##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631066.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	860367
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.450782049985644	31.0	31.0	34.0	28.0	34.0
2	31.450249719015257	31.0	31.0	34.0	28.0	34.0
3	30.912913907669633	31.0	31.0	34.0	27.0	34.0
4	35.07644528439608	37.0	35.0	37.0	32.0	37.0
5	35.2618591833485	37.0	35.0	37.0	33.0	37.0
6	35.44992892567939	37.0	35.0	37.0	33.0	37.0
7	35.45669231851059	37.0	35.0	37.0	33.0	37.0
8	35.47363973746087	37.0	35.0	37.0	33.0	37.0
9	37.24602408042149	39.0	37.0	39.0	34.0	39.0
10	37.063268349436925	39.0	37.0	39.0	33.0	39.0
11	37.128063954103304	39.0	37.0	39.0	33.0	39.0
12	37.060770578137	39.0	37.0	39.0	33.0	39.0
13	37.140288969707115	39.0	37.0	39.0	33.0	39.0
14	38.28201686024685	40.0	38.0	41.0	33.0	41.0
15	38.280323396875986	40.0	38.0	41.0	33.0	41.0
16	38.27613797367867	40.0	38.0	41.0	33.0	41.0
17	38.24769081101437	40.0	38.0	41.0	33.0	41.0
18	38.27079374267028	40.0	38.0	41.0	33.0	41.0
19	38.28518411329119	40.0	38.0	41.0	34.0	41.0
20	38.29569241963023	40.0	38.0	41.0	34.0	41.0
21	38.234548744896074	40.0	38.0	41.0	34.0	41.0
22	38.17563667597665	40.0	38.0	41.0	33.0	41.0
23	38.1695102206384	40.0	38.0	41.0	33.0	41.0
24	38.14097937275604	40.0	37.0	41.0	33.0	41.0
25	38.10905927354257	40.0	37.0	41.0	33.0	41.0
26	37.977453807503075	40.0	37.0	41.0	33.0	41.0
27	37.91112862301785	40.0	37.0	41.0	33.0	41.0
28	37.86834339299392	40.0	37.0	41.0	33.0	41.0
29	37.80003649605343	40.0	37.0	41.0	33.0	41.0
30	37.73828842807779	40.0	37.0	41.0	33.0	41.0
31	37.67016633599383	40.0	37.0	41.0	32.0	41.0
32	37.61575932131288	40.0	37.0	41.0	32.0	41.0
33	37.57530216756338	40.0	37.0	41.0	32.0	41.0
34	37.498739491403086	40.0	37.0	41.0	32.0	41.0
35	37.50297838015638	40.0	37.0	41.0	32.0	41.0
36	37.49920557157585	39.0	37.0	41.0	32.0	41.0
37	37.390455468422196	39.0	36.0	41.0	31.0	41.0
38	37.36696781722218	39.0	36.0	41.0	31.0	41.0
39	37.3127153877357	39.0	36.0	41.0	31.0	41.0
40	37.22280491929607	39.0	36.0	41.0	31.0	41.0
41	37.172879712959706	39.0	36.0	41.0	31.0	41.0
42	37.09991898805975	39.0	36.0	41.0	31.0	41.0
43	36.39294161677517	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	8.0
10	20.0
11	9.0
12	18.0
13	14.0
14	12.0
15	12.0
16	11.0
17	6.0
18	21.0
19	31.0
20	77.0
21	177.0
22	361.0
23	664.0
24	1202.0
25	2013.0
26	3239.0
27	4961.0
28	7467.0
29	10608.0
30	14561.0
31	19171.0
32	25188.0
33	32784.0
34	43008.0
35	58029.0
36	80909.0
37	120328.0
38	210774.0
39	224677.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.38106296499052	22.097430515117384	14.734293621210485	24.78721289868161
2	14.261588368684528	21.57846593372363	40.95566194426332	23.20428375332852
3	14.806820810189139	28.135318997590563	33.549985064513166	23.507875127707127
4	11.699309713180538	19.30873685299413	38.072125035014125	30.919828398811205
5	12.597995971486586	37.94682966687471	36.16538058758646	13.289793774052235
6	28.227372737448086	39.985959480082336	17.585170049525377	14.201497732944196
7	25.202384563796613	32.57877161722846	22.870821405284026	19.348022413690902
8	24.570444938032256	35.63897732014361	21.948656794135527	17.841920947688603
9	24.055083470193534	14.877372098186006	19.586176596731395	41.48136783488906
10	14.348063094005234	28.796664679142737	33.96620279485382	22.889069431998205
11	31.69868207404515	23.05957806377976	25.4153169519519	19.826422910223197
12	20.742543588956806	25.948461528626737	29.38280989391736	23.926184988499095
13	28.52329296683857	20.705582617650375	26.929089562942327	23.84203485256873
14	21.57730363902846	21.90239746526773	26.383740891968195	30.136558003735615
15	23.67722146479351	28.89197284414674	22.845948298807368	24.584857392252378
16	24.988638569354706	27.15968883046421	23.50752643929858	24.344146160882506
17	21.404470417856565	30.164569305889234	24.944936288816283	23.486023987437918
18	23.661995404286778	25.80549928112073	25.846295824921228	24.686209489671267
19	24.04322806430279	27.05856919198435	25.497839875309026	23.400362868403832
20	23.545649705300182	26.696281935499616	25.392187287518002	24.3658810716822
21	24.33008239507094	26.814254847059455	25.228536194437957	23.627126563431652
22	23.576218055783173	29.156511116767614	25.6548658886266	21.612404938822618
23	23.975117595165784	25.67671702889581	25.817238457541958	24.53092691839645
24	23.657229996036573	27.42108890740812	25.56955345800106	23.352127638554247
25	23.291107167057778	27.64541178357608	26.74591191898341	22.317569130382733
26	25.142642616464833	26.68349669385274	25.199595056528203	22.97426563315422
27	23.849008620739752	28.427636113426015	25.612790820661413	22.110564445172816
28	22.516321523256934	26.641537855357072	27.087510329894105	23.75463029149189
29	23.679894742592406	25.729485208056563	26.16243998200768	24.428180067343355
30	25.39300089380462	25.687177681152345	27.15527211062256	21.764549314420474
31	23.355614522639755	26.985693314597142	25.57304034208657	24.085651820676528
32	24.240818162481826	26.01447986731244	27.900070551287996	21.84463141891774
33	24.938892356401396	25.51515806626707	27.506401338033655	22.039548239297883
34	22.177047701736583	27.375526955357422	26.355264671936514	24.09216067096948
35	22.29257979443656	25.510973805364458	30.77767975759182	21.418766642607167
36	25.176814080502858	25.681714896085044	25.007467743416473	24.134003279995632
37	22.51585660537887	26.811697798730076	27.6931820955476	22.979263500343457
38	22.416364179472247	25.18309047185678	27.96318315323577	24.437362195435202
39	23.789266673407976	25.750057824161082	26.649790147692787	23.810885354738154
40	21.74351178043788	25.216448329608177	30.816035482532456	22.224004407421486
41	22.61360558924273	26.114902128975192	26.257631917542163	25.01386036423991
42	22.94021039858572	25.76725978564961	28.635338175453036	22.657191640311638
43	23.441624330082394	24.551964452379043	28.97926117575407	23.027150041784495
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	13.0
2	15.0
3	60.0
4	105.0
5	105.0
6	133.0
7	161.0
8	190.0
9	219.0
10	297.5
11	376.0
12	376.0
13	608.0
14	840.0
15	1874.5
16	2909.0
17	3453.0
18	3997.0
19	3997.0
20	4413.5
21	4830.0
22	6198.0
23	7566.0
24	9929.0
25	12292.0
26	12292.0
27	15731.0
28	19170.0
29	22843.5
30	26517.0
31	30762.0
32	35007.0
33	35007.0
34	41349.0
35	47691.0
36	50461.5
37	53232.0
38	55948.5
39	58665.0
40	58665.0
41	60508.5
42	62352.0
43	63082.5
44	63813.0
45	63144.0
46	62475.0
47	62475.0
48	60082.0
49	57689.0
50	56341.5
51	54994.0
52	54821.0
53	54648.0
54	54648.0
55	65898.5
56	77149.0
57	57368.5
58	37588.0
59	40907.5
60	44227.0
61	44227.0
62	37309.0
63	30391.0
64	21981.5
65	13572.0
66	11304.5
67	9037.0
68	9037.0
69	7515.5
70	5994.0
71	5597.5
72	5201.0
73	4724.5
74	4248.0
75	4248.0
76	3086.5
77	1925.0
78	1397.5
79	870.0
80	605.5
81	341.0
82	341.0
83	249.5
84	158.0
85	109.0
86	60.0
87	40.0
88	20.0
89	20.0
90	14.5
91	9.0
92	6.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	860367.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.622280674387156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.77120864893463	45.21152190580369
2	10.276823368034187	11.226870608997283
3	2.7792101441282426	4.554203896370306
4	1.1121118746043328	2.4298434782382694
5	0.6239048005213252	1.703955156408668
6	0.3990026558880394	1.307666103584544
7	0.2777761816409651	1.0620937990776644
8	0.20636999436076672	0.9017919803796397
9	0.16733296817380602	0.8226097518301134
>10	1.1886768920255635	13.083178140879484
>50	0.1294513904571707	4.9006710331047465
>100	0.06429870791556369	5.8830705583903
>500	0.0014903674004549216	0.5591516244550732
>1k	0.0014903674004549216	1.5125470107451502
>5k	4.2581925727283473E-4	1.6943178071310234
>10k+	4.2581925727283473E-4	3.146507144604089
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	15473	1.7984185818377507	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	11583	1.3462859454163165	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	8727	1.0143345804755413	TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	5842	0.6790125609187707	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	3306	0.3842546262234604	RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	2461	0.2860407244815294	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1806	0.2099104219478432	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1635	0.19003518266042282	No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG	1421	0.1651620761837681	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1292	0.150168474616065	No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	1085	0.12610897442602984	No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT	917	0.10658242354716067	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.3245893903415634E-4	0.0
10	2.3245893903415634E-4	0.0	0.0	2.3245893903415634E-4	0.0
11	2.3245893903415634E-4	0.0	0.0	2.3245893903415634E-4	0.0
12	2.3245893903415634E-4	0.0	0.0	2.3245893903415634E-4	0.0
13	2.3245893903415634E-4	0.0	0.0	2.3245893903415634E-4	0.0
14	2.3245893903415634E-4	0.0	0.0	2.3245893903415634E-4	0.0
15	2.3245893903415634E-4	0.0	0.0	2.3245893903415634E-4	0.0
16	2.3245893903415634E-4	0.0	0.0	2.3245893903415634E-4	0.0
17	2.3245893903415634E-4	0.0	0.0	2.3245893903415634E-4	0.0
18	2.3245893903415634E-4	0.0	0.0	2.3245893903415634E-4	0.0
19	2.3245893903415634E-4	0.0	0.0	2.3245893903415634E-4	0.0
20	2.3245893903415634E-4	0.0	0.0	3.4868840855123456E-4	0.0
21	2.3245893903415634E-4	0.0	0.0	6.973768171024691E-4	0.0
22	2.3245893903415634E-4	0.0	0.0	9.298357561366254E-4	0.0
23	2.3245893903415634E-4	0.0	0.0	0.0013947536342049382	0.0
24	2.3245893903415634E-4	0.0	0.0	0.0017434420427561727	0.0
25	2.3245893903415634E-4	0.0	0.0	0.0017434420427561727	0.0
26	2.3245893903415634E-4	0.0	0.0	0.001975900981790329	0.0
27	2.3245893903415634E-4	0.0	0.0	0.002673277798892798	0.0
28	2.3245893903415634E-4	0.0	0.0	0.007554915518610082	0.0
29	2.3245893903415634E-4	0.0	0.0	0.016155896262873868	0.0
30	2.3245893903415634E-4	0.0	0.0	0.02870867897071831	0.0
31	2.3245893903415634E-4	0.0	0.0	0.06636702709425164	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	60	3.45608E-11	33.916668	1
CTTATAC	1995	0.0	32.54887	1
TTATACA	2135	0.0	30.241217	2
TACGGTA	25	0.0054955846	29.6	12
TATACAC	2215	0.0	29.232506	3
CGTCTTC	470	0.0	28.340427	37
GGTATCA	390	0.0	24.666664	1
CCGTCTT	1320	0.0	24.10606	37
GCCGTCT	1395	0.0	22.67742	36
GTATCAA	980	0.0	22.275509	1
TATCGTG	45	0.0038250382	20.555555	37
CGTATAC	55	5.141648E-4	20.181818	1
CACGATA	65	6.8995534E-5	19.923077	18
TGCCGTC	1600	0.0	19.771875	35
TAACGCT	75	9.26133E-6	19.733334	4
TTACGGG	105	2.2573658E-8	19.38095	3
ATACACA	3520	0.0	18.867897	4
TACACAT	3555	0.0	18.578058	5
CACGGTA	60	9.234239E-4	18.5	2
TGGCGGA	50	0.007033479	18.5	11
>>END_MODULE