##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631076.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1200790
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.631062050816546	31.0	31.0	34.0	30.0	34.0
2	31.61424395606226	33.0	31.0	34.0	30.0	34.0
3	31.086004213892522	31.0	31.0	34.0	27.0	34.0
4	35.226418441192884	37.0	35.0	37.0	32.0	37.0
5	35.430638163209224	37.0	35.0	37.0	33.0	37.0
6	35.61810891163317	37.0	35.0	37.0	33.0	37.0
7	35.632078881403075	37.0	35.0	37.0	33.0	37.0
8	35.64617043779512	37.0	35.0	37.0	33.0	37.0
9	37.41897417533457	39.0	37.0	39.0	34.0	39.0
10	37.274976473821404	39.0	37.0	39.0	34.0	39.0
11	37.335080238842764	39.0	37.0	39.0	34.0	39.0
12	37.28286877805445	39.0	37.0	39.0	34.0	39.0
13	37.34067405624631	39.0	37.0	39.0	34.0	39.0
14	38.557373895518786	40.0	38.0	41.0	34.0	41.0
15	38.56146287027707	40.0	38.0	41.0	34.0	41.0
16	38.5738988499238	40.0	38.0	41.0	34.0	41.0
17	38.553020927889136	40.0	38.0	41.0	34.0	41.0
18	38.56877722166241	40.0	38.0	41.0	34.0	41.0
19	38.606769709940956	40.0	38.0	41.0	34.0	41.0
20	38.59509656143039	40.0	38.0	41.0	34.0	41.0
21	38.56287110985268	40.0	38.0	41.0	34.0	41.0
22	38.509656143039166	40.0	38.0	41.0	34.0	41.0
23	38.48620075117214	40.0	38.0	41.0	34.0	41.0
24	38.46357564603303	40.0	38.0	41.0	34.0	41.0
25	38.43508606833834	40.0	38.0	41.0	34.0	41.0
26	38.34133612038741	40.0	38.0	41.0	34.0	41.0
27	38.289430291724614	40.0	38.0	41.0	34.0	41.0
28	38.25133287252559	40.0	38.0	41.0	34.0	41.0
29	38.18071436304433	40.0	38.0	41.0	34.0	41.0
30	38.100436379383574	40.0	38.0	41.0	33.0	41.0
31	38.07461254674006	40.0	37.0	41.0	33.0	41.0
32	37.997392549904646	40.0	37.0	41.0	33.0	41.0
33	37.98827771716953	40.0	37.0	41.0	33.0	41.0
34	37.92328300535481	40.0	37.0	41.0	33.0	41.0
35	37.881091614686994	40.0	37.0	41.0	33.0	41.0
36	37.84786515543934	40.0	37.0	41.0	33.0	41.0
37	37.80604518691861	40.0	37.0	41.0	33.0	41.0
38	37.76930187626479	40.0	37.0	41.0	33.0	41.0
39	37.70637163867121	40.0	37.0	41.0	33.0	41.0
40	37.668739746333664	40.0	37.0	41.0	33.0	41.0
41	37.602140257663706	40.0	37.0	41.0	32.0	41.0
42	37.53223960892412	40.0	37.0	41.0	32.0	41.0
43	36.97068679785808	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	7.0
11	7.0
12	7.0
13	6.0
14	3.0
15	3.0
16	4.0
17	8.0
18	14.0
19	38.0
20	78.0
21	158.0
22	301.0
23	629.0
24	1170.0
25	2028.0
26	3278.0
27	5303.0
28	7981.0
29	11542.0
30	16576.0
31	22658.0
32	30126.0
33	39965.0
34	53942.0
35	73667.0
36	105029.0
37	158932.0
38	285972.0
39	381352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.49041880761832	22.084544341641752	14.833401344115124	23.591635506624804
2	14.235544932919161	22.527752562896094	40.33078223502861	22.905920269156137
3	15.13495282272504	27.886807851497764	33.23603627611823	23.742203049658976
4	12.341125425761373	18.453351543567152	40.16347571182305	29.042047318848425
5	13.027590169804878	38.07526711581542	35.84257030788064	13.054572406499055
6	28.379650063708056	40.33927664287677	18.159711523247196	13.121361770167972
7	25.417183687405792	32.75035601562305	24.029513903346963	17.802946393624197
8	24.59947201425728	35.85006537362902	21.70454450819877	17.845918103914922
9	25.198827438602923	15.57441351110519	21.007919786140793	38.2188392641511
10	14.622873275093898	28.785133120695544	34.47280540310962	22.119188201100943
11	32.61686056679353	23.816154365043012	25.435588237743485	18.131396830419973
12	21.609273894685998	25.883460055463487	31.5084236211161	20.998842428734417
13	27.97541618434531	22.02899757659541	26.983652428817695	23.01193381024159
14	21.4384696741312	21.569883160252836	28.49199277142548	28.499654394190493
15	23.05623797666536	29.219680377085083	24.515777113400343	23.20830453284921
16	22.75451994103882	28.524887782210044	25.386953588887316	23.33363868786382
17	21.79998167872817	28.549038549621496	27.08891646332831	22.562063308322024
18	22.79982344956237	27.485238884401102	27.836174518442025	21.8787631475945
19	23.57106571507091	27.340167722915748	27.754561580292975	21.33420498172037
20	23.278091922817477	26.902788997243483	27.47041530992097	22.348703770018073
21	23.21405074992297	26.989232088874825	27.643134936167023	22.153582225035183
22	22.03541002173569	27.95817753312403	27.813939156721823	22.19247328841846
23	22.642926739896236	26.787198427701764	27.813106371638675	22.75676846076333
24	22.66507882310812	27.335254290925143	27.596498971510425	22.403167914456315
25	22.615444832152168	27.240483348462263	27.6923525345814	22.45171928480417
26	23.159919719517987	27.188600837781795	27.550112842378766	22.101366600321455
27	22.461546148785384	27.810774573405837	27.37564436745809	22.352034910350685
28	22.522339459855594	27.060018820942876	27.65254540760666	22.765096311594867
29	22.84312827388636	27.400627919952697	27.65945752379683	22.09678628236411
30	22.285995053256606	27.178024467225743	27.748981920235842	22.786998559281805
31	23.62403084635948	26.715412353533925	27.120229182454885	22.540327617651712
32	22.22828304699406	27.34058411545732	28.068271721116933	22.36286111643168
33	22.12959801464036	26.91894502785666	28.104414593725796	22.84704236377718
34	22.63851297895552	27.12822391925316	27.5871717785791	22.646091323212218
35	22.276501303308656	27.15853729627995	28.533715304091473	22.031246096319922
36	22.923825148443942	27.11148493908177	27.072010926140287	22.892678986333998
37	22.822808317857408	26.965914106546524	27.712089541052144	22.499188034543927
38	22.60978189358672	26.794360379416883	27.66853488120321	22.927322845793185
39	22.63060152066556	26.881469699114753	27.737489486088325	22.750439294131365
40	22.241357772799574	27.01263334971144	28.380316291774584	22.365692585714406
41	22.634182496523124	26.516210161643585	27.596498971510425	23.25310837032287
42	22.481199876747805	26.847158953688822	27.996902039490667	22.6747391300727
43	22.77633891021744	26.33432989948284	28.14488794876706	22.744443241532657
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	16.5
2	26.0
3	108.5
4	191.0
5	191.0
6	244.0
7	297.0
8	324.5
9	352.0
10	536.0
11	720.0
12	720.0
13	1111.5
14	1503.0
15	3689.5
16	5876.0
17	6791.5
18	7707.0
19	7707.0
20	8216.5
21	8726.0
22	11029.0
23	13332.0
24	17469.0
25	21606.0
26	21606.0
27	26798.5
28	31991.0
29	37712.5
30	43434.0
31	49818.5
32	56203.0
33	56203.0
34	63204.0
35	70205.0
36	74463.0
37	78721.0
38	82163.5
39	85606.0
40	85606.0
41	87595.5
42	89585.0
43	90590.5
44	91596.0
45	91525.5
46	91455.0
47	91455.0
48	90459.0
49	89463.0
50	86211.0
51	82959.0
52	83084.0
53	83209.0
54	83209.0
55	74586.0
56	65963.0
57	61116.5
58	56270.0
59	49036.5
60	41803.0
61	41803.0
62	36011.0
63	30219.0
64	24994.0
65	19769.0
66	16381.0
67	12993.0
68	12993.0
69	10679.5
70	8366.0
71	6753.0
72	5140.0
73	3899.0
74	2658.0
75	2658.0
76	2040.5
77	1423.0
78	1066.5
79	710.0
80	542.5
81	375.0
82	375.0
83	287.0
84	199.0
85	141.0
86	83.0
87	56.5
88	30.0
89	30.0
90	22.0
91	14.0
92	9.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1200790.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.5354357606759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98602841847207	44.29424692540569
2	13.536301553891409	15.57634016982113
3	4.126001204613687	7.121738317695664
4	1.8239534082457713	4.197678166023618
5	0.9386998728721315	2.7004253117094574
6	0.5574222183813226	1.924291814235122
7	0.3720612298902502	1.498469349397199
8	0.2676264576356464	1.2318403888922385
9	0.20823606391115373	1.0782857410394566
>10	1.0593507053564768	11.082771841375173
>50	0.08538568411158089	3.3226323472675117
>100	0.036027728354119645	3.4825872092900454
>500	0.0017432725586445106	0.7101236198835816
>1k	0.001162181705763007	1.7785687979640692
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	4111	0.34235794768444106	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3615	0.301051807560023	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	3470	0.2889764238542959	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3356	0.279482673906345	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	1983	0.165141281989357	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	1982	0.16505800348104166	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1639	0.1364934751288735	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.327850831535905E-5	0.0
3	0.0	0.0	0.0	8.327850831535905E-5	0.0
4	0.0	0.0	0.0	1.665570166307181E-4	0.0
5	0.0	0.0	0.0	1.665570166307181E-4	0.0
6	0.0	0.0	0.0	1.665570166307181E-4	0.0
7	0.0	0.0	0.0	1.665570166307181E-4	0.0
8	0.0	0.0	0.0	1.665570166307181E-4	0.0
9	0.0	0.0	0.0	2.498355249460772E-4	0.0
10	0.0	0.0	0.0	2.498355249460772E-4	0.0
11	0.0	0.0	0.0	2.498355249460772E-4	0.0
12	0.0	0.0	0.0	2.498355249460772E-4	0.0
13	0.0	0.0	0.0	2.498355249460772E-4	0.0
14	0.0	0.0	0.0	2.498355249460772E-4	0.0
15	0.0	0.0	0.0	2.498355249460772E-4	0.0
16	0.0	0.0	0.0	2.498355249460772E-4	0.0
17	0.0	0.0	0.0	3.331140332614362E-4	0.0
18	0.0	0.0	0.0	4.996710498921544E-4	0.0
19	0.0	0.0	0.0	5.829495582075133E-4	0.0
20	0.0	0.0	0.0	8.327850831535905E-4	0.0
21	0.0	0.0	0.0	9.160635914689496E-4	0.0
22	0.0	0.0	0.0	9.993420997843087E-4	0.0
23	0.0	0.0	0.0	0.0013324561330457449	0.0
24	0.0	0.0	0.0	0.0017488486746225402	0.0
25	0.0	0.0	0.0	0.0018321271829378992	0.0
26	0.0	0.0	0.0	0.0024150767411454128	0.0
27	0.0	0.0	0.0	0.002998026299352926	0.0
28	0.0	0.0	0.0	0.007578344256697674	0.0
29	0.0	0.0	0.0	0.017821600779486836	0.0
30	0.0	0.0	0.0	0.03264517525962075	0.0
31	0.0	0.0	0.0	0.07353492284246205	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAACGC	50	9.091531E-6	25.899998	3
GGTATCA	685	0.0	24.576643	1
GTATCAA	1780	0.0	23.592697	1
CTTATAC	820	0.0	21.432926	1
GATTACG	45	0.0038259053	20.555557	1
TATACAC	970	0.0	20.216496	3
CGAACTA	85	1.2454038E-6	19.588234	24
TTATACA	1065	0.0	18.413145	2
GGTTCGC	115	6.405571E-8	17.695652	23
TTCGGAC	65	0.0015799896	17.076923	3
GCCGTCT	380	0.0	17.039474	36
CGGTTCG	120	1.0412805E-7	16.958332	22
CCGTCTT	385	0.0	16.818182	37
GACGTTA	125	1.6577724E-7	16.279999	20
TGCGTTA	70	0.0025925848	15.857142	37
CGTTACC	145	5.3452823E-8	15.310344	22
TTCGCAC	85	5.3643994E-4	15.235293	27
ACCCGCA	160	1.0963049E-8	15.03125	26
CGTCTTC	235	0.0	14.957446	37
ACAATCG	75	0.0041045467	14.8	37
>>END_MODULE