FastQCFastQC Report
Fri 10 Feb 2017
ERR1631072.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631072.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380327
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT78530.5689231609611346No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG54890.39765939520128196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46830.33926743445574853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42220.3058695512005489No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT39700.287613007642392TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC29720.2153112994239771No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21330.1545286008315421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15120.10953926134894122No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC14190.10280172741676429RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC14060.10185992159828794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.027.4730531
CTTATAC11950.026.9372391
GATTACG1700.025.0294111
GTATCAA26600.024.9680461
TATACAC14150.024.7102493
GGACCGT1150.024.1304326
GCCGTCT5950.023.94117536
CGTCTTC2500.023.68000237
CCGTCTT6100.023.65573937
TTATACA14450.023.4290662
TTACGGG1850.022.03
ATTACGG1950.021.8205132
CGCCGTT855.1826646E-821.76470625
TGGACCG759.267984E-619.7333345
AGGACCG1254.129106E-1019.2400025
TGCCGTC8000.018.03749835
TACACAT19600.018.0280635
GACCGTT1054.798985E-717.6190477
CGGCGTA953.607449E-617.52631612
CTGACGC953.607449E-617.52631627
Produced by FastQC (version 0.11.5)