FastQCFastQC Report
Fri 10 Feb 2017
ERR1631070.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631070.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800379
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT240923.0100739774531817No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG191852.3969894262593097No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT163162.0385342444017147TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC103381.2916380864565413No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC61210.764762693673872RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC49970.6243292240301157No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44100.5509889689759476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41820.5225024644574633No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG24290.30348122576929176No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19110.23876188655624397No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA18430.2302659115244153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15430.19278366873693586No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT14560.18191381832856685No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT12450.15555130756803964No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10220.12768950709601326No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10150.12681492143097206No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8410.10507522061423401No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8370.10457545737706762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC29050.033.56111
CGTCTTC6900.033.51449237
TTATACA30050.032.444262
TATACAC32100.030.8333343
GATTACG601.2696546E-930.8333321
CGGATCG502.7190436E-729.59999837
GGTATCA6200.028.645161
TACGTTA405.9379112E-527.7519
CCGTCTT25300.026.54347837
GCCGTCT25750.026.0796136
GTATCAA19800.024.5732331
AATTACG551.9017227E-523.54545416
GTCAACC802.723209E-823.12515
TAGGACC1600.023.1254
TGGGACG400.001930677823.12511
TGCCGTC30450.022.05418835
ATGCCGT32150.020.88802534
GGGTCGA450.003824808620.55555524
AACCACG450.003824808620.55555535
GGGACGA450.003824808620.55555512
Produced by FastQC (version 0.11.5)