26.633650547031785	21.581018968084418
33	22.691655337606008	25.37696640124296	27.928529811613906	24.002848449537126
34	21.518870978183465	27.693532724800935	28.563734058393216	22.223862238622385
35	21.86107334757558	27.275587492717037	30.091279860167024	20.772059299540363
36	25.69806434906454	25.36557260309445	24.619667249304072	24.316695798536934
37	22.38285751278565	26.564640383245937	27.71411924645562	23.338382857512784
38	22.441768628212596	24.690231112837445	28.210396840810514	24.657603418139445
39	24.24108241082411	25.250598821777693	26.46313199974105	24.04518676765715
40	21.33954813232343	24.26723635657409	32.1487667508254	22.244448760277077
41	22.598821777691462	25.570919919725515	26.083770311387322	25.7464879911957
42	23.065579076843402	24.749401178222307	29.50501715543471	22.68000258949958
43	23.376836926263998	23.960510131417102	29.651841781575712	23.010811160743184
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	11.5
2	21.0
3	61.5
4	102.0
5	102.0
6	157.0
7	212.0
8	210.5
9	209.0
10	346.5
11	484.0
12	484.0
13	712.5
14	941.0
15	2304.5
16	3668.0
17	4076.5
18	4485.0
19	4485.0
20	4422.5
21	4360.0
22	5102.5
23	5845.0
24	7790.5
25	9736.0
26	9736.0
27	12339.0
28	14942.0
29	18010.5
30	21079.0
31	24796.5
32	28514.0
33	28514.0
34	33066.5
35	37619.0
36	40997.0
37	44375.0
38	47526.0
39	50677.0
40	50677.0
41	53072.5
42	55468.0
43	56970.0
44	58472.0
45	58832.0
46	59192.0
47	59192.0
48	59298.0
49	59404.0
50	72233.5
51	85063.0
52	66807.0
53	48551.0
54	48551.0
55	55887.0
56	63223.0
57	52459.5
58	41696.0
59	32548.5
60	23401.0
61	23401.0
62	20707.5
63	18014.0
64	14510.5
65	11007.0
66	9203.0
67	7399.0
68	7399.0
69	6099.0
70	4799.0
71	3984.5
72	3170.0
73	3013.5
74	2857.0
75	2857.0
76	2415.5
77	1974.0
78	1427.0
79	880.0
80	579.0
81	278.0
82	278.0
83	196.0
84	114.0
85	89.0
86	64.0
87	49.0
88	34.0
89	34.0
90	24.5
91	15.0
92	9.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	772350.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.80022651001859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.51287936503292	40.59359855862459
2	10.74174179080375	10.698823485883189
3	2.9676971536992767	4.433759714020844
4	1.231942670770147	2.454040962068423
5	0.6870779561894098	1.7108318924136616
6	0.4433599555341036	1.3247655726642078
7	0.3179363086465563	1.1083310130450381
8	0.22917610175575853	0.9130417422495877
9	0.18254147192944695	0.8181545984602792
>10	1.43113973170693	14.329337762784819
>50	0.17192940115604446	5.87737681928382
>100	0.0773681184681408	6.370340105225554
>500	0.002083989723036735	0.6865256050655266
>1k	0.002083989723036735	2.2143434529220474
>5k	2.604987153795919E-4	0.9378105818251498
>10k+	7.814961461387756E-4	5.52891813346328
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	17535	2.2703437560691397	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	13725	1.7770440862303358	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	11359	1.4707062860102285	TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	7229	0.9359746229041238	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	4118	0.5331779633585809	RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	3347	0.43335275458017736	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2299	0.2976629766297663	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2209	0.2860102285233379	No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG	1680	0.21751796465333076	No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	1283	0.16611639800608533	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1088	0.14086877710882373	No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT	1045	0.13530135301353013	No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	836	0.10824108241082411	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	789	0.10215575839968927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2947497896031592E-4
2	0.0	0.0	0.0	0.0	1.2947497896031592E-4
3	0.0	0.0	0.0	0.0	1.2947497896031592E-4
4	0.0	0.0	0.0	0.0	1.2947497896031592E-4
5	0.0	0.0	0.0	0.0	1.2947497896031592E-4
6	0.0	0.0	0.0	0.0	1.2947497896031592E-4
7	0.0	0.0	0.0	0.0	1.2947497896031592E-4
8	0.0	0.0	0.0	1.2947497896031592E-4	1.2947497896031592E-4
9	0.0	0.0	0.0	2.5894995792063183E-4	1.2947497896031592E-4
10	0.0	0.0	0.0	2.5894995792063183E-4	1.2947497896031592E-4
11	0.0	0.0	0.0	3.884249368809478E-4	1.2947497896031592E-4
12	0.0	0.0	0.0	3.884249368809478E-4	1.2947497896031592E-4
13	0.0	0.0	0.0	3.884249368809478E-4	1.2947497896031592E-4
14	0.0	0.0	0.0	3.884249368809478E-4	1.2947497896031592E-4
15	0.0	0.0	0.0	3.884249368809478E-4	1.2947497896031592E-4
16	0.0	0.0	0.0	5.178999158412637E-4	2.5894995792063183E-4
17	0.0	0.0	0.0	5.178999158412637E-4	2.5894995792063183E-4
18	0.0	0.0	0.0	5.178999158412637E-4	3.884249368809478E-4
19	0.0	0.0	0.0	6.473748948015796E-4	3.884249368809478E-4
20	0.0	0.0	0.0	7.768498737618955E-4	5.178999158412637E-4
21	0.0	0.0	0.0	7.768498737618955E-4	5.178999158412637E-4
22	0.0	0.0	0.0	0.001553699747523791	5.178999158412637E-4
23	0.0	1.2947497896031592E-4	0.0	0.0018126497054444229	5.178999158412637E-4
24	0.0	1.2947497896031592E-4	0.0	0.0020715996633650547	5.178999158412637E-4
25	0.0	1.2947497896031592E-4	0.0	0.0023305496212856864	5.178999158412637E-4
26	0.0	1.2947497896031592E-4	0.0	0.0024600246002460025	5.178999158412637E-4
27	0.0	1.2947497896031592E-4	0.0	0.003236874474007898	5.178999158412637E-4
28	0.0	1.2947497896031592E-4	0.0	0.008286398653460219	5.178999158412637E-4
29	0.0	1.2947497896031592E-4	0.0	0.01929177186508707	5.178999158412637E-4
30	0.0	1.2947497896031592E-4	0.0	0.03482876934032498	5.178999158412637E-4
31	0.0	1.2947497896031592E-4	0.0	0.07276493817569754	5.178999158412637E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2050	0.0	34.65366	1
CGTCTTC	490	0.0	32.46939	37
TTATACA	2245	0.0	31.726059	2
TATACAC	2380	0.0	30.159662	3
CCGTCTT	1650	0.0	26.572725	37
GCCGTCT	1730	0.0	25.34393	36
GTATCAA	1110	0.0	24.0	1
GGTATCA	350	0.0	23.785715	1
GATTACG	80	6.954033E-7	20.8125	1
TGCCGTC	2150	0.0	20.565115	35
CCGTTAA	45	0.0038246885	20.555555	16
ATGCCGT	2240	0.0	19.491072	34
TACACAT	3975	0.0	18.755976	5
GCGAACT	80	1.615552E-5	18.5	28
GACCGTG	80	1.615552E-5	18.5	7
TTAACGG	90	2.1503565E-6	18.5	35
GGACCGT	80	1.615552E-5	18.5	6
TGGACCG	100	2.8718932E-7	18.499998	5
CGCCGAG	50	0.0070328442	18.499998	2
ATACACA	3970	0.0	18.406801	4
>>END_MODULE