##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631070.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800379
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.482933710154814	31.0	31.0	34.0	28.0	34.0
2	31.49795409424785	31.0	31.0	34.0	30.0	34.0
3	30.99005596098848	31.0	31.0	34.0	27.0	34.0
4	35.10775395156545	37.0	35.0	37.0	32.0	37.0
5	35.305980041955124	37.0	35.0	37.0	33.0	37.0
6	35.49787788035418	37.0	35.0	37.0	33.0	37.0
7	35.49814650309416	37.0	35.0	37.0	33.0	37.0
8	35.520031135249674	37.0	35.0	37.0	33.0	37.0
9	37.30557273491684	39.0	37.0	39.0	34.0	39.0
10	37.139383966845706	39.0	37.0	39.0	33.0	39.0
11	37.19483769564169	39.0	37.0	39.0	33.0	39.0
12	37.12234079105024	39.0	37.0	39.0	33.0	39.0
13	37.212120757791	39.0	37.0	39.0	34.0	39.0
14	38.28955782198184	40.0	38.0	41.0	34.0	41.0
15	38.301765788457715	40.0	38.0	41.0	34.0	41.0
16	38.29514517497336	40.0	38.0	41.0	33.0	41.0
17	38.28625563639226	40.0	38.0	41.0	33.0	41.0
18	38.361759866263355	40.0	38.0	41.0	34.0	41.0
19	38.35358373970332	40.0	38.0	41.0	34.0	41.0
20	38.30996565377153	40.0	38.0	41.0	34.0	41.0
21	38.287728688533804	40.0	38.0	41.0	34.0	41.0
22	38.207351767100334	40.0	38.0	41.0	34.0	41.0
23	38.21228817847545	40.0	38.0	41.0	34.0	41.0
24	38.19128188020925	40.0	38.0	41.0	33.0	41.0
25	38.109815474918754	40.0	37.0	41.0	33.0	41.0
26	38.00423799225117	40.0	37.0	41.0	33.0	41.0
27	37.8941963744676	40.0	37.0	41.0	33.0	41.0
28	37.90022226969973	40.0	37.0	41.0	33.0	41.0
29	37.881842227244846	40.0	37.0	41.0	33.0	41.0
30	37.67514140176092	40.0	37.0	41.0	33.0	41.0
31	37.66841208977247	40.0	37.0	41.0	33.0	41.0
32	37.59503185365933	40.0	37.0	41.0	33.0	41.0
33	37.496790895313346	39.0	36.0	41.0	32.0	41.0
34	37.47877817883778	39.0	36.0	41.0	32.0	41.0
35	37.46373530539907	39.0	36.0	41.0	32.0	41.0
36	37.39103474728847	39.0	36.0	41.0	32.0	41.0
37	37.354383360882785	39.0	36.0	41.0	32.0	41.0
38	37.33467269880894	39.0	36.0	41.0	32.0	41.0
39	37.223480376171786	39.0	36.0	41.0	31.0	41.0
40	37.077834375964386	39.0	36.0	41.0	31.0	41.0
41	37.05193539560633	39.0	36.0	41.0	31.0	41.0
42	36.98623901926462	39.0	36.0	41.0	31.0	41.0
43	36.278366873693585	39.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	11.0
10	19.0
11	27.0
12	15.0
13	20.0
14	12.0
15	18.0
16	16.0
17	17.0
18	22.0
19	47.0
20	88.0
21	166.0
22	303.0
23	603.0
24	1021.0
25	1891.0
26	3054.0
27	4775.0
28	7152.0
29	10023.0
30	13482.0
31	17987.0
32	23208.0
33	30341.0
34	40213.0
35	53851.0
36	75253.0
37	110879.0
38	189293.0
39	216567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.06603871415916	23.198009942789604	15.968559894749863	24.767391448301368
2	12.806682834007388	22.025565388397247	42.649045014924184	22.518706762671183
3	13.407273304272103	30.023526354389606	35.564776187281275	21.004424154057016
4	10.242897427343795	20.14008363537774	37.83320152077953	31.783817416498934
5	11.630739936954868	38.59621504312332	35.81253381210651	13.9605112078153
6	25.598372770899786	41.616784048557	17.464351263588878	15.320491916954342
7	22.725233920430195	34.058239908843184	22.246210857606208	20.97031531312041
8	24.58922585425155	34.53139075363047	23.157279238960545	17.722104153157442
9	22.877911589384528	14.094697636994475	19.40593143998031	43.621459333640686
10	14.165289194244227	28.771119682050628	33.435659856143154	23.62793126756199
11	29.91820125215679	23.036836298803443	25.704072695560477	21.34088975347929
12	19.319597340759813	27.978120365476855	27.714995021108752	24.98728727265458
13	29.55162491769524	20.19405806499171	27.06242917417873	23.191887843134314
14	21.356257473022154	23.361432521343016	26.469834915708685	28.81247508992615
15	24.04523357059593	28.903556939899723	22.694498481344464	24.356711008159884
16	24.986787509417415	26.877017013190002	23.37230237175138	24.763893105641202
17	19.46715243653319	32.03407385751	24.391194671524367	24.10757903443244
18	24.658068240171218	24.331098142255104	25.752549729565615	25.258283888008055
19	24.913197372744662	26.912750084647396	26.615266017724103	21.558786524883836
20	21.419352581714413	25.697575773477315	27.825942459759688	25.05712918504858
21	25.228672916205948	27.21773060012819	25.506041512833296	22.047554970832568
22	21.70709126551296	32.23222998104648	26.690980148154814	19.369698605285745
23	23.351687138218267	27.029694682144335	26.415985426904005	23.202632752733393
24	27.229849858629475	27.486978044151584	25.607368509168783	19.67580358805016
25	22.32754732445504	29.738911190823348	27.030694208618666	20.902847276102946
26	26.122124643450167	29.428308338924435	25.532154141975237	18.91741287565016
27	21.46907902381247	30.25504167400694	28.962279120266775	19.313600181913817
28	19.560483221074016	29.136946371656432	28.59857642441893	22.703993982850623
29	27.28344946581557	25.32700133311844	26.486327102535174	20.903222098530822
30	20.99330442203006	27.871920677579	31.48658323119422	19.64819166919672
31	24.041235464698598	29.07834913209867	25.461437643916195	21.41897775928654
32	26.582656466498992	26.0561558961442	27.61954024280997	19.74164739454683
33	19.47015101595619	30.801907596276262	29.850233451902163	19.87770793586538
34	23.20975437886301	28.51074303548694	27.189618918037578	21.08988366761247
35	23.24473780546466	25.36360899024087	32.326185469633764	19.065467734660704
36	22.265326801427822	28.853580616183084	25.29164308408891	23.58944949830018
37	20.839127463364232	27.322555939123838	29.207787810524767	22.630528786987163
38	20.82188563168199	24.55199349308265	30.16508429131699	24.461036583918368
39	23.787605621836654	25.32662651069056	27.626536928130296	23.259230939342487
40	19.535120236787822	24.44779285813346	35.155220214423416	20.8618666906553
41	21.82478550786565	25.627858801892607	26.65824565612041	25.889110034121337
42	22.0694196124586	24.63707818421023	31.876523496993297	21.416978706337872
43	22.332919779254578	23.646297566527856	31.550927747979397	22.469854906238172
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	31.0
1	32.0
2	33.0
3	110.0
4	187.0
5	187.0
6	257.5
7	328.0
8	375.5
9	423.0
10	618.5
11	814.0
12	814.0
13	1257.0
14	1700.0
15	4193.0
16	6686.0
17	7378.0
18	8070.0
19	8070.0
20	7763.0
21	7456.0
22	8521.0
23	9586.0
24	12095.0
25	14604.0
26	14604.0
27	17244.0
28	19884.0
29	23076.5
30	26269.0
31	29869.5
32	33470.0
33	33470.0
34	37049.0
35	40628.0
36	43748.0
37	46868.0
38	49207.5
39	51547.0
40	51547.0
41	53044.0
42	54541.0
43	55296.0
44	56051.0
45	56531.0
46	57011.0
47	57011.0
48	58661.5
49	60312.0
50	77740.0
51	95168.0
52	71346.5
53	47525.0
54	47525.0
55	58724.0
56	69923.0
57	56315.5
58	42708.0
59	31003.0
60	19298.0
61	19298.0
62	16048.0
63	12798.0
64	10056.5
65	7315.0
66	5860.0
67	4405.0
68	4405.0
69	3442.0
70	2479.0
71	1841.5
72	1204.0
73	879.0
74	554.0
75	554.0
76	387.5
77	221.0
78	179.5
79	138.0
80	102.5
81	67.0
82	67.0
83	52.0
84	37.0
85	28.0
86	19.0
87	15.0
88	11.0
89	11.0
90	10.5
91	10.0
92	5.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	800379.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.259652028557625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94244805524359	31.379358369580345
2	12.596084038696539	10.142279206407832
3	3.598702834256399	4.3464757158404
4	1.4839841717487126	2.389787454819619
5	0.8203983349645432	1.6514475745240287
6	0.5227100943355034	1.262647590585714
7	0.35803700590002613	1.009011168961718
8	0.2824285175901687	0.9096379072897247
9	0.21647618327632046	0.7843730230057435
>10	1.77445978784122	14.708898888648022
>50	0.2536847324954573	7.1961967703816105
>100	0.14121365587245735	10.23816236834281
>500	0.004373874296934343	1.13905499407492
>1k	0.003436615519019841	3.2769213516601026
>5k	3.1241959263816736E-4	0.7698935091356652
>10k+	0.0012496783705526694	8.795854106741743
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	24092	3.0100739774531817	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	19185	2.3969894262593097	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	16316	2.0385342444017147	TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	10338	1.2916380864565413	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	6121	0.764762693673872	RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	4997	0.6243292240301157	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4410	0.5509889689759476	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4182	0.5225024644574633	No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	2429	0.30348122576929176	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1911	0.23876188655624397	No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA	1843	0.2302659115244153	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1543	0.19278366873693586	No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT	1456	0.18191381832856685	No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	1245	0.15555130756803964	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1022	0.12768950709601326	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1015	0.12681492143097206	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	841	0.10507522061423401	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	837	0.10457545737706762	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.498816185831962E-4	0.0
5	0.0	0.0	0.0	2.498816185831962E-4	0.0
6	0.0	0.0	0.0	2.498816185831962E-4	0.0
7	0.0	0.0	0.0	2.498816185831962E-4	0.0
8	0.0	0.0	0.0	2.498816185831962E-4	0.0
9	0.0	0.0	0.0	2.498816185831962E-4	0.0
10	0.0	0.0	0.0	2.498816185831962E-4	0.0
11	0.0	0.0	0.0	2.498816185831962E-4	0.0
12	0.0	0.0	0.0	2.498816185831962E-4	0.0
13	0.0	0.0	0.0	2.498816185831962E-4	0.0
14	0.0	0.0	0.0	2.498816185831962E-4	0.0
15	0.0	0.0	0.0	2.498816185831962E-4	0.0
16	0.0	0.0	0.0	3.748224278747943E-4	0.0
17	0.0	0.0	0.0	4.997632371663924E-4	0.0
18	0.0	0.0	0.0	4.997632371663924E-4	0.0
19	0.0	0.0	0.0	4.997632371663924E-4	0.0
20	0.0	0.0	0.0	7.496448557495886E-4	0.0
21	0.0	0.0	0.0	0.0019990529486655696	0.0
22	0.0	0.0	0.0	0.002373875376540364	0.0
23	0.0	0.0	0.0	0.0024988161858319623	0.0
24	0.0	0.0	0.0	0.003623283469456345	0.0
25	0.0	0.0	0.0	0.003623283469456345	0.0
26	0.0	0.0	0.0	0.0038731650880395414	0.0
27	0.0	0.0	0.0	0.005497395608830316	0.0
28	0.0	0.0	0.0	0.013118784975617801	0.0
29	0.0	0.0	0.0	0.0329843736529819	0.0
30	0.0	0.0	0.0	0.06059629250642508	0.0
31	0.0	0.0	0.0	0.11644483425976944	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2905	0.0	33.5611	1
CGTCTTC	690	0.0	33.514492	37
TTATACA	3005	0.0	32.44426	2
TATACAC	3210	0.0	30.833334	3
GATTACG	60	1.2696546E-9	30.833332	1
CGGATCG	50	2.7190436E-7	29.599998	37
GGTATCA	620	0.0	28.64516	1
TACGTTA	40	5.9379112E-5	27.75	19
CCGTCTT	2530	0.0	26.543478	37
GCCGTCT	2575	0.0	26.07961	36
GTATCAA	1980	0.0	24.573233	1
AATTACG	55	1.9017227E-5	23.545454	16
GTCAACC	80	2.723209E-8	23.125	15
TAGGACC	160	0.0	23.125	4
TGGGACG	40	0.0019306778	23.125	11
TGCCGTC	3045	0.0	22.054188	35
ATGCCGT	3215	0.0	20.888025	34
GGGTCGA	45	0.0038248086	20.555555	24
AACCACG	45	0.0038248086	20.555555	35
GGGACGA	45	0.0038248086	20.555555	12
>>END_MODULE