##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631069.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1422823
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.549061267634837	31.0	31.0	34.0	30.0	34.0
2	31.52057002171036	31.0	31.0	34.0	28.0	34.0
3	30.998408094330777	31.0	31.0	34.0	27.0	34.0
4	35.14526965054684	37.0	35.0	37.0	32.0	37.0
5	35.35140913521921	37.0	35.0	37.0	33.0	37.0
6	35.53220674672816	37.0	35.0	37.0	33.0	37.0
7	35.54850392494358	37.0	35.0	37.0	33.0	37.0
8	35.55715292766563	37.0	35.0	37.0	33.0	37.0
9	37.33095191742051	39.0	37.0	39.0	34.0	39.0
10	37.16963951243408	39.0	37.0	39.0	33.0	39.0
11	37.23650728165063	39.0	37.0	39.0	34.0	39.0
12	37.17589257412904	39.0	37.0	39.0	33.0	39.0
13	37.237216435213654	39.0	37.0	39.0	34.0	39.0
14	38.42386579356673	40.0	38.0	41.0	34.0	41.0
15	38.42992979450009	40.0	38.0	41.0	34.0	41.0
16	38.426824699909965	40.0	38.0	41.0	34.0	41.0
17	38.407210173015194	40.0	38.0	41.0	34.0	41.0
18	38.42956291822665	40.0	38.0	41.0	34.0	41.0
19	38.46246159922914	40.0	38.0	41.0	34.0	41.0
20	38.45550641225226	40.0	38.0	41.0	34.0	41.0
21	38.40640051503244	40.0	38.0	41.0	34.0	41.0
22	38.36732397494277	40.0	38.0	41.0	34.0	41.0
23	38.34158430106907	40.0	38.0	41.0	34.0	41.0
24	38.31323291793849	40.0	38.0	41.0	34.0	41.0
25	38.29073468730826	40.0	38.0	41.0	34.0	41.0
26	38.18211611704337	40.0	38.0	41.0	33.0	41.0
27	38.136482190687104	40.0	38.0	41.0	33.0	41.0
28	38.09671828470583	40.0	38.0	41.0	33.0	41.0
29	38.02527861863352	40.0	37.0	41.0	33.0	41.0
30	37.944254485624704	40.0	37.0	41.0	33.0	41.0
31	37.92979520291702	40.0	37.0	41.0	33.0	41.0
32	37.86200602604821	40.0	37.0	41.0	33.0	41.0
33	37.83810987030713	40.0	37.0	41.0	33.0	41.0
34	37.773841159441474	40.0	37.0	41.0	33.0	41.0
35	37.72849539261033	40.0	37.0	41.0	33.0	41.0
36	37.69718018333974	40.0	37.0	41.0	33.0	41.0
37	37.65120608817822	40.0	37.0	41.0	33.0	41.0
38	37.60179656921486	40.0	37.0	41.0	32.0	41.0
39	37.53859475142024	40.0	37.0	41.0	32.0	41.0
40	37.483501461531056	40.0	37.0	41.0	32.0	41.0
41	37.41497501797483	40.0	37.0	41.0	32.0	41.0
42	37.35057347259638	39.0	36.0	41.0	31.0	41.0
43	36.7463936132604	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	9.0
11	9.0
12	6.0
13	6.0
14	6.0
15	6.0
16	8.0
17	12.0
18	18.0
19	53.0
20	109.0
21	206.0
22	453.0
23	805.0
24	1632.0
25	2728.0
26	4720.0
27	7185.0
28	10847.0
29	15809.0
30	21959.0
31	29598.0
32	38558.0
33	50427.0
34	66637.0
35	91280.0
36	126891.0
37	189640.0
38	339269.0
39	423929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.60237991654619	20.739543850500027	14.262912533744535	24.39516369920925
2	14.565761166357305	22.0210806263323	39.9800959079239	23.4330622993865
3	15.469035853370377	27.245131685388834	32.91098049441146	24.374851966829326
4	12.683587487691723	18.415994118734375	38.72540716589484	30.17501122767906
5	13.563317433018723	37.28728028714745	35.32308656804114	13.826315711792683
6	29.223803663561803	39.37917787384657	17.683717510892077	13.713300951699544
7	26.24163370988521	32.05493585639253	23.143145703998318	18.560284729723936
8	25.158435026703952	35.22700996539977	21.3393373596013	18.275217648294976
9	25.529949965666848	15.174902289322004	20.148605975585156	39.146541769425994
10	15.224029974213238	28.357357169514408	33.386092296793066	23.032520559479288
11	33.19590701021842	23.145254188328416	24.863598634545546	18.79524016690762
12	22.236075745191076	25.274542230481234	30.520310678137758	21.969071346189935
13	28.85502975422804	21.365833979349507	26.27846190285088	23.500674363571576
14	22.06163380828114	21.047031148639007	27.58888491400547	29.30245012907438
15	23.86473932456813	28.341402971416684	23.764797167321586	24.029060536693603
16	23.767678762572718	27.692833191479195	24.450968251145785	24.088519794802306
17	22.50687541598639	28.106939513910024	25.954879840992167	23.43130522911142
18	23.895101498921512	26.442010004055316	26.64681411531863	23.01607438170454
19	24.563280183128892	26.467733512882486	26.241422861452197	22.72756344253642
20	23.971077217615967	25.965773676697662	26.522764953897994	23.54038415178838
21	23.983236143919516	26.169945242661946	26.714707310747716	23.132111302670815
22	22.872556881636015	27.800857871991102	26.52149986329993	22.80508538307295
23	23.446837730343127	26.25491716116481	26.75589303799559	23.54235207049647
24	23.783914091914454	26.508778674508353	26.42746146217766	23.279845771399536
25	23.261361392105695	27.231637385676223	26.37411680862623	23.132884413591853
26	23.95842631163539	26.30566135070912	26.323091487837914	23.41282084981758
27	23.27007646067009	27.5631614051783	26.113718993859393	23.053043140292225
28	22.824834852964845	26.411858678134948	27.016993680872464	23.746312788027744
29	23.51922902567642	26.705429979695296	25.986507105943605	23.788833888684678
30	22.766781251076207	26.958377816495798	26.861528102933395	23.413312829494604
31	24.23182644643782	25.749443184429825	26.779016082815644	23.23971428631671
32	22.819985339005626	27.325113524310474	26.72707708548428	23.12782405119962
33	22.74710206399531	26.727498782350303	27.267903316153873	23.25749583750052
34	23.409868971755447	26.452482142894794	26.685891358236407	23.45175752711335
35	22.86798849892081	26.950857555718454	27.383799671498142	22.797354273862595
36	23.518666763188396	26.188640470388798	26.242969083294266	24.04972368312854
37	23.68221486439283	26.38838421925988	26.61849014248434	23.310910773862947
38	23.17217250494264	25.94827325675787	27.01825877147052	23.86129546682897
39	23.709133181007054	25.789434103890645	26.675981481884957	23.825451233217343
40	22.92660436329747	25.975613270238114	27.986615341472554	23.111167024991865
41	23.455060819230503	25.7422040548965	26.61427317382415	24.188461952048847
42	23.425893452664177	25.769825199620755	27.438971678135648	23.36530966957942
43	23.48689893261495	25.37364099399574	27.610532019794448	23.52892805359486
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	16.5
2	31.0
3	97.5
4	164.0
5	164.0
6	228.0
7	292.0
8	307.0
9	322.0
10	514.0
11	706.0
12	706.0
13	1086.0
14	1466.0
15	3508.5
16	5551.0
17	6421.5
18	7292.0
19	7292.0
20	7550.5
21	7809.0
22	9616.0
23	11423.0
24	15369.5
25	19316.0
26	19316.0
27	24689.5
28	30063.0
29	36131.5
30	42200.0
31	48852.5
32	55505.0
33	55505.0
34	63209.0
35	70913.0
36	76533.0
37	82153.0
38	87739.0
39	93325.0
40	93325.0
41	96652.5
42	99980.0
43	102399.0
44	104818.0
45	106913.5
46	109009.0
47	109009.0
48	110571.0
49	112133.0
50	115614.0
51	119095.0
52	108411.0
53	97727.0
54	97727.0
55	96890.0
56	96053.0
57	86178.5
58	76304.0
59	66339.5
60	56375.0
61	56375.0
62	49729.5
63	43084.0
64	36324.5
65	29565.0
66	24903.0
67	20241.0
68	20241.0
69	16632.0
70	13023.0
71	10520.5
72	8018.0
73	6106.5
74	4195.0
75	4195.0
76	3247.5
77	2300.0
78	1730.0
79	1160.0
80	903.0
81	646.0
82	646.0
83	489.0
84	332.0
85	233.5
86	135.0
87	103.5
88	72.0
89	72.0
90	45.0
91	18.0
92	12.5
93	7.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1422823.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.480250277033804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9831194273938	42.915456020889515
2	14.06875847205098	15.892139991771579
3	4.413915957558644	7.4789723395411665
4	1.926226170583685	4.3517494481895564
5	1.0064752599581246	2.8422987290038764
6	0.5970370017692642	2.023247957074675
7	0.380175843355758	1.5030698747410924
8	0.2753224016764076	1.2440222522846025
9	0.20015854606836467	1.0174504299325595
>10	1.0358913216350383	10.379686227948657
>50	0.07323657000893595	2.8238712725860484
>100	0.03555204477881317	3.679050298243612
>500	0.0022532635429968477	0.8575532426913338
>1k	0.0016273570032755012	1.9106701979133156
>5k	2.5036261588853864E-4	1.0807617171885342
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	9277	0.6520136376766471	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	6009	0.4223294113182033	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	4364	0.3067141872179463	RNA PCR Primer, Index 41 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3533	0.2483091712742906	No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	3240	0.22771630765035428	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3134	0.2202663296840155	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2449	0.17212260414682642	No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	1612	0.11329589133715157	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1464	0.10289403530867858	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	1454	0.10219120719864663	RNA PCR Primer, Index 41 (95% over 23bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1436	0.10092611660058912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.028281100319575E-5	0.0
5	0.0	0.0	0.0	7.028281100319575E-5	0.0
6	0.0	0.0	0.0	7.028281100319575E-5	0.0
7	0.0	0.0	0.0	7.028281100319575E-5	0.0
8	0.0	0.0	0.0	7.028281100319575E-5	0.0
9	0.0	0.0	0.0	7.028281100319575E-5	0.0
10	0.0	7.028281100319575E-5	0.0	1.405656220063915E-4	0.0
11	0.0	7.028281100319575E-5	0.0	1.405656220063915E-4	0.0
12	0.0	7.028281100319575E-5	0.0	1.405656220063915E-4	0.0
13	0.0	7.028281100319575E-5	0.0	2.1084843300958728E-4	0.0
14	0.0	7.028281100319575E-5	0.0	2.1084843300958728E-4	0.0
15	0.0	7.028281100319575E-5	0.0	2.1084843300958728E-4	0.0
16	0.0	7.028281100319575E-5	0.0	2.1084843300958728E-4	0.0
17	0.0	7.028281100319575E-5	0.0	3.514140550159788E-4	0.0
18	0.0	1.405656220063915E-4	0.0	3.514140550159788E-4	0.0
19	0.0	1.405656220063915E-4	0.0	4.2169686601917455E-4	0.0
20	0.0	1.405656220063915E-4	0.0	5.62262488025566E-4	0.0
21	0.0	1.405656220063915E-4	0.0	9.136765430415449E-4	0.0
22	0.0	1.405656220063915E-4	0.0	0.0014056562200639151	0.0
23	0.0	2.1084843300958728E-4	0.0	0.001757070275079894	0.0
24	0.0	2.1084843300958728E-4	0.0	0.002249049952102264	0.0
25	0.0	2.1084843300958728E-4	0.0	0.0024598983851118516	0.0
26	0.0	2.1084843300958728E-4	0.0	0.0028113124401278303	0.0
27	0.0	2.1084843300958728E-4	0.0	0.003514140550159788	0.0
28	0.0	2.81131244012783E-4	0.0	0.011385815382517712	0.0
29	0.0	2.81131244012783E-4	0.0	0.027621144724255933	0.0
30	0.0	2.81131244012783E-4	0.0	0.048776270836217855	0.0
31	0.0	2.81131244012783E-4	0.0	0.10324544936369458	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	140	0.0	29.07143	1
CTTATAC	1415	0.0	27.848059	1
TTACGGG	175	0.0	26.428572	3
TATACAC	1740	0.0	25.410917	3
CCGTCTT	850	0.0	25.029413	37
ATTACGG	165	0.0	24.666664	2
GCCGTCT	885	0.0	24.039549	36
GTATCAA	2160	0.0	23.724535	1
TTATACA	1820	0.0	22.464287	2
GGTATCA	800	0.0	21.968752	1
TGCCGTC	1000	0.0	21.46	35
ATGCCGT	1060	0.0	20.070755	34
GCGGGTA	85	1.2457349E-6	19.588236	22
TATGCCG	1140	0.0	18.82456	33
GTCCTAT	115	6.408118E-8	17.69565	1
TACACAT	2355	0.0	17.596603	5
TTACCGG	85	2.723853E-5	17.411764	31
ATACACA	2540	0.0	16.606298	4
TAGGACC	255	0.0	15.960786	4
ACACATC	2615	0.0	15.210324	6
>>END_MODULE