FastQCFastQC Report
Fri 10 Feb 2017
ERR1631065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652950
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT146222.239375143579141No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG114061.746841258901907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT98791.512979554330347No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT97681.4959797840569722No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT70081.0732827934757638TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC66091.0121755111417414No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA55660.8524389310054368No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA36160.5537943180948005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33760.5170380580442607No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT31220.47813768282410596No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC29230.44766061719886663No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA26750.4096791484799755No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT25400.38900375220154687No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC24540.37583275901677005No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT24220.37093192434336475No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG23330.3573014779079562No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC23290.35668887357378054RNA PCR Primer, Index 7 (95% over 22bp)
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC21150.3239145416953825No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG19810.30339229650049776No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG17290.2647982234474309No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG16260.2490236618424075No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT16030.24550118692089748No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG15320.23462745998927942No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG14960.22911402098169847No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG14850.22742935906271536No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC14360.2199249559690635No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG13790.21119534420706026No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG13750.21058273987288462No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA13560.2076728692855502No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG13330.20415039436404012No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG12810.1961865380197565No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG12470.19097940117926335No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12430.19036679684508767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.18041197641473314No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG11390.1744390841565204No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA11300.17306072440462517No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11270.17260127115399343No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA10940.16754728539704417No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT10800.16540317022742937No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC10340.15835822038440922No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG10220.15652040738188222No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT10210.1563672562983383No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9960.1525384792097404No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT9190.14074584577685886No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9180.14059269469331495No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG8980.13752967302243663No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA8900.1363044643540853No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC8330.1275748525920821No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.1275748525920821No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG8250.12634964392373077No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8250.12634964392373077No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA8250.12634964392373077No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8220.125890190673099No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC8040.12313347116930853No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG7870.12052990274906196No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7750.11869208974653496No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA7700.11792633432881539No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7560.11578221915920055No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT7540.11547591699211272No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG7440.11394440615667355No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7420.11363810398958574No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG7410.11348495290604181No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7350.11256604640477831No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7160.1096561758174439No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7090.10858411823263649No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT6960.10659315414656559No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA6760.10353013247568726No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6730.10307067922505553No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG6720.1029175281415116No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6680.10230492380733594No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA6630.10153916838961635No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6610.10123286622252853No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT6600.10107971513898462No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6590.10092656405544069No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA6590.10092656405544069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAC251.2330417E-437.024
CCTTTAT308.306488E-637.01
GATTACG700.037.01
CGTCTTC3350.033.68656537
CTTATAC18600.032.822581
TTATACA18850.032.3872682
GTACTAG352.38674E-531.7142851
TATACAC19400.031.6597943
TAGAACC303.5977917E-430.83333225
CTTTATA303.5977917E-430.8333322
CGGCGTA250.005494626229.612
TTACTCG250.005494626229.629
TCGGCGT250.005494626229.611
GCAGCAT250.005494626229.625
TATCCGG250.005494626229.610
TCAGAGT1400.029.0714283
GATACAC454.0047707E-628.7777793
TGCTTTG358.865276E-426.428577
CGCGGAT358.865276E-426.4285718
CGACGAG358.865276E-426.4285724
Produced by FastQC (version 0.11.5)