FastQCFastQC Report
Fri 10 Feb 2017
ERR1631075.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631075.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111824
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT224652.020553612802026No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG168081.5117500611607593No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT123571.1114169149073954TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC92110.8284584610513894No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC42020.3779375152901898RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC40290.3623774985969002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39240.3529335578292967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35630.3204643900473456No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG22710.2042589474593101No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20140.18114377815193772No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT18740.16855185712846638No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA15460.13905078501633353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13730.12349076832304394No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT12940.1163853271740851No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT11280.10145490653196909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG800.034.68751
CTTATAC26700.033.119851
TTATACA28900.030.854672
TATACAC30150.029.8208943
CGTCTTC6050.029.04958537
GGTATCA8750.028.3314291
GCCGTCT18250.025.54520436
CCGTCTT18500.025.237
TTACGGG1200.024.6666663
GTATCAA21050.022.1472681
TGCCGTC23300.020.08798235
CGGATCG656.9018686E-519.92307937
ACAATCG656.9018686E-519.92307937
TACACAT47750.019.1392675
ATACACA48200.018.9989624
ATGCCGT24850.018.8350134
CGCGAGG500.007034741318.523
GACGGAT701.21915175E-418.535
ACGGTAC2650.017.4528313
CGGTGCT852.7229858E-517.41176434
Produced by FastQC (version 0.11.5)