FastQCFastQC Report
Fri 10 Feb 2017
ERR1631074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293790
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT42510.3285695514728047No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT40370.3120290000695631No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC35270.27260992896837977No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG31810.24586679445659654No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC31010.2396834107544501No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG30260.23388648853368785No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC29920.2312585504602756No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT29200.22569350512834388No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT26130.20196477017135703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25870.19995517046815944No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC25510.19717264780219354No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA25290.1954722172841033No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT23510.18171418854682753No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA22550.17429412810425185No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA21860.16896095966115057No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA21520.16633302158773836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21370.1651736371435859No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC20930.16177277610740537No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG20100.15535751551642848No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT19240.1487103780366211RNA PCR Primer, Index 29 (95% over 23bp)
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT18320.14159948677915274No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA17810.13765757966903439No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG17420.13464318011423804No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC17220.13309733418870143No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG17060.13186065744827213No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG16510.12760958115304646No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC15880.12274016648760619No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA15860.1225855818950525No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA15580.12042139759930127No Hit
TTGTAATGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGA14610.11292404486044877No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG14180.10960047612054508No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC14140.10929130693543776No Hit
GCATTTACTTTGTGGCTGGATTATTTGTAATGCTGGTACAAGG14040.10851838397266944No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT13460.10403543078861328No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA13390.10349438471467548No Hit
ATGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAA13260.1024895848630767No Hit
TGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAAA13070.10102103123381694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5400.026.0370371
CTTATAC9000.025.6944451
GATTACG809.786163E-1025.43751
GTATCAA15600.024.5480791
TATACAC10650.024.4929583
GCGGTAT400.001931318123.1256
CAACCGT400.001931318123.1256
TTACGGG1005.347829E-1022.23
TTATACA11050.021.9321272
CGGACGT603.7267513E-521.58333436
CCGTCTT3550.021.36619837
GCCGTCT3650.020.7808236
GTATACG656.902986E-519.9230771
ACTGCGT500.007035346718.58
CGTCTTC1601.8189894E-1218.537
GTACTAA902.152643E-618.51
GGACGTC801.616927E-518.537
CTCGCCG500.007035346718.530
TGCCGTC4150.018.2771135
TAACGCT1156.4066626E-817.6956524
Produced by FastQC (version 0.11.5)