FastQCFastQC Report
Fri 10 Feb 2017
ERR1631073.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631073.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462618
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT278126.011871565741065No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG222234.803747368239023No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT168453.6412331556489375TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC118412.559563181717962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67211.4528185241387062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66991.4480629806881704No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC65351.4126125658750848RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC58091.2556796320074013No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA46441.003851990194934No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA28650.6193014538993294No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG26960.5827702337565767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25020.5408349869654877No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT24230.5237582627567453No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA22480.4859300762183919No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA21860.47252809013051805No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT20680.4470210843503712No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT19820.4284312326800946No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC19450.420433273240557No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG18630.40270806583401425No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC17800.384766697361538No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT17540.37914650964726837No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG15630.3378597460539797No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG13650.295059854999157No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT13300.28749421769148625No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT12680.2740922316036125No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG12450.2691205270871432No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG11930.25788015165860384No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG11450.24750442049379834No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11410.24663977623006453No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG11350.24534280983446385No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG11240.24296503810919595No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG10810.23367011227405765No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10730.23194082374659006No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG10240.22134893151585108No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10020.21659338806531525No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9600.2075146232961104No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG9270.2003813081203066No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC8710.1882762884280335No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT8670.1874116441642997No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8490.18352074497749762No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8360.1807106511203628No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG8200.17725207406542762No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA8020.17336117487862557No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7790.16838947036215626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.16817330929622282No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT7720.16687634290062212No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA7060.15260971254901454No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG6870.14850265229627901No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6700.14482791417541038No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG6550.14158549818640867No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC6540.1413693371204752No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6420.13877540432927382No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6420.13877540432927382No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6270.13553298834027208No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6210.1342360219446714No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.13250673341720381No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA6110.13207441128533692No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG6110.13207441128533692No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG6030.13034512275786936No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT5850.12645422357106728No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5830.1260219014392004No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG5760.12450877397766624No Hit
ATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT5660.12234716331833174TruSeq Adapter, Index 8 (95% over 21bp)
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG5610.12126635798866452No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5610.12126635798866452No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA5590.12083403585679761No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC5540.11975323052713037No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5510.11910474732933003No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5480.11845626413152968No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5430.11737545880186244No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5370.11607849240626174No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5370.11607849240626174No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5350.11564617027439486No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC5290.11434920387879417No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA5280.11413304281286071No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA5220.11283607641726004No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA5160.11153911002165934No Hit
TCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT5150.11132294895572589TruSeq Adapter, Index 8 (95% over 24bp)
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5090.1100259825601252No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5090.1100259825601252No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG4930.10656740550519003No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC4910.10613508337332313No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4690.10137953992278725No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA4670.10094721779092036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG308.301244E-637.01
TCGCCGA200.001840799737.01
ACATTTC200.001840799737.036
TAGTTCC403.824789E-837.04
GTTCCTT403.824789E-837.06
AGTTCCT403.824789E-837.05
GTATACA355.621605E-737.02
TTACGGG800.037.03
TACAGCC200.001840799737.03
GATTACG800.037.01
TTCTGCT403.824789E-837.037
CTTATAC30200.035.284771
TCAGAGT900.034.9444473
ACTCAGA900.034.9444471
ATTACGG850.034.823532
CAGACAC603.45608E-1133.91666818
TTTACAC507.3123374E-933.3000032
ATTGTTA507.3123374E-933.30000314
TGTTACG507.3123374E-933.30000316
TTATACA32200.032.978262
Produced by FastQC (version 0.11.5)