##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631075.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1111824
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.533760739109788	31.0	31.0	34.0	30.0	34.0
2	31.526815395242412	31.0	31.0	34.0	30.0	34.0
3	31.000772604297083	31.0	31.0	34.0	27.0	34.0
4	35.14540610744147	37.0	35.0	37.0	32.0	37.0
5	35.335364230309835	37.0	35.0	37.0	33.0	37.0
6	35.52795496409504	37.0	35.0	37.0	33.0	37.0
7	35.53513685619307	37.0	35.0	37.0	33.0	37.0
8	35.54931715811136	37.0	35.0	37.0	33.0	37.0
9	37.33512498381039	39.0	37.0	39.0	34.0	39.0
10	37.17276835182547	39.0	37.0	39.0	33.0	39.0
11	37.228482205816746	39.0	37.0	39.0	34.0	39.0
12	37.16977147462188	39.0	37.0	39.0	33.0	39.0
13	37.23052929240599	39.0	37.0	39.0	34.0	39.0
14	38.37360409561226	40.0	38.0	41.0	34.0	41.0
15	38.38097126883392	40.0	38.0	41.0	34.0	41.0
16	38.38320633481558	40.0	38.0	41.0	34.0	41.0
17	38.354994135762496	40.0	38.0	41.0	34.0	41.0
18	38.3997871965347	40.0	38.0	41.0	34.0	41.0
19	38.4221090748176	40.0	38.0	41.0	34.0	41.0
20	38.42359851919009	40.0	38.0	41.0	34.0	41.0
21	38.389603030695504	40.0	38.0	41.0	34.0	41.0
22	38.3157649052368	40.0	38.0	41.0	34.0	41.0
23	38.313636870583835	40.0	38.0	41.0	34.0	41.0
24	38.25464012289715	40.0	38.0	41.0	34.0	41.0
25	38.247302630632184	40.0	38.0	41.0	34.0	41.0
26	38.127844874728375	40.0	38.0	41.0	33.0	41.0
27	38.05276014908834	40.0	37.0	41.0	33.0	41.0
28	38.053977967735904	40.0	37.0	41.0	33.0	41.0
29	37.974233331894254	40.0	37.0	41.0	33.0	41.0
30	37.91845022233735	40.0	37.0	41.0	33.0	41.0
31	37.891524198074514	40.0	37.0	41.0	33.0	41.0
32	37.79992606743513	40.0	37.0	41.0	33.0	41.0
33	37.76504554677719	40.0	37.0	41.0	33.0	41.0
34	37.680909928190076	40.0	37.0	41.0	33.0	41.0
35	37.65419796658464	40.0	37.0	41.0	33.0	41.0
36	37.63529389543669	40.0	37.0	41.0	33.0	41.0
37	37.55042344831556	40.0	37.0	41.0	32.0	41.0
38	37.51588830606283	40.0	37.0	41.0	32.0	41.0
39	37.4572261437062	40.0	37.0	41.0	32.0	41.0
40	37.363258033645614	39.0	37.0	41.0	32.0	41.0
41	37.31726784095324	39.0	36.0	41.0	31.0	41.0
42	37.252875455108004	39.0	36.0	41.0	31.0	41.0
43	36.5881614356229	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	9.0
11	20.0
12	25.0
13	11.0
14	9.0
15	10.0
16	13.0
17	19.0
18	25.0
19	47.0
20	102.0
21	188.0
22	391.0
23	792.0
24	1383.0
25	2386.0
26	3693.0
27	5981.0
28	9028.0
29	12611.0
30	17603.0
31	23388.0
32	30766.0
33	40136.0
34	53507.0
35	71898.0
36	100060.0
37	148857.0
38	263706.0
39	325152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.36533480119155	22.074087265610384	15.096004403574668	24.464573529623394
2	13.533706773733972	22.196768553296206	41.553879031213576	22.71564564175625
3	14.528018823123084	28.82893335635856	34.40085840924463	22.242189411273728
4	11.430945905107283	19.59689663112147	38.06708615752134	30.90507130624991
5	12.244473945516557	38.40607865993179	35.95865892443408	13.390788470117574
6	26.98286779202464	40.4320288103153	17.956349206349206	14.628754191310856
7	24.226406337693735	33.18609779965174	22.771499805724645	19.815996056929876
8	24.409348961706172	35.183086531681276	22.71249766150038	17.695066845112176
9	24.117216393961634	15.049324353494796	19.532138180143622	41.30132107239995
10	14.266196808127903	29.12529321187526	33.631851803882626	22.976658176114206
11	30.950672048813484	23.430866755889422	25.383783764336805	20.234677430960296
12	20.45719466390364	26.511120465109585	29.353926520744288	23.677758350242485
13	28.874534098922133	21.0893090992819	27.17651354890702	22.859643252888947
14	21.690483385859633	22.445279108923717	26.906236958367515	28.958000546849146
15	23.722279785289757	28.562524284419116	23.181186950452588	24.534008979838536
16	24.310862150844017	27.676772582710935	23.631078300162617	24.381286966282435
17	20.814535394091152	30.840942451323233	25.04820906906129	23.29631308552433
18	23.86034120508282	26.331055994473946	26.103681877707263	23.704920922735973
19	23.819867173221663	27.92249492725467	26.524072155305156	21.73356574421851
20	22.753961058584814	25.572032983637698	27.268704399257437	24.405301558520055
21	23.849728014505896	26.007893335635856	25.889529278015228	24.252849371843023
22	20.807519895235217	30.169343349307088	27.435097641353305	21.58803911410439
23	22.404535250183482	26.097385917195524	26.518046021672493	24.980032810948497
24	23.02063995740333	29.259487113068257	26.543409748305486	21.17646318122293
25	22.753961058584814	27.73919253407014	26.828886586366185	22.67795982097886
26	24.316078803839456	28.1202780296162	25.9445739613464	21.619069205197945
27	21.455734001064915	29.011786038077968	28.215436975636432	21.317042985220684
28	20.97787059822418	26.199380477485647	27.23668494294061	25.586063981349565
29	21.47048453712098	28.960968642518957	27.574418253248716	21.99412856711134
30	21.719174977334543	26.01787693016161	29.673041776396264	22.589906316107587
31	22.88824490207083	26.898501921167377	25.9888255695146	24.22442760724719
32	22.973869965030435	26.275651541970674	29.12997021111255	21.62050828188634
33	22.451485127142426	25.674207428513867	28.56657168760523	23.307735756738477
34	21.40950366245017	29.719182172717986	26.570842147678047	22.300472017153794
35	23.455600886471238	26.080476766106862	29.947815481587014	20.516106865834878
36	24.821194721466707	25.789513448171654	26.030378908892054	23.358912921469585
37	22.289768884283845	27.26807480320626	27.90630531451021	22.535850997999685
38	22.176351652779577	25.107570982457656	28.847011757256546	23.869065607506222
39	23.802418368374852	25.7588431262502	26.869810329692466	23.568928175682483
40	20.81750348976097	25.699840982025933	31.55931154571227	21.92334398250083
41	22.37602354329462	25.939537192937014	26.72914058340169	24.955298680366678
42	23.16247895350343	25.186810142612497	29.234932867072487	22.41577803681158
43	22.868097828433275	24.580509145332353	29.79788167911468	22.753511347119687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	23.5
2	29.0
3	117.5
4	206.0
5	206.0
6	279.5
7	353.0
8	353.5
9	354.0
10	566.0
11	778.0
12	778.0
13	1223.5
14	1669.0
15	3827.5
16	5986.0
17	6925.0
18	7864.0
19	7864.0
20	8077.0
21	8290.0
22	10132.0
23	11974.0
24	15447.0
25	18920.0
26	18920.0
27	23611.5
28	28303.0
29	33826.5
30	39350.0
31	45287.0
32	51224.0
33	51224.0
34	55339.5
35	59455.0
36	62679.0
37	65903.0
38	69430.5
39	72958.0
40	72958.0
41	73723.0
42	74488.0
43	74572.5
44	74657.0
45	75774.5
46	76892.0
47	76892.0
48	78213.5
49	79535.0
50	96543.0
51	113551.0
52	94055.5
53	74560.0
54	74560.0
55	79917.5
56	85275.0
57	72252.0
58	59229.0
59	46123.0
60	33017.0
61	33017.0
62	28976.0
63	24935.0
64	20482.5
65	16030.0
66	13345.5
67	10661.0
68	10661.0
69	8488.5
70	6316.0
71	5377.0
72	4438.0
73	3403.5
74	2369.0
75	2369.0
76	1775.0
77	1181.0
78	906.5
79	632.0
80	426.0
81	220.0
82	220.0
83	167.0
84	114.0
85	88.5
86	63.0
87	42.0
88	21.0
89	21.0
90	13.5
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1111824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.28943796052128
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0600495324538	38.968254065856215
2	12.49742571350675	12.319821787442287
3	3.5830284114743995	5.298163697944576
4	1.4346738029273833	2.828570616118976
5	0.764370730585541	1.8837701852017175
6	0.4655905224411962	1.3769217102523217
7	0.32977059854140156	1.1377945220607266
8	0.2221712038277712	0.8760555014146606
9	0.1772915755779576	0.7864741903835528
>10	1.2168530333581573	11.885108850464421
>50	0.1516829076273954	5.175822200844472
>100	0.08960931635319884	7.880601755405987
>500	0.00419758488960144	1.3723541433045585
>1k	0.0025550516719313114	2.7373319730438985
>5k	1.8250369085223654E-4	0.8285759054783881
>10k+	5.475110725567096E-4	4.644378894783322
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	22465	2.020553612802026	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	16808	1.5117500611607593	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	12357	1.1114169149073954	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	9211	0.8284584610513894	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	4202	0.3779375152901898	RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	4029	0.3623774985969002	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3924	0.3529335578292967	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3563	0.3204643900473456	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	2271	0.2042589474593101	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2014	0.18114377815193772	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	1874	0.16855185712846638	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	1546	0.13905078501633353	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1373	0.12349076832304394	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	1294	0.1163853271740851	No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT	1128	0.10145490653196909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.6982687907438585E-4	0.0
6	0.0	0.0	0.0	2.6982687907438585E-4	0.0
7	0.0	0.0	0.0	2.6982687907438585E-4	0.0
8	0.0	0.0	0.0	3.5976917209918117E-4	0.0
9	0.0	0.0	0.0	6.295960511735671E-4	0.0
10	0.0	0.0	0.0	6.295960511735671E-4	0.0
11	0.0	8.994229302479529E-5	0.0	6.295960511735671E-4	0.0
12	0.0	8.994229302479529E-5	0.0	6.295960511735671E-4	0.0
13	0.0	8.994229302479529E-5	0.0	6.295960511735671E-4	0.0
14	0.0	8.994229302479529E-5	0.0	6.295960511735671E-4	0.0
15	0.0	8.994229302479529E-5	0.0	7.195383441983623E-4	0.0
16	0.0	8.994229302479529E-5	0.0	8.994229302479529E-4	0.0
17	0.0	8.994229302479529E-5	0.0	9.893652232727482E-4	0.0
18	0.0	8.994229302479529E-5	0.0	0.0010793075162975434	0.0
19	0.0	8.994229302479529E-5	0.0	0.0011692498093223388	0.0
20	0.0	8.994229302479529E-5	0.0	0.00152901898142152	0.0
21	0.0	8.994229302479529E-5	0.0	0.002068672739570292	0.0
22	0.0	8.994229302479529E-5	0.0	0.0023384996186446775	0.0
23	0.0	8.994229302479529E-5	0.0	0.0032379225488926304	0.0
24	0.0	8.994229302479529E-5	0.0	0.003687634014016607	0.0
25	0.0	8.994229302479529E-5	0.0	0.0038675186000661976	0.0
26	0.0	8.994229302479529E-5	0.0	0.004047403186115788	0.0
27	0.0	8.994229302479529E-5	0.0	0.005126710702413332	0.0
28	0.0	8.994229302479529E-5	0.0	0.010433305990876253	0.0
29	0.0	8.994229302479529E-5	0.0	0.024374361409719523	0.0
30	0.0	8.994229302479529E-5	0.0	0.0438918389961001	0.0
31	0.0	8.994229302479529E-5	0.0	0.09398969621091108	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	80	0.0	34.6875	1
CTTATAC	2670	0.0	33.11985	1
TTATACA	2890	0.0	30.85467	2
TATACAC	3015	0.0	29.820894	3
CGTCTTC	605	0.0	29.049585	37
GGTATCA	875	0.0	28.331429	1
GCCGTCT	1825	0.0	25.545204	36
CCGTCTT	1850	0.0	25.2	37
TTACGGG	120	0.0	24.666666	3
GTATCAA	2105	0.0	22.147268	1
TGCCGTC	2330	0.0	20.087982	35
CGGATCG	65	6.9018686E-5	19.923079	37
ACAATCG	65	6.9018686E-5	19.923079	37
TACACAT	4775	0.0	19.139267	5
ATACACA	4820	0.0	18.998962	4
ATGCCGT	2485	0.0	18.83501	34
CGCGAGG	50	0.0070347413	18.5	23
GACGGAT	70	1.21915175E-4	18.5	35
ACGGTAC	265	0.0	17.452831	3
CGGTGCT	85	2.7229858E-5	17.411764	34
>>END_MODULE