FastQCFastQC Report
Fri 10 Feb 2017
ERR1631063.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631063.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910781
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT162881.788355268719923No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG141291.5513059670766078No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT93501.0265914638096314RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC79230.8699127452153701No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC38110.4184320928960969No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC34630.3802231271842518RNA PCR Primer, Index 47 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25240.27712479728935935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.2722937786361376No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG18930.2078435979670195No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA13600.1493223947359464No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC12770.1402093368219144No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT11680.1282415860673422No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA11160.1225322003862619No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC10400.11418771362160607No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC9610.1055138392215033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9360.10276894225944547No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT9270.10178077935310463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG600.037.01
CTTATAC18150.033.6363681
TTATACA20350.030.3636362
CGTCTTC4550.030.08791437
TTACGGG950.029.2105273
TATACAC22150.028.4808123
GGTATCA4550.028.461541
CCGTCTT15600.025.85256637
GCCGTCT15600.025.85256636
ATTACGG901.4188117E-1024.6666682
GTATCAA13750.023.8145471
TCGCTCG502.7015054E-422.237
TGCCGTC20750.019.43614435
GACGTCG500.007033787718.525
ATGCCGT21800.018.534
TCGGTGT500.007033787718.533
TACCGTT500.007033787718.59
ATCTGCG902.151217E-618.536
GTTCTAT1054.794565E-717.6190471
CGTTCGC650.001579593617.07692334
Produced by FastQC (version 0.11.5)