FastQCFastQC Report
Fri 10 Feb 2017
ERR1631067.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631067.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510395
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT93651.8348533978585213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT86891.702406959315824No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85431.6738016634175492No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG77941.5270525769257144No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT53201.0423299601289198TruSeq Adapter, Index 5 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA47810.9367254773263846No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC45050.8826497124775909No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA31880.6246142693404128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29730.5824900322299396No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT26180.5129360593266No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA25580.5011804582725145No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20840.40831120994523845No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC20680.4051763829974823No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC20600.4036089695236043No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT20440.4004741425758481No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG20420.4000822892073786No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC19370.3795099873627289Illumina Paired End PCR Primer 2 (95% over 23bp)
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC18220.35697841867573155No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG17260.33816945698919465No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG14600.28605295898274863No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG13500.26450102371692513No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT13480.2641091703484556No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG13250.25960285661105614No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC13020.2550965428736567No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG12480.24451650192497967No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG12340.24177352834569305No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG12250.2400101881875802No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG11930.23374053429206793No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11860.2323690475024246No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG11750.23021385397584224No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG11570.22668717365961658No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11050.2164989860794091No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG10950.2145397192370615No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10310.2020004114460369No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.20180448476180216No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG9770.1914203704973599No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT9710.19024481039195135No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9650.18906925028654278No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9360.18338737644373476No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT9300.1822118163383262No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA9290.18201588965409143No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8950.17535438239010961No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC8580.16810509507342353No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8220.16105173444097218No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA8010.15693727407204225No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.1551739339139294No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC7730.151451326913469No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC7570.14831649996571283No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG7480.1465531598076No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7340.14381018622831335No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA7330.1436142595440786No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG7280.1426346261229048No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7220.14145906601749625No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG7110.13930387249091392No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7080.13871609243820962No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA7000.13714867896433156No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA6920.13558126549045346No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG6840.1340138520165754No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG6830.13381792533234063No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT6760.1324464385426973No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6760.1324464385426973No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG6690.131074951753054No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6640.1300953183318802No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6630.12989939164764544No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6550.12833197817376737No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6450.12637271133141978No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA6150.12049491080437699No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA6080.11912342401473369No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT6060.11873157064626416No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5980.1171641571723861No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5970.11696823048815133No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG5870.11500896364580375No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5870.11500896364580375No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC5840.11442118359309945No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA5790.11344155017192566No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC5680.11128635664534331No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5680.11128635664534331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC5550.10873930975029145No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG5340.10462484938136149No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA5330.10442892269712674No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA5320.10423299601289197No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5290.1036452159601877No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5260.10305743590748341No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT5240.10266558253901391No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA5140.10070631569666631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC200.001840977137.03
GTGTATA200.001840977137.06
AGACAGA200.001840977137.015
GTTGTAT251.2326642E-436.9999961
TAGCAAC750.034.5333371
GTTTGTG554.984031E-1033.6363641
TCAGAGT850.032.647063
TTATCAA800.032.3752
CTTATAC11650.032.2360531
ATTACGG750.032.066672
GATTACG750.032.066671
CGTCTTC2950.031.98305137
GGTCGCT352.3857261E-531.71428528
TTATACA12050.031.3195022
ATTACTT601.2678356E-930.8333342
TGCGGAG303.596699E-430.8333347
AGCAACG850.030.4705892
TATACAC12500.030.1923
TGATACA250.005493516629.5999982
ATAAGAG250.005493516629.59999810
Produced by FastQC (version 0.11.5)