FastQCFastQC Report
Fri 10 Feb 2017
ERR1631071.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631071.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527774
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT286985.437554710917931No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG215734.087545047690868No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT156042.9565685312273815Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC115812.19431044348528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81171.5379689033563608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79781.5116318727334048No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA59831.133629167029827No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC59131.1203659141981226Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC51230.970680632240315No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA34410.6519836141985016No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT31650.5996885030334954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30000.5684251213587634No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG27640.523709011811874No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT26010.49282458021804787No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT24350.461371723502863No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG23540.44602424522617634No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA23520.4456452951452705No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC23200.43958209385077707No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA21350.4045292113669866No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG20700.39221333373754674No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC20180.38236063163399486No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT17990.34086559777480513No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG17250.326844444781289No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG16220.3073285156146381No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT15780.29899161383470957No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG14840.28118096003213494No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG14720.2789072595466999No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG14320.27132825792858306No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG13880.26299135614865454No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG13830.2620439809463899No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG13750.26052818062276656No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG13550.2567386798137081No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT12420.23532800024252806No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC12300.23305429975709302No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC11990.22718057350305243No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11900.22547529813897615No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11810.22377002277489985No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11330.21467522083315962No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG11260.2133488955499892No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA11110.21050676994319537No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT10460.19819089231375553No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9680.18341183915842765No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT9630.18246446395616306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.1820855138752572No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG8970.16995911128627025No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8840.16749593576038227No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA8810.16692751063902353No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8580.16256958470860633No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC8360.15840113381864207No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8270.15669585845456577No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG8090.1532853077264132No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG7870.14911685683644893No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA7840.14854843171509016No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7740.14665368131056095No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT7630.14456945586557882No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG7500.14210628033969086No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG7470.1415378552183321No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT7450.14115890513742624No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7030.13320095343840355No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG6940.13149567807432727No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG6900.1307377779125156No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6890.13054830287206268No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA6860.1299798777507039No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA6780.12846407742708052No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC6770.1282746023866276No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6710.1271377521439101No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6700.12694827710345716No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG6680.12656932702255133No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC6420.12164297597077538No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA6420.12164297597077538No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6400.12126402588986954No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6400.12126402588986954No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC6350.12031665068760493No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6220.11785347516171694No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA6190.11728505004035819No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA6190.11728505004035819No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG6170.11690609995945235No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6140.11633767483809358No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG6020.11406397435265853No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC5920.11216922394812932No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG5880.11141132378631763No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5690.10781129801771214No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5650.10705339785590044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC5560.10534812249182415No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5550.10515864745137124No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5480.10383232216820079No Hit
ATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT5400.10231652184457742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG355.623606E-737.01
TCGCCGA200.001841034437.01
GTTGACA251.232721E-437.02
TTCTACA308.303461E-637.02
CATGAGT200.001841034437.01
TTACTTT403.826608E-837.03
TGAGTAT200.001841034437.03
GTCAGAC200.001841034437.03
TCTACAC308.303461E-637.03
TACTCTC200.001841034437.035
CTTCTAC251.232721E-437.01
ACTCAGA700.037.01
GATTAGG200.001841034437.01
TATAACA200.001841034437.03
TAGTCAG200.001841034437.01
CTTATAC33000.035.4303051
ATTACGG1000.035.152
TTACGGG1000.035.153
GATTACG1000.035.151
TCAGAGT750.034.5333333
Produced by FastQC (version 0.11.5)