FastQCFastQC Report
Fri 10 Feb 2017
ERR1631064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778599
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT273933.518242381508325No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG210122.698693422416417No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT182922.3493479955663954RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC109011.4000788595926787No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC74950.9626264611179824RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC51800.6652975408393794RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41070.5274859073797937No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38900.4996153347230089No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG24890.31967675273150875No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA20570.26419247905532883No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19070.24492710625109976No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT17130.22001055742429673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15420.1980480324274755No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT13530.1737736626941468No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10750.13806850509697546No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10610.13627040363524742No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8740.11225290553930842No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8340.10711547279151398No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8210.1054458071484808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC32050.034.6333851
TTATACA34100.032.605572
CGTCTTC10550.031.5639837
GATTACG1000.031.4499991
TATACAC36250.030.977933
GGTATCA6400.030.06251
GACCGTT502.7188798E-729.5999987
GTTACGG502.7188798E-729.59999817
CGCCGTT502.7188798E-729.59999825
TTCTGCG250.005495268429.59999818
CCGTTTA502.7188798E-729.59999827
GGACCGT754.620233E-1027.1333336
TAGGACC1051.8189894E-1224.6666684
TACCGGG601.3364806E-624.66666832
CCGTCTT30100.024.03155937
TTACGGG1350.023.2962953
GCCGTCT31200.023.24359136
GTCGCAA400.001930630923.12518
TTAGCGA400.001930630923.12527
TAGCGAG400.001930630923.12528
Produced by FastQC (version 0.11.5)