FastQCFastQC Report
Fri 10 Feb 2017
ERR1631076.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631076.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200790
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT41110.34235794768444106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36150.301051807560023No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG34700.2889764238542959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33560.279482673906345No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC19830.165141281989357No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT19820.16505800348104166No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16390.1364934751288735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGC509.091531E-625.8999983
GGTATCA6850.024.5766431
GTATCAA17800.023.5926971
CTTATAC8200.021.4329261
GATTACG450.003825905320.5555571
TATACAC9700.020.2164963
CGAACTA851.2454038E-619.58823424
TTATACA10650.018.4131452
GGTTCGC1156.405571E-817.69565223
TTCGGAC650.001579989617.0769233
GCCGTCT3800.017.03947436
CGGTTCG1201.0412805E-716.95833222
CCGTCTT3850.016.81818237
GACGTTA1251.6577724E-716.27999920
TGCGTTA700.002592584815.85714237
CGTTACC1455.3452823E-815.31034422
TTCGCAC855.3643994E-415.23529327
ACCCGCA1601.0963049E-815.0312526
CGTCTTC2350.014.95744637
ACAATCG750.004104546714.837
Produced by FastQC (version 0.11.5)