FastQCFastQC Report
Fri 10 Feb 2017
ERR1631068.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631068.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022832
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT248032.4249339089899418No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG180421.7639260406401052No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT148501.4518513304237646RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC94690.9257629796486618No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC54560.5334209332519905RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC43570.42597415802399613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29610.2894903561875264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27380.26768814428958027No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG22270.21772881568038546No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA18190.17783956700611636No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT15860.15505967744458524No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14070.13755924726641325No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC13880.1357016597056017No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12720.1243605988080154No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC12420.12142756581726032No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC12210.11937444272373177No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT11840.11575703536846715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11470.11213962801320257No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT11260.11008650491967402No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT10720.10480704553631486No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT10600.10363383234001282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC27100.034.0645751
CGTCTTC6150.031.58536537
TTATACA29500.031.2305092
TATACAC31450.029.7647063
GATTACG702.0736479E-1029.0714281
GGTATCA6450.028.682171
CCGTCTT22000.026.82499937
GCCGTCT22350.026.3221536
GCAACGC509.089812E-625.8999983
TTACGGG809.786163E-1025.43753
ATTACGG851.9754225E-923.9411772
TGCCGTC26800.022.08955235
AACCACG855.1793904E-821.76470635
ATGCCGT27550.021.42105134
GTATCAA16100.021.2577651
CGCTAAG450.003825524820.55555525
TATGCCG29900.019.86120433
CGTGGGA2450.018.87755237
CCTACGT500.00703436718.49999834
TCGCCAT2250.018.08888813
Produced by FastQC (version 0.11.5)