##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1631065.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 652950 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39392296500498 31.0 31.0 34.0 28.0 34.0 2 31.33070526073972 31.0 31.0 34.0 28.0 34.0 3 30.76614748449345 31.0 31.0 34.0 26.0 34.0 4 34.96208132322536 37.0 35.0 37.0 32.0 37.0 5 35.205089210506166 37.0 35.0 37.0 32.0 37.0 6 35.424239221992494 37.0 35.0 37.0 33.0 37.0 7 35.43434872501723 37.0 35.0 37.0 33.0 37.0 8 35.44125430737422 37.0 35.0 37.0 33.0 37.0 9 37.22784133547745 39.0 37.0 39.0 34.0 39.0 10 37.054217015085385 39.0 37.0 39.0 33.0 39.0 11 37.12718584883988 39.0 37.0 39.0 33.0 39.0 12 37.01690328509074 39.0 37.0 39.0 33.0 39.0 13 37.088614748449345 39.0 37.0 39.0 33.0 39.0 14 38.06897005896317 40.0 37.0 41.0 33.0 41.0 15 38.07965234704036 40.0 37.0 41.0 33.0 41.0 16 38.09362891492457 40.0 37.0 41.0 33.0 41.0 17 38.14208898077954 40.0 37.0 41.0 33.0 41.0 18 38.215374837276975 40.0 38.0 41.0 33.0 41.0 19 38.21300252699288 40.0 38.0 41.0 33.0 41.0 20 38.20657018148403 40.0 38.0 41.0 34.0 41.0 21 38.134122061413585 40.0 37.0 41.0 33.0 41.0 22 38.01640248104756 40.0 37.0 41.0 33.0 41.0 23 38.041367639176045 40.0 37.0 41.0 33.0 41.0 24 37.95970748143043 40.0 37.0 41.0 33.0 41.0 25 37.89092732981086 40.0 37.0 41.0 33.0 41.0 26 37.76004900834673 40.0 37.0 41.0 33.0 41.0 27 37.618100926564054 39.0 36.0 41.0 32.0 41.0 28 37.60423922199249 39.0 36.0 41.0 32.0 41.0 29 37.48992572172448 39.0 36.0 41.0 32.0 41.0 30 37.229777165173445 39.0 36.0 41.0 31.0 41.0 31 37.14455624473543 39.0 35.0 41.0 31.0 41.0 32 36.93341144038594 39.0 35.0 40.0 31.0 41.0 33 36.910205988207366 39.0 35.0 40.0 31.0 41.0 34 36.80487939352171 39.0 35.0 40.0 31.0 41.0 35 36.68330653189371 39.0 35.0 40.0 31.0 41.0 36 36.55460448732675 39.0 35.0 40.0 30.0 41.0 37 36.41532889195191 39.0 35.0 40.0 30.0 41.0 38 36.297927865839654 39.0 35.0 40.0 30.0 41.0 39 36.12108124664982 38.0 35.0 40.0 30.0 41.0 40 35.91718967761697 38.0 35.0 40.0 29.0 41.0 41 35.748951680833144 38.0 35.0 40.0 28.0 41.0 42 35.518943257523546 38.0 34.0 40.0 27.0 41.0 43 34.690124818133086 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 3.0 9 7.0 10 20.0 11 9.0 12 5.0 13 7.0 14 15.0 15 15.0 16 15.0 17 19.0 18 24.0 19 55.0 20 113.0 21 260.0 22 473.0 23 834.0 24 1400.0 25 2292.0 26 3599.0 27 5355.0 28 7754.0 29 10495.0 30 13703.0 31 17714.0 32 22348.0 33 28204.0 34 37651.0 35 49723.0 36 66558.0 37 98937.0 38 145148.0 39 140194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.43778237231028 20.588712765142812 15.323991117237155 22.64951374530975 2 14.932077494448274 23.11723715445287 40.24994256834367 21.70074278275519 3 15.837200398192817 29.899992342445824 32.79286315950685 21.46994409985451 4 11.230109503024734 18.86714143502565 36.26755494295122 33.63519411899839 5 14.216555632131097 37.669499961712226 31.548051152461902 16.565893253694767 6 27.019373612068303 39.675319702886895 16.190213645761546 17.115093039283252 7 25.507006662072136 31.44467417106976 19.869208974653496 23.17911019220461 8 26.958266329734283 30.965004977410217 21.983000229726628 20.093728463128876 9 25.160425760012252 12.415805191821732 18.379202082854736 44.044566965311276 10 17.205911631824794 27.4134313500268 29.23929856803737 26.141358450111035 11 34.248104755341146 22.53143425989739 22.483344819664598 20.737116165096868 12 23.281721418179032 26.096332031549125 26.030630216708783 24.59131633356306 13 32.596217168236464 20.437552645684967 25.133930622559152 21.83229956351941 14 22.925798300022972 21.885289838425606 27.832912167853586 27.355999693697836 15 26.89792480281798 25.38617045715598 23.953595221686193 23.76230951833984 16 23.74852592082089 24.47201163948235 22.742476453020906 29.036985986675855 17 22.2151772723792 27.199019833065318 23.602419787119995 26.983383107435483 18 25.307450800214408 21.381575924649667 27.607933226127574 25.703040049008347 19 26.948617811471014 23.62416724098323 28.002297266253155 21.424917681292595 20 24.845240830078875 22.20920438012099 27.86859637031932 25.076958419480817 21 27.0887510529137 22.30553641167011 27.927865839650817 22.67784669576537 22 22.11838578757945 28.58672180105674 27.061949613293514 22.232942798070297 23 25.44467417106976 22.180871429665363 28.3864001837813 23.988054215483576 24 23.995711769660772 24.25897848227276 27.994792863159507 23.75051688490696 25 22.69285550195268 27.037292288842945 28.264185619113256 22.005666590091124 26 25.834903131939658 23.46351175434566 28.421778084079946 22.279807029634735 27 22.726855042499423 27.660157745616047 28.20767286928555 21.405314342598974 28 22.66130637874263 23.95818975419251 28.95550961023049 24.424994256834367 29 23.19871353089823 23.631824795160426 27.939811624167245 25.2296500497741 30 23.098399571176966 25.362585190290222 30.56788421778084 20.971131020751972 31 27.26181177731832 21.342369247262425 25.82678612451183 25.56903285090742 32 20.53847920974041 23.122137989126273 30.56114557010491 25.778237231028413 33 23.127957730300942 22.598208132322537 30.364959032085153 23.90887510529137 34 19.950072746764683 25.089669959414962 28.39666130637874 26.563595987441612 35 20.94050080404319 24.81139444061567 33.191515429971666 21.056589325369476 36 23.494754575388622 23.741480970977868 26.817214181790337 25.946550271843172 37 23.343747607014322 25.478061107282336 28.0477831380657 23.13040814763764 38 22.173520177655256 22.771881461061337 28.836970671567503 26.217627689715904 39 24.193889271766597 22.714296653648823 28.287158281644842 24.804655792939734 40 21.37070219771805 24.726701891415882 31.909334558542003 21.99326135232407 41 24.40676927789264 22.472164790565895 27.04265257676698 26.078413354774483 42 23.767976108430965 23.23883911478674 29.57730300941879 23.4158817673635 43 24.521479439467033 22.72317941649437 29.083390764989662 23.67195037904893 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 10.0 2 18.0 3 147.5 4 277.0 5 277.0 6 411.5 7 546.0 8 622.5 9 699.0 10 1130.5 11 1562.0 12 1562.0 13 2438.0 14 3314.0 15 8922.0 16 14530.0 17 14818.5 18 15107.0 19 15107.0 20 12001.0 21 8895.0 22 6944.5 23 4994.0 24 4968.0 25 4942.0 26 4942.0 27 5135.0 28 5328.0 29 5844.5 30 6361.0 31 7904.0 32 9447.0 33 9447.0 34 12129.5 35 14812.0 36 15953.5 37 17095.0 38 19424.0 39 21753.0 40 21753.0 41 24606.5 42 27460.0 43 32304.5 44 37149.0 45 43090.0 46 49031.0 47 49031.0 48 57950.5 49 66870.0 50 65027.5 51 63185.0 52 77484.0 53 91783.0 54 91783.0 55 75751.0 56 59719.0 57 58842.5 58 57966.0 59 44854.5 60 31743.0 61 31743.0 62 24408.0 63 17073.0 64 13326.0 65 9579.0 66 7912.5 67 6246.0 68 6246.0 69 4882.5 70 3519.0 71 2431.0 72 1343.0 73 833.0 74 323.0 75 323.0 76 233.0 77 143.0 78 102.0 79 61.0 80 47.5 81 34.0 82 34.0 83 28.0 84 22.0 85 16.5 86 11.0 87 8.5 88 6.0 89 6.0 90 4.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 652950.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.934652891399885 #Duplication Level Percentage of deduplicated Percentage of total 1 67.54329583811675 11.438222701596414 2 11.997411922119177 4.0634401299246194 3 5.078801978886604 2.5802324584979845 4 3.0020562852888957 2.033551246072512 5 1.9265017651616965 1.6312319343841253 6 1.3635384572873455 1.3854630286941652 7 1.0620437519689183 1.2589739606551538 8 0.8525492629857023 1.155010067318533 9 0.6266601004953742 0.9551044154501038 >10 5.072485561008939 17.526906244024893 >50 0.7487130778452384 8.866490870591855 >100 0.6321251533145931 21.20711231907488 >500 0.0555614327841357 6.356565338316174 >1k 0.031879510613848354 9.537470225643428 >5k 0.0045542158019783365 5.989454794632784 >10k+ 0.0018216863207913346 4.014770265122382 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT 14622 2.239375143579141 No Hit ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG 11406 1.746841258901907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 9879 1.512979554330347 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 9768 1.4959797840569722 No Hit TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT 7008 1.0732827934757638 TruSeq Adapter, Index 7 (95% over 21bp) ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC 6609 1.0121755111417414 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 5566 0.8524389310054368 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 3616 0.5537943180948005 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3376 0.5170380580442607 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 3122 0.47813768282410596 No Hit CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC 2923 0.44766061719886663 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 2675 0.4096791484799755 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 2540 0.38900375220154687 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC 2454 0.37583275901677005 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 2422 0.37093192434336475 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG 2333 0.3573014779079562 No Hit CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC 2329 0.35668887357378054 RNA PCR Primer, Index 7 (95% over 22bp) TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 2115 0.3239145416953825 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG 1981 0.30339229650049776 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG 1729 0.2647982234474309 No Hit GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG 1626 0.2490236618424075 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 1603 0.24550118692089748 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG 1532 0.23462745998927942 No Hit GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG 1496 0.22911402098169847 No Hit CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG 1485 0.22742935906271536 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 1436 0.2199249559690635 No Hit GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG 1379 0.21119534420706026 No Hit GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG 1375 0.21058273987288462 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA 1356 0.2076728692855502 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 1333 0.20415039436404012 No Hit GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG 1281 0.1961865380197565 No Hit GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG 1247 0.19097940117926335 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 1243 0.19036679684508767 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1178 0.18041197641473314 No Hit GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG 1139 0.1744390841565204 No Hit TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA 1130 0.17306072440462517 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 1127 0.17260127115399343 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA 1094 0.16754728539704417 No Hit AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT 1080 0.16540317022742937 No Hit GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC 1034 0.15835822038440922 No Hit TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG 1022 0.15652040738188222 No Hit CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT 1021 0.1563672562983383 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 996 0.1525384792097404 No Hit TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT 919 0.14074584577685886 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC 918 0.14059269469331495 No Hit GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG 898 0.13752967302243663 No Hit GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA 890 0.1363044643540853 No Hit ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC 833 0.1275748525920821 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 833 0.1275748525920821 No Hit GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG 825 0.12634964392373077 No Hit GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 825 0.12634964392373077 No Hit TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA 825 0.12634964392373077 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 822 0.125890190673099 No Hit CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC 804 0.12313347116930853 No Hit GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG 787 0.12052990274906196 No Hit CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG 775 0.11869208974653496 No Hit GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA 770 0.11792633432881539 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC 756 0.11578221915920055 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT 754 0.11547591699211272 No Hit CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG 744 0.11394440615667355 No Hit GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC 742 0.11363810398958574 No Hit GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG 741 0.11348495290604181 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT 735 0.11256604640477831 No Hit ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG 716 0.1096561758174439 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC 709 0.10858411823263649 No Hit GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT 696 0.10659315414656559 No Hit CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA 676 0.10353013247568726 No Hit TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA 673 0.10307067922505553 No Hit CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG 672 0.1029175281415116 No Hit CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA 668 0.10230492380733594 No Hit TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA 663 0.10153916838961635 No Hit GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC 661 0.10123286622252853 No Hit ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT 660 0.10107971513898462 No Hit GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA 659 0.10092656405544069 No Hit AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA 659 0.10092656405544069 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.0630216708783217E-4 0.0 10 0.0 0.0 0.0 3.0630216708783217E-4 0.0 11 0.0 0.0 0.0 4.5945325063174823E-4 0.0 12 0.0 0.0 0.0 4.5945325063174823E-4 0.0 13 0.0 0.0 0.0 4.5945325063174823E-4 0.0 14 0.0 0.0 0.0 4.5945325063174823E-4 0.0 15 0.0 0.0 0.0 4.5945325063174823E-4 0.0 16 0.0 0.0 0.0 4.5945325063174823E-4 0.0 17 0.0 0.0 0.0 9.189065012634965E-4 0.0 18 0.0 0.0 0.0 9.189065012634965E-4 0.0 19 0.0 0.0 0.0 9.189065012634965E-4 0.0 20 0.0 0.0 0.0 0.0012252086683513287 0.0 21 0.0 0.0 0.0 0.0015315108354391608 0.0 22 0.0 0.0 0.0 0.002297266253158741 0.0 23 0.0 0.0 0.0 0.0032161727544222376 0.0 24 0.0 0.0 0.0 0.003981928172141818 0.0 25 0.0 0.0 0.0 0.004594532506317482 0.0 26 0.0 0.0 0.0 0.005513439007580979 0.0 27 0.0 0.0 0.0 0.007657554177195804 0.0 28 0.0 0.0 0.0 0.025116777701202235 0.0 29 0.0 0.0 0.0 0.06325139750363734 0.0 30 0.0 0.0 0.0 0.11287234857186615 0.0 31 0.0 0.0 0.0 0.1847002067539628 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGAAC 25 1.2330417E-4 37.0 24 CCTTTAT 30 8.306488E-6 37.0 1 GATTACG 70 0.0 37.0 1 CGTCTTC 335 0.0 33.686565 37 CTTATAC 1860 0.0 32.82258 1 TTATACA 1885 0.0 32.387268 2 GTACTAG 35 2.38674E-5 31.714285 1 TATACAC 1940 0.0 31.659794 3 TAGAACC 30 3.5977917E-4 30.833332 25 CTTTATA 30 3.5977917E-4 30.833332 2 CGGCGTA 25 0.0054946262 29.6 12 TTACTCG 25 0.0054946262 29.6 29 TCGGCGT 25 0.0054946262 29.6 11 GCAGCAT 25 0.0054946262 29.6 25 TATCCGG 25 0.0054946262 29.6 10 TCAGAGT 140 0.0 29.071428 3 GATACAC 45 4.0047707E-6 28.777779 3 TGCTTTG 35 8.865276E-4 26.42857 7 CGCGGAT 35 8.865276E-4 26.42857 18 CGACGAG 35 8.865276E-4 26.42857 24 >>END_MODULE