##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631064.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	778599
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.448808693563695	31.0	31.0	34.0	28.0	34.0
2	31.471879619675853	31.0	31.0	34.0	28.0	34.0
3	30.959939583790884	31.0	31.0	34.0	27.0	34.0
4	35.08403555617205	37.0	35.0	37.0	32.0	37.0
5	35.281670025263324	37.0	35.0	37.0	33.0	37.0
6	35.47203631137466	37.0	35.0	37.0	33.0	37.0
7	35.46121045621687	37.0	35.0	37.0	33.0	37.0
8	35.48189376045949	37.0	35.0	37.0	33.0	37.0
9	37.284915598401746	39.0	37.0	39.0	34.0	39.0
10	37.107454543352866	39.0	37.0	39.0	33.0	39.0
11	37.158640070177334	39.0	37.0	39.0	33.0	39.0
12	37.07914728891252	39.0	37.0	39.0	33.0	39.0
13	37.163945753847614	39.0	37.0	39.0	33.0	39.0
14	38.22504267280076	40.0	38.0	41.0	33.0	41.0
15	38.21122040999282	40.0	38.0	41.0	33.0	41.0
16	38.211198575903644	40.0	38.0	41.0	33.0	41.0
17	38.181405319041	40.0	38.0	41.0	33.0	41.0
18	38.28034071453983	40.0	38.0	41.0	33.0	41.0
19	38.29266541570179	40.0	38.0	41.0	34.0	41.0
20	38.300762009712315	40.0	38.0	41.0	34.0	41.0
21	38.26879690315554	40.0	38.0	41.0	34.0	41.0
22	38.16339476418541	40.0	38.0	41.0	33.0	41.0
23	38.1795352935208	40.0	38.0	41.0	33.0	41.0
24	38.10875302947987	40.0	37.0	41.0	33.0	41.0
25	38.05997567425594	40.0	37.0	41.0	33.0	41.0
26	37.97379523991169	40.0	37.0	41.0	33.0	41.0
27	37.87857934572225	40.0	37.0	41.0	33.0	41.0
28	37.92504357185149	40.0	37.0	41.0	33.0	41.0
29	37.86124307891482	40.0	37.0	41.0	33.0	41.0
30	37.76656533080572	40.0	37.0	41.0	33.0	41.0
31	37.73063284180945	40.0	37.0	41.0	33.0	41.0
32	37.588900062805116	40.0	37.0	41.0	32.0	41.0
33	37.52794699196891	39.0	37.0	41.0	32.0	41.0
34	37.469357140196685	39.0	36.0	41.0	32.0	41.0
35	37.42649425442365	39.0	36.0	41.0	32.0	41.0
36	37.39250756808062	39.0	36.0	41.0	32.0	41.0
37	37.336026632451365	39.0	36.0	41.0	32.0	41.0
38	37.30015964572264	39.0	36.0	41.0	31.0	41.0
39	37.16879035292879	39.0	36.0	41.0	31.0	41.0
40	37.02585284594509	39.0	36.0	41.0	31.0	41.0
41	36.99303107247761	39.0	36.0	41.0	31.0	41.0
42	36.90528115242891	39.0	36.0	41.0	31.0	41.0
43	36.220237888823384	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	23.0
11	25.0
12	19.0
13	23.0
14	19.0
15	21.0
16	19.0
17	18.0
18	24.0
19	48.0
20	75.0
21	183.0
22	305.0
23	610.0
24	1082.0
25	1889.0
26	3055.0
27	4784.0
28	7126.0
29	9818.0
30	13577.0
31	18065.0
32	23403.0
33	29981.0
34	39465.0
35	53005.0
36	72532.0
37	107185.0
38	181909.0
39	210295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.49041290831352	23.130777203669666	15.993213451340162	25.385596436676643
2	12.668909156061078	21.39252683345342	42.95921263705707	22.97935137342843
3	13.266521020448266	29.8502823661474	35.56952937262955	21.313667240774777
4	10.111880441665093	20.40819471897601	36.78954121441204	32.69038362494686
5	11.74815277183762	38.28170855600894	35.645049634022136	14.325089038131312
6	25.973318743024333	41.08392124829341	17.19087746066974	15.751882548012519
7	22.848603709997057	33.81265580870255	21.790549435588794	21.548191045711594
8	24.518012481392862	34.37931464078428	23.161987107612518	17.94068577021034
9	22.255872406720275	13.9054892184552	18.98281400310044	44.85582437172408
10	13.951597677366653	28.71478129306613	33.11447869827729	24.21914233128992
11	29.49644168564306	22.768202887494077	25.745858908115732	21.989496518747135
12	19.20706294254167	27.57825273343531	27.12795675309113	26.086727570931888
13	29.83576911863488	19.65530394978673	26.901395968913395	23.607530962664992
14	21.470358939582507	23.469719329205407	25.70732816250727	29.352593568704815
15	24.397025940182303	28.548842215312376	22.055769401193682	24.99836244331164
16	25.688576532977827	26.455723678042226	22.6176761079837	25.238023680996253
17	19.247134917974464	32.44738305597618	23.813413580032854	24.4920684460165
18	23.891117250343243	23.834605490117504	25.224409484214593	27.049867775324653
19	22.379556100123427	26.630524827285935	26.253308827779126	24.736610244811512
20	20.664295741453557	24.482821067070468	27.847325773601046	27.005557417874925
21	23.795432565415574	24.399466220737505	25.932989895954144	25.87211131789278
22	19.037270790227062	31.63952175638551	28.685883233859794	20.63732421952764
23	22.065915830870576	23.710408053439576	26.900625354001225	27.323050761688624
24	22.329594566651124	27.942239843616544	28.40191163872545	21.326253951006873
25	19.482044030367366	30.600219111506693	26.819967659860854	23.097769198265087
26	22.62653817947364	25.40601773184913	25.987446683080762	25.97999740559646
27	18.63693634335518	30.70168340827563	30.558734342068256	20.102645906300936
28	18.341533960357	24.127696028379177	28.69050692333281	28.84026308793101
29	19.435550263999826	24.626926055646102	30.554752831688713	25.38277084866536
30	18.984740540380862	25.441979761083687	32.17638347853003	23.39689622000542
31	21.110995518874287	23.681638430051926	28.06785007430012	27.139515976773666
32	20.53457556457175	27.884829032659948	31.546277352013043	20.034318050755267
33	18.54099478679012	24.801213461615028	30.167261966686315	26.490529784908535
34	19.368249895003718	28.758321035603696	30.692179157692212	21.181249911700377
35	19.76434595985867	28.118196915228506	32.697447594975074	19.420009529937747
36	22.312384166946014	25.08980874622238	24.869284445523306	27.728522641308302
37	20.828565153564288	27.280666941519314	28.863895278570865	23.026872626345526
38	20.69704687522075	24.073367677071253	30.187297954402716	25.04228749330528
39	24.204243776321317	24.70668469905561	27.358755919285795	23.730315605337278
40	19.27924387264818	23.775653449336566	35.98501924610743	20.960083431907826
41	21.819575930613833	25.274499453505587	26.459063009328293	26.44686160655228
42	22.21374545818836	24.17264856492238	32.13721055382809	21.476395423061163
43	22.340383175421493	23.13874022442875	31.99027997724117	22.530596622908583
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	19.0
2	27.0
3	107.5
4	188.0
5	188.0
6	271.5
7	355.0
8	373.0
9	391.0
10	576.0
11	761.0
12	761.0
13	1234.0
14	1707.0
15	3935.5
16	6164.0
17	6824.0
18	7484.0
19	7484.0
20	7064.5
21	6645.0
22	7274.0
23	7903.0
24	10106.0
25	12309.0
26	12309.0
27	15067.5
28	17826.0
29	20805.0
30	23784.0
31	27508.0
32	31232.0
33	31232.0
34	35113.0
35	38994.0
36	41578.0
37	44162.0
38	46417.5
39	48673.0
40	48673.0
41	49596.5
42	50520.0
43	51574.5
44	52629.0
45	54197.0
46	55765.0
47	55765.0
48	57673.0
49	59581.0
50	79535.0
51	99489.0
52	73089.0
53	46689.0
54	46689.0
55	59749.5
56	72810.0
57	59007.0
58	45204.0
59	32085.0
60	18966.0
61	18966.0
62	15762.0
63	12558.0
64	9977.5
65	7397.0
66	5807.0
67	4217.0
68	4217.0
69	3235.0
70	2253.0
71	1666.0
72	1079.0
73	752.5
74	426.0
75	426.0
76	302.5
77	179.0
78	133.5
79	88.0
80	76.5
81	65.0
82	65.0
83	51.0
84	37.0
85	26.5
86	16.0
87	14.0
88	12.0
89	12.0
90	7.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	778599.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.392309193735954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.35526324270072	32.01912973904705
2	13.194014853672615	10.922614846599235
3	3.8364860468630857	4.764030500076318
4	1.5490411957002026	2.564735685050289
5	0.8172110549523078	1.6913126331562536
6	0.5002685831177506	1.2424363123393278
7	0.3623856271235859	1.0499984547685761
8	0.24954780823096429	0.8263488029532159
9	0.21042611272923759	0.7839020448472086
>10	1.566660818181031	13.304958760120572
>50	0.22894077275459312	6.577955289301704
>100	0.12133235785064048	8.845573893538926
>500	0.003430992124310988	1.0159357352987075
>1k	0.003119083749373625	2.736274237377151
>5k	6.23816749874725E-4	1.636419550757544
>10k+	0.00124763349974945	10.018373514767962
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	27393	3.518242381508325	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	21012	2.698693422416417	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	18292	2.3493479955663954	RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	10901	1.4000788595926787	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	7495	0.9626264611179824	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	5180	0.6652975408393794	RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4107	0.5274859073797937	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3890	0.4996153347230089	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	2489	0.31967675273150875	No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	2057	0.26419247905532883	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1907	0.24492710625109976	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	1713	0.22001055742429673	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1542	0.1980480324274755	No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	1353	0.1737736626941468	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1075	0.13806850509697546	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1061	0.13627040363524742	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	874	0.11225290553930842	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	834	0.10711547279151398	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	821	0.1054458071484808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.284358186948609E-4	0.0
5	0.0	0.0	0.0	1.284358186948609E-4	0.0
6	0.0	0.0	0.0	1.284358186948609E-4	0.0
7	0.0	0.0	0.0	1.284358186948609E-4	0.0
8	0.0	0.0	0.0	1.284358186948609E-4	0.0
9	0.0	0.0	0.0	3.853074560845827E-4	0.0
10	1.284358186948609E-4	0.0	0.0	6.421790934743045E-4	0.0
11	1.284358186948609E-4	0.0	0.0	7.706149121691654E-4	0.0
12	1.284358186948609E-4	0.0	0.0	8.990507308640263E-4	0.0
13	1.284358186948609E-4	0.0	0.0	8.990507308640263E-4	0.0
14	1.284358186948609E-4	0.0	0.0	8.990507308640263E-4	0.0
15	1.284358186948609E-4	0.0	0.0	8.990507308640263E-4	0.0
16	1.284358186948609E-4	0.0	0.0	0.001027486549558887	0.0
17	1.284358186948609E-4	0.0	0.0	0.001155922368253748	0.0
18	1.284358186948609E-4	0.0	0.0	0.001155922368253748	0.0
19	1.284358186948609E-4	0.0	0.0	0.001155922368253748	0.0
20	1.284358186948609E-4	0.0	0.0	0.0014127940056434698	0.0
21	1.284358186948609E-4	0.0	0.0	0.002054973099117774	0.0
22	1.284358186948609E-4	0.0	0.0	0.002568716373897218	0.0
23	1.284358186948609E-4	0.0	0.0	0.003467767104761244	0.0
24	1.284358186948609E-4	0.0	0.0	0.003981510379540688	0.0
25	1.284358186948609E-4	0.0	0.0	0.00423838201693041	0.0
26	1.284358186948609E-4	0.0	0.0	0.004752125291709854	0.0
27	1.284358186948609E-4	0.0	0.0	0.005651176022573879	0.0
28	1.284358186948609E-4	0.0	0.0	0.010660172951673454	0.0
29	1.284358186948609E-4	0.0	0.0	0.025173420464192735	0.0
30	1.284358186948609E-4	0.0	0.0	0.04546627981798076	0.0
31	1.284358186948609E-4	0.0	0.0	0.0942718909220279	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3205	0.0	34.633385	1
TTATACA	3410	0.0	32.60557	2
CGTCTTC	1055	0.0	31.56398	37
GATTACG	100	0.0	31.449999	1
TATACAC	3625	0.0	30.97793	3
GGTATCA	640	0.0	30.0625	1
GACCGTT	50	2.7188798E-7	29.599998	7
GTTACGG	50	2.7188798E-7	29.599998	17
CGCCGTT	50	2.7188798E-7	29.599998	25
TTCTGCG	25	0.0054952684	29.599998	18
CCGTTTA	50	2.7188798E-7	29.599998	27
GGACCGT	75	4.620233E-10	27.133333	6
TAGGACC	105	1.8189894E-12	24.666668	4
TACCGGG	60	1.3364806E-6	24.666668	32
CCGTCTT	3010	0.0	24.031559	37
TTACGGG	135	0.0	23.296295	3
GCCGTCT	3120	0.0	23.243591	36
GTCGCAA	40	0.0019306309	23.125	18
TTAGCGA	40	0.0019306309	23.125	27
TAGCGAG	40	0.0019306309	23.125	28
>>END_MODULE