##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631072.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1380327
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.564882813999873	31.0	31.0	34.0	30.0	34.0
2	31.534529136936392	31.0	31.0	34.0	30.0	34.0
3	31.015163798143483	31.0	31.0	34.0	27.0	34.0
4	35.16258466290959	37.0	35.0	37.0	32.0	37.0
5	35.36638492183374	37.0	35.0	37.0	33.0	37.0
6	35.55911026879863	37.0	35.0	37.0	33.0	37.0
7	35.569588220762185	37.0	35.0	37.0	33.0	37.0
8	35.58775493053458	37.0	35.0	37.0	33.0	37.0
9	37.359128670235386	39.0	37.0	39.0	34.0	39.0
10	37.19977874807926	39.0	37.0	39.0	33.0	39.0
11	37.262284226853495	39.0	37.0	39.0	34.0	39.0
12	37.21260252099684	39.0	37.0	39.0	34.0	39.0
13	37.268374812634974	39.0	37.0	39.0	34.0	39.0
14	38.44172286711772	40.0	38.0	41.0	34.0	41.0
15	38.446707917761515	40.0	38.0	41.0	34.0	41.0
16	38.451408977727745	40.0	38.0	41.0	34.0	41.0
17	38.44815684978994	40.0	38.0	41.0	34.0	41.0
18	38.471672292145264	40.0	38.0	41.0	34.0	41.0
19	38.50881204236387	40.0	38.0	41.0	34.0	41.0
20	38.49334759082449	40.0	38.0	41.0	34.0	41.0
21	38.44902331114294	40.0	38.0	41.0	34.0	41.0
22	38.41007384482083	40.0	38.0	41.0	34.0	41.0
23	38.37962019144739	40.0	38.0	41.0	34.0	41.0
24	38.360119015276815	40.0	38.0	41.0	34.0	41.0
25	38.33488513953578	40.0	38.0	41.0	34.0	41.0
26	38.23184361386831	40.0	38.0	41.0	34.0	41.0
27	38.18842853903459	40.0	38.0	41.0	34.0	41.0
28	38.146208108658314	40.0	38.0	41.0	33.0	41.0
29	38.08822474674479	40.0	38.0	41.0	33.0	41.0
30	38.00139604600939	40.0	37.0	41.0	33.0	41.0
31	37.957150733123385	40.0	37.0	41.0	33.0	41.0
32	37.902778834290714	40.0	37.0	41.0	33.0	41.0
33	37.8840912334541	40.0	37.0	41.0	33.0	41.0
34	37.82531820358509	40.0	37.0	41.0	33.0	41.0
35	37.78255877049424	40.0	37.0	41.0	33.0	41.0
36	37.73370730268987	40.0	37.0	41.0	33.0	41.0
37	37.69400873850906	40.0	37.0	41.0	33.0	41.0
38	37.64235648509375	40.0	37.0	41.0	33.0	41.0
39	37.57915407001384	40.0	37.0	41.0	32.0	41.0
40	37.525980437968684	40.0	37.0	41.0	32.0	41.0
41	37.462591110657115	40.0	37.0	41.0	32.0	41.0
42	37.39736671093154	40.0	37.0	41.0	32.0	41.0
43	36.80827079380466	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	1.0
9	2.0
10	8.0
11	9.0
12	4.0
13	5.0
14	2.0
15	7.0
16	6.0
17	7.0
18	19.0
19	49.0
20	109.0
21	226.0
22	464.0
23	877.0
24	1490.0
25	2573.0
26	4362.0
27	6709.0
28	10286.0
29	14806.0
30	20667.0
31	27507.0
32	36984.0
33	48135.0
34	63680.0
35	87124.0
36	122107.0
37	184800.0
38	328107.0
39	419194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.14099557568605	21.648710776504405	14.872925038777044	23.337368609032495
2	14.044135918517858	23.04772709655031	40.774758444919215	22.13337854001262
3	15.065922784963274	28.242075971853048	33.82553554338936	22.866465699794325
4	12.11473802946693	18.725780195562354	40.23271297308536	28.92676880188535
5	12.528770356589417	38.65823098439717	35.9369917418119	12.876006917201504
6	27.565352267977083	40.95493314265388	18.14461355896103	13.335101030408012
7	24.996105995173608	33.20981187791009	23.823340411366292	17.970741715550012
8	24.38827900924926	35.771233917760064	21.9363237841468	17.904163288843876
9	25.227210653707417	15.585292470552266	20.828760141618616	38.3587367341217
10	14.555464031349095	29.172942353514784	34.285426569211495	21.986167045924628
11	31.78406276193974	24.469346756239645	25.334431623810875	18.412158858009732
12	21.319803206051898	26.578122430409607	31.134941213205273	20.96713315033322
13	27.975110245615713	22.48235381905882	27.212319979251294	22.330215956074177
14	21.550835417984292	22.07006021037044	28.623362435133124	27.755741936512145
15	23.188708182916077	29.39238310921977	24.68045615278119	22.738452555082965
16	22.569869313575698	28.960311578343394	25.1617189260226	23.308100182058308
17	21.56409314604438	28.87598373428905	26.75583394369595	22.80408917597062
18	22.84654288440348	27.45646502604093	27.809062635158188	21.8879294543974
19	24.136237282904702	26.984982543991386	27.82688449910782	21.05189567399609
20	24.032493749669463	26.60717351757953	27.616209782174806	21.7441229505762
21	22.985640359132294	27.423719162198523	27.27889840595743	22.311742072711755
22	21.757308232034873	28.587646260632443	27.815727722488948	21.839317784843736
23	22.117440287699942	27.246514775122126	27.899620886934763	22.73642405024317
24	22.576606847507875	27.876872654088487	27.405390172038945	22.141130326364696
25	22.8108267099028	27.670399840037902	27.55397815155394	21.964795298505354
26	22.62717457529991	27.56940927765667	27.576653937798795	22.226762209244622
27	22.542049818629934	28.032415507339927	27.447626540667535	21.977908133362604
28	21.47607052531755	27.905561508251303	28.085011739971762	22.533356226459382
29	22.457287294967063	27.52840450125224	27.566294073795554	22.448014129985143
30	22.721282710546124	27.54912422925872	27.764000849074165	21.96559221112099
31	22.73214970075931	27.879770518145335	27.610631393865365	21.777448387229985
32	22.058758540548727	27.328451881329567	28.38110099998044	22.231688578141267
33	21.693917455791272	26.951946893743294	28.95618212206238	22.397953528403054
34	21.858081454611842	27.854269314445055	27.691699140855754	22.59595009008735
35	22.381435703279006	27.55738314182074	28.474194882806753	21.586986272093498
36	22.57414366305955	27.28346254184697	27.744222926886163	22.398170868207316
37	22.770691292715423	27.1987000181841	27.648810752814367	22.381797936286112
38	22.5596543427753	26.84298720520572	28.079578244865168	22.517780207153812
39	22.680929953554486	26.935284175416406	27.85499378045927	22.528792090569844
40	21.76976904747933	27.019612019470753	29.00095412174072	22.209664811309203
41	22.290442771893908	26.593408663309493	27.903243217005823	23.212905347790777
42	22.46351770268929	26.71424959448015	28.267722068756175	22.55451063407439
43	22.32579671338748	26.250808685188364	28.658933716430962	22.76446088499319
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	24.5
2	34.0
3	153.5
4	273.0
5	273.0
6	336.0
7	399.0
8	434.0
9	469.0
10	696.5
11	924.0
12	924.0
13	1434.5
14	1945.0
15	4732.0
16	7519.0
17	8745.5
18	9972.0
19	9972.0
20	10662.0
21	11352.0
22	14143.5
23	16935.0
24	22140.5
25	27346.0
26	27346.0
27	33467.5
28	39589.0
29	48146.5
30	56704.0
31	62944.5
32	69185.0
33	69185.0
34	74768.5
35	80352.0
36	84912.5
37	89473.0
38	93467.5
39	97462.0
40	97462.0
41	99669.5
42	101877.0
43	100262.0
44	98647.0
45	98347.0
46	98047.0
47	98047.0
48	97627.0
49	97207.0
50	101662.5
51	106118.0
52	98863.0
53	91608.0
54	91608.0
55	87953.0
56	84298.0
57	74266.5
58	64235.0
59	53496.0
60	42757.0
61	42757.0
62	37543.5
63	32330.0
64	26593.5
65	20857.0
66	17285.5
67	13714.0
68	13714.0
69	10945.0
70	8176.0
71	6682.5
72	5189.0
73	3992.0
74	2795.0
75	2795.0
76	2070.0
77	1345.0
78	995.0
79	645.0
80	466.0
81	287.0
82	287.0
83	216.0
84	145.0
85	97.5
86	50.0
87	46.5
88	43.0
89	43.0
90	25.5
91	8.0
92	4.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1380327.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.5055713520414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3259557483454	38.548860040608126
2	13.95418488168462	14.095281604029996
3	4.268757935138448	6.4678817543318345
4	1.7889112279169859	3.6139993465611733
5	0.9134890778585262	2.3068143900547144
6	0.5224435485628616	1.5831785951613184
7	0.3670522006980648	1.297672677859595
8	0.24429191940140457	0.9870482372843827
9	0.17958602744318344	0.8163085430575229
>10	1.1831782211511641	11.583095866303701
>50	0.14878108739536666	5.294040247332111
>100	0.0965542522040285	8.860847313474896
>500	0.004784208140401887	1.5814994993191012
>1k	0.0017397120510552315	1.9865578460126174
>5k	2.899520085092052E-4	0.976914038608984
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	7853	0.5689231609611346	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	5489	0.39765939520128196	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4683	0.33926743445574853	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4222	0.3058695512005489	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	3970	0.287613007642392	TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	2972	0.2153112994239771	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2133	0.1545286008315421	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1512	0.10953926134894122	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	1419	0.10280172741676429	RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	1406	0.10185992159828794	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.244660142125743E-5	0.0
5	0.0	0.0	0.0	7.244660142125743E-5	0.0
6	0.0	0.0	0.0	7.244660142125743E-5	0.0
7	0.0	0.0	0.0	7.244660142125743E-5	0.0
8	0.0	0.0	0.0	7.244660142125743E-5	0.0
9	0.0	0.0	0.0	1.4489320284251486E-4	0.0
10	0.0	0.0	0.0	1.4489320284251486E-4	0.0
11	0.0	0.0	0.0	2.1733980426377227E-4	0.0
12	0.0	0.0	0.0	2.1733980426377227E-4	0.0
13	0.0	0.0	0.0	2.1733980426377227E-4	0.0
14	0.0	0.0	0.0	3.6223300710628713E-4	0.0
15	0.0	0.0	0.0	3.6223300710628713E-4	0.0
16	0.0	0.0	0.0	3.6223300710628713E-4	0.0
17	0.0	0.0	0.0	3.6223300710628713E-4	0.0
18	0.0	0.0	0.0	3.6223300710628713E-4	0.0
19	0.0	0.0	0.0	3.6223300710628713E-4	0.0
20	0.0	0.0	0.0	3.6223300710628713E-4	0.0
21	0.0	0.0	0.0	5.071262099488019E-4	0.0
22	0.0	0.0	0.0	7.244660142125743E-4	0.0
23	0.0	0.0	0.0	0.001159145622740119	0.0
24	0.0	0.0	0.0	0.001521378629846406	0.0
25	0.0	0.0	0.0	0.0018111650355314356	0.0
26	0.0	0.0	0.0	0.0021733980426377228	0.0
27	0.0	0.0	0.0	0.0031876504625353267	0.0
28	0.0	0.0	0.0	0.009418058184763465	0.0
29	0.0	0.0	0.0	0.024052271671857466	0.0
30	0.0	0.0	0.0	0.04636582490960475	0.0
31	0.0	0.0	0.0	0.10352619343097687	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	835	0.0	27.473053	1
CTTATAC	1195	0.0	26.937239	1
GATTACG	170	0.0	25.029411	1
GTATCAA	2660	0.0	24.968046	1
TATACAC	1415	0.0	24.710249	3
GGACCGT	115	0.0	24.130432	6
GCCGTCT	595	0.0	23.941175	36
CGTCTTC	250	0.0	23.680002	37
CCGTCTT	610	0.0	23.655739	37
TTATACA	1445	0.0	23.429066	2
TTACGGG	185	0.0	22.0	3
ATTACGG	195	0.0	21.820513	2
CGCCGTT	85	5.1826646E-8	21.764706	25
TGGACCG	75	9.267984E-6	19.733334	5
AGGACCG	125	4.129106E-10	19.240002	5
TGCCGTC	800	0.0	18.037498	35
TACACAT	1960	0.0	18.028063	5
GACCGTT	105	4.798985E-7	17.619047	7
CGGCGTA	95	3.607449E-6	17.526316	12
CTGACGC	95	3.607449E-6	17.526316	27
>>END_MODULE