FastQCFastQC Report
Fri 10 Feb 2017
ERR1631069.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631069.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422823
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT92770.6520136376766471No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG60090.4223294113182033No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT43640.3067141872179463RNA PCR Primer, Index 41 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35330.2483091712742906No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC32400.22771630765035428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31340.2202663296840155No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA24490.17212260414682642No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC16120.11329589133715157No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14640.10289403530867858No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC14540.10219120719864663RNA PCR Primer, Index 41 (95% over 23bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT14360.10092611660058912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG1400.029.071431
CTTATAC14150.027.8480591
TTACGGG1750.026.4285723
TATACAC17400.025.4109173
CCGTCTT8500.025.02941337
ATTACGG1650.024.6666642
GCCGTCT8850.024.03954936
GTATCAA21600.023.7245351
TTATACA18200.022.4642872
GGTATCA8000.021.9687521
TGCCGTC10000.021.4635
ATGCCGT10600.020.07075534
GCGGGTA851.2457349E-619.58823622
TATGCCG11400.018.8245633
GTCCTAT1156.408118E-817.695651
TACACAT23550.017.5966035
TTACCGG852.723853E-517.41176431
ATACACA25400.016.6062984
TAGGACC2550.015.9607864
ACACATC26150.015.2103246
Produced by FastQC (version 0.11.5)