FastQCFastQC Report
Fri 10 Feb 2017
ERR1631066.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631066.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860367
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT154731.7984185818377507No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG115831.3462859454163165No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT87271.0143345804755413TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC58420.6790125609187707No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC33060.3842546262234604RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC24610.2860407244815294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18060.2099104219478432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16350.19003518266042282No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG14210.1651620761837681No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG12920.150168474616065No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA10850.12610897442602984No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT9170.10658242354716067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG603.45608E-1133.9166681
CTTATAC19950.032.548871
TTATACA21350.030.2412172
TACGGTA250.005495584629.612
TATACAC22150.029.2325063
CGTCTTC4700.028.34042737
GGTATCA3900.024.6666641
CCGTCTT13200.024.1060637
GCCGTCT13950.022.6774236
GTATCAA9800.022.2755091
TATCGTG450.003825038220.55555537
CGTATAC555.141648E-420.1818181
CACGATA656.8995534E-519.92307718
TGCCGTC16000.019.77187535
TAACGCT759.26133E-619.7333344
TTACGGG1052.2573658E-819.380953
ATACACA35200.018.8678974
TACACAT35550.018.5780585
CACGGTA609.234239E-418.52
TGGCGGA500.00703347918.511
Produced by FastQC (version 0.11.5)