##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631068.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1022832
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.502385533499147	31.0	31.0	34.0	28.0	34.0
2	31.51827181785474	31.0	31.0	34.0	30.0	34.0
3	30.988555305270072	31.0	31.0	34.0	27.0	34.0
4	35.12386393855491	37.0	35.0	37.0	32.0	37.0
5	35.31889205656452	37.0	35.0	37.0	33.0	37.0
6	35.50549748150234	37.0	35.0	37.0	33.0	37.0
7	35.51660781047132	37.0	35.0	37.0	33.0	37.0
8	35.53815191546608	37.0	35.0	37.0	33.0	37.0
9	37.318362155270854	39.0	37.0	39.0	34.0	39.0
10	37.16247340716755	39.0	37.0	39.0	33.0	39.0
11	37.21643632580913	39.0	37.0	39.0	33.0	39.0
12	37.1554996323932	39.0	37.0	39.0	33.0	39.0
13	37.22215476246343	39.0	37.0	39.0	34.0	39.0
14	38.361856101490766	40.0	38.0	41.0	34.0	41.0
15	38.37786850626496	40.0	38.0	41.0	34.0	41.0
16	38.36451147402506	40.0	38.0	41.0	34.0	41.0
17	38.34226148575719	40.0	38.0	41.0	34.0	41.0
18	38.38183494454612	40.0	38.0	41.0	34.0	41.0
19	38.403074991787506	40.0	38.0	41.0	34.0	41.0
20	38.40600998013359	40.0	38.0	41.0	34.0	41.0
21	38.36948687565504	40.0	38.0	41.0	34.0	41.0
22	38.28832105370188	40.0	38.0	41.0	34.0	41.0
23	38.30558879659612	40.0	38.0	41.0	34.0	41.0
24	38.23812317174277	40.0	38.0	41.0	34.0	41.0
25	38.19859664148169	40.0	38.0	41.0	33.0	41.0
26	38.09921570697827	40.0	37.0	41.0	33.0	41.0
27	38.01703896632096	40.0	37.0	41.0	33.0	41.0
28	38.04531242667417	40.0	37.0	41.0	33.0	41.0
29	37.953831127692524	40.0	37.0	41.0	33.0	41.0
30	37.879521759194084	40.0	37.0	41.0	33.0	41.0
31	37.87824197913245	40.0	37.0	41.0	33.0	41.0
32	37.750993320506204	40.0	37.0	41.0	33.0	41.0
33	37.74588788774696	40.0	37.0	41.0	33.0	41.0
34	37.67771246890985	40.0	37.0	41.0	33.0	41.0
35	37.662518380340074	40.0	37.0	41.0	33.0	41.0
36	37.64367266569681	40.0	37.0	41.0	33.0	41.0
37	37.58951225616719	40.0	37.0	41.0	32.0	41.0
38	37.56954416756613	40.0	37.0	41.0	32.0	41.0
39	37.485958593395594	40.0	37.0	41.0	32.0	41.0
40	37.373739773491636	39.0	37.0	41.0	32.0	41.0
41	37.34730630250129	39.0	37.0	41.0	31.0	41.0
42	37.29822101772334	39.0	36.0	41.0	31.0	41.0
43	36.60542884853035	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	12.0
10	21.0
11	25.0
12	14.0
13	11.0
14	19.0
15	14.0
16	18.0
17	15.0
18	18.0
19	37.0
20	95.0
21	187.0
22	296.0
23	682.0
24	1253.0
25	2082.0
26	3477.0
27	5436.0
28	8032.0
29	11673.0
30	16225.0
31	22148.0
32	28879.0
33	37029.0
34	49351.0
35	66619.0
36	92634.0
37	137762.0
38	241730.0
39	297031.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.445543354138316	22.46253539193142	15.349930389350352	24.74199086457991
2	13.182125705883275	22.004004567710044	42.09039216606442	22.723477560342264
3	14.21592206735808	28.838949113832967	35.43973985952727	21.505388959281678
4	11.406076462214713	20.04600951084831	37.21002080498068	31.337893221956293
5	12.012823220235582	38.701565848546	35.98567506687315	13.299935864345269
6	26.649537754000658	40.45258654402678	17.999436857665774	14.898438844306789
7	23.268923928856353	33.95601623727064	22.616910695011498	20.158149138861514
8	24.08645799114615	35.78720650116539	22.59921472930061	17.527120778387847
9	23.279873918688505	15.355503152032787	19.349805246609414	42.01481768266929
10	14.041504372174513	29.37588968667386	33.580490246687624	23.002115694464
11	30.01128240023777	23.34146761149436	26.007007993492575	20.64024199477529
12	19.73647676255729	26.932966508673957	28.85420088538489	24.47635584338386
13	28.501552552129773	20.86100161121279	27.582437780593487	23.055008056063947
14	21.3159150280789	23.006515243950133	26.330912603438293	29.346657124532673
15	23.87967916529792	28.823306271215603	22.903761321507343	24.39325324197913
16	24.993645095186697	27.71354435528024	23.14642091760915	24.146389631923913
17	20.556943857837844	31.563345691179002	24.53824283948879	23.34146761149436
18	23.403452375365653	26.402185305113647	25.725045755314657	24.469316564206046
19	23.245850735995745	29.20440502448105	25.839140738655033	21.710603500868178
20	22.801887308961785	26.977548610133432	25.89516166877845	24.325402412126333
21	24.90868490622116	26.903538411000046	24.961968338886543	23.22580834389225
22	20.607978631876986	33.25111064182583	25.322242557917622	20.81866816837956
23	24.59445930514493	26.03809814319458	25.72103727689396	23.64640527476653
24	24.940361662521312	29.153859089273702	25.17510206954808	20.730677178656904
25	21.807882428394887	29.361517825019163	25.372495189825894	23.458104556760055
26	24.566595491732755	29.630868021336838	25.086720008759993	20.715816478170414
27	20.80292755799584	32.30247000484928	25.806975143523083	21.0876272936318
28	22.829653354607597	26.61678555227056	27.19078010856133	23.362780984560512
29	21.86918281790167	30.896080685782216	25.494704897774024	21.740031598542085
30	22.19553162200635	29.058633284840525	27.05282979022948	21.69300530292365
31	26.352519279803527	26.48352808672392	25.386378212648804	21.777574420823754
32	21.765548985561654	28.877469613778217	26.65100427049604	22.705977130164094
33	23.159717333833903	27.39198617172713	28.345515197021605	21.102781297417366
34	23.092550878345612	28.394790151266285	26.253089461416927	22.25956950897117
35	20.887301140363228	26.08180033475683	30.023601138798945	23.007297386081
36	22.3582171852269	28.66414034758396	25.241486382905503	23.736156084283635
37	22.202473133417804	27.33811613246359	27.79078089070346	22.668629843415143
38	22.021309462355497	25.330161746992662	28.76484114693322	23.883687643718616
39	24.01391430850814	25.778426955746397	26.60612886573748	23.60152987000798
40	20.76313607708793	25.076552161058707	32.17654512177953	21.983766640073835
41	22.029619722495973	26.425160730207892	26.46055266162967	25.08466688566646
42	23.31223504935317	24.923154535642215	29.699696528853224	22.06491388615139
43	22.498122858885917	24.701808312606566	29.99769268071394	22.802376147793577
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	19.5
2	26.0
3	97.0
4	168.0
5	168.0
6	230.5
7	293.0
8	288.0
9	283.0
10	484.0
11	685.0
12	685.0
13	964.0
14	1243.0
15	3332.5
16	5422.0
17	6109.5
18	6797.0
19	6797.0
20	7307.0
21	7817.0
22	9796.5
23	11776.0
24	14828.0
25	17880.0
26	17880.0
27	22033.5
28	26187.0
29	32661.5
30	39136.0
31	42199.5
32	45263.0
33	45263.0
34	49165.5
35	53068.0
36	56048.0
37	59028.0
38	63249.5
39	67471.0
40	67471.0
41	70636.0
42	73801.0
43	69949.0
44	66097.0
45	67770.5
46	69444.0
47	69444.0
48	71531.5
49	73619.0
50	93415.0
51	113211.0
52	92274.5
53	71338.0
54	71338.0
55	74757.5
56	78177.0
57	65416.0
58	52655.0
59	40008.5
60	27362.0
61	27362.0
62	23699.5
63	20037.0
64	16840.5
65	13644.0
66	11170.5
67	8697.0
68	8697.0
69	6948.0
70	5199.0
71	4243.5
72	3288.0
73	2594.5
74	1901.0
75	1901.0
76	1435.0
77	969.0
78	744.0
79	519.0
80	354.0
81	189.0
82	189.0
83	131.5
84	74.0
85	56.0
86	38.0
87	25.5
88	13.0
89	13.0
90	8.5
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1022832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.38471202137131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.88393672213762	35.80124751246292
2	12.472729690568087	11.321424902536805
3	3.578477542629299	4.872245182415257
4	1.466978969835891	2.6631367234963945
5	0.7847088981929392	1.780689368254706
6	0.4640238477661938	1.2635753221150408
7	0.320477639844549	1.0181349775543527
8	0.2293726674008595	0.8328009964449431
9	0.18635627951030237	0.7611953471054325
>10	1.3091960241102376	11.851401056519267
>50	0.16666033205771	5.25262489153016
>100	0.12477644265681018	10.405608616381508
>500	0.0077715431302903815	2.3366007861197438
>1k	0.0034540191690179477	2.724385008161001
>5k	4.3175239612724346E-4	1.4622737528968583
>10k+	6.476285941908652E-4	5.652655556005644
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	24803	2.4249339089899418	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	18042	1.7639260406401052	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	14850	1.4518513304237646	RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	9469	0.9257629796486618	No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC	5456	0.5334209332519905	RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	4357	0.42597415802399613	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2961	0.2894903561875264	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2738	0.26768814428958027	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	2227	0.21772881568038546	No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA	1819	0.17783956700611636	No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT	1586	0.15505967744458524	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1407	0.13755924726641325	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1388	0.1357016597056017	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1272	0.1243605988080154	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1242	0.12142756581726032	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1221	0.11937444272373177	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1184	0.11575703536846715	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1147	0.11213962801320257	No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	1126	0.11008650491967402	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1072	0.10480704553631486	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1060	0.10363383234001282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	9.776776635850267E-5	0.0
9	0.0	0.0	0.0	9.776776635850267E-5	0.0
10	0.0	0.0	0.0	9.776776635850267E-5	0.0
11	0.0	0.0	0.0	9.776776635850267E-5	0.0
12	0.0	0.0	0.0	9.776776635850267E-5	0.0
13	0.0	0.0	0.0	9.776776635850267E-5	0.0
14	0.0	0.0	0.0	9.776776635850267E-5	0.0
15	0.0	0.0	0.0	1.9553553271700535E-4	0.0
16	0.0	0.0	0.0	1.9553553271700535E-4	0.0
17	0.0	0.0	0.0	1.9553553271700535E-4	0.0
18	0.0	0.0	0.0	1.9553553271700535E-4	0.0
19	0.0	0.0	0.0	1.9553553271700535E-4	0.0
20	0.0	0.0	0.0	2.93303299075508E-4	0.0
21	0.0	0.0	0.0	7.821421308680214E-4	0.0
22	0.0	0.0	0.0	0.00146651649537754	0.0
23	0.0	0.0	0.0	0.002053123093528556	0.0
24	0.0	0.0	0.0	0.0025419619253210695	0.0
25	0.0	0.0	0.0	0.0025419619253210695	0.0
26	0.0	0.0	0.0	0.0028352652243965773	0.0
27	0.0	0.0	0.0	0.003812942887981604	0.0
28	0.0	0.0	0.0	0.009679008869491764	0.0
29	0.0	0.0	0.0	0.022877657327889624	0.0
30	0.0	0.0	0.0	0.04116022963692962	0.0
31	0.0	0.0	0.0	0.08867536408716192	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2710	0.0	34.064575	1
CGTCTTC	615	0.0	31.585365	37
TTATACA	2950	0.0	31.230509	2
TATACAC	3145	0.0	29.764706	3
GATTACG	70	2.0736479E-10	29.071428	1
GGTATCA	645	0.0	28.68217	1
CCGTCTT	2200	0.0	26.824999	37
GCCGTCT	2235	0.0	26.32215	36
GCAACGC	50	9.089812E-6	25.899998	3
TTACGGG	80	9.786163E-10	25.4375	3
ATTACGG	85	1.9754225E-9	23.941177	2
TGCCGTC	2680	0.0	22.089552	35
AACCACG	85	5.1793904E-8	21.764706	35
ATGCCGT	2755	0.0	21.421051	34
GTATCAA	1610	0.0	21.257765	1
CGCTAAG	45	0.0038255248	20.555555	25
TATGCCG	2990	0.0	19.861204	33
CGTGGGA	245	0.0	18.877552	37
CCTACGT	50	0.007034367	18.499998	34
TCGCCAT	225	0.0	18.088888	13
>>END_MODULE