##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631063.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	910781
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278945212954596	31.0	31.0	34.0	28.0	34.0
2	31.27714126667113	31.0	31.0	34.0	28.0	34.0
3	30.71138616198625	31.0	31.0	34.0	26.0	34.0
4	34.918654429550024	37.0	35.0	37.0	32.0	37.0
5	35.10300610135697	37.0	35.0	37.0	32.0	37.0
6	35.3064501784732	37.0	35.0	37.0	33.0	37.0
7	35.315121856955734	37.0	35.0	37.0	33.0	37.0
8	35.337515824330985	37.0	35.0	37.0	33.0	37.0
9	37.08884902078546	39.0	37.0	39.0	33.0	39.0
10	36.89892630610432	39.0	37.0	39.0	33.0	39.0
11	36.95864538236964	39.0	37.0	39.0	33.0	39.0
12	36.886467767772935	39.0	37.0	39.0	33.0	39.0
13	36.970425382172	39.0	37.0	39.0	33.0	39.0
14	38.05279974000336	40.0	37.0	41.0	33.0	41.0
15	38.07236646350769	40.0	37.0	41.0	33.0	41.0
16	38.058369684918766	40.0	37.0	41.0	33.0	41.0
17	38.03341857153366	40.0	37.0	41.0	33.0	41.0
18	38.07679782516324	40.0	37.0	41.0	33.0	41.0
19	38.11082686178126	40.0	37.0	41.0	33.0	41.0
20	38.12795062698937	40.0	37.0	41.0	33.0	41.0
21	38.078856497884786	40.0	37.0	41.0	33.0	41.0
22	37.97870399140957	40.0	37.0	41.0	33.0	41.0
23	38.00031731008881	40.0	37.0	41.0	33.0	41.0
24	37.936476496545275	40.0	37.0	41.0	33.0	41.0
25	37.90374854108727	40.0	37.0	41.0	33.0	41.0
26	37.80312720621093	40.0	37.0	41.0	33.0	41.0
27	37.72828813951982	40.0	37.0	41.0	32.0	41.0
28	37.72072869328631	40.0	37.0	41.0	32.0	41.0
29	37.631518444060646	39.0	37.0	41.0	32.0	41.0
30	37.596405722122	39.0	37.0	41.0	32.0	41.0
31	37.57364942834776	39.0	37.0	41.0	32.0	41.0
32	37.43812837553704	39.0	36.0	41.0	32.0	41.0
33	37.4459370584147	39.0	37.0	41.0	32.0	41.0
34	37.36202665624338	39.0	36.0	40.0	32.0	41.0
35	37.345271805186975	39.0	36.0	40.0	31.0	41.0
36	37.350170897284855	39.0	36.0	40.0	32.0	41.0
37	37.26439286722055	39.0	36.0	40.0	31.0	41.0
38	37.22710069709403	39.0	36.0	40.0	31.0	41.0
39	37.171859096753224	39.0	36.0	40.0	31.0	41.0
40	37.10721238146162	39.0	36.0	40.0	31.0	41.0
41	37.06290645061765	39.0	36.0	40.0	31.0	41.0
42	37.02518388064749	39.0	36.0	40.0	31.0	41.0
43	36.30188815972226	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	7.0
10	14.0
11	10.0
12	17.0
13	11.0
14	9.0
15	11.0
16	4.0
17	9.0
18	25.0
19	44.0
20	88.0
21	172.0
22	397.0
23	667.0
24	1387.0
25	2295.0
26	3575.0
27	5561.0
28	8389.0
29	11923.0
30	16363.0
31	21769.0
32	28318.0
33	36916.0
34	48936.0
35	66101.0
36	91804.0
37	136408.0
38	231579.0
39	197970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.84123845359093	23.815055430449252	15.549951085936137	23.793755030023682
2	13.140919716155693	23.1927323912115	41.84650316596416	21.81984472666865
3	14.49086004209574	29.32011098167397	35.63161726035128	20.55741171587901
4	11.675803513687704	20.09802576030901	38.231254275177015	29.99491645082627
5	11.471034200318188	39.63982560022662	36.60759282418057	12.281547375274627
6	26.10440929268397	41.24537073127349	18.3853198518634	14.264900124179139
7	22.40692328891358	34.77575838758165	23.654643651986593	19.162674671518182
8	23.385204566190996	36.810495607615884	23.069541415554344	16.73475841063878
9	23.185046679717736	15.910081567358125	20.30125793137977	40.603613821544364
10	13.546505691269362	29.83746916108263	34.86491264090928	21.751112506738725
11	29.9518764664612	24.051116569186227	26.340799819056393	19.656207145296182
12	19.23524974719499	27.284824782247323	30.505137898133576	22.97478757242411
13	27.91307679892312	21.72915333104226	28.234998314633263	22.122771555401354
14	20.42236278534576	23.533209410385155	27.616518131142392	28.42790967312669
15	22.79516151522704	29.906969952161937	23.969538231473866	23.328330301137157
16	23.477433104116137	29.09480983902826	24.209003042443793	23.218754014411807
17	19.895452364509143	31.785357841237357	26.067847265149364	22.251342529104143
18	22.347194331019203	27.667134031122742	27.012640799489667	22.973030838368388
19	22.39418696700963	29.535091311742338	27.606416910322018	20.464304810926006
20	21.941498560027053	26.985191829869095	28.1596783419944	22.91363126810946
21	23.873576633680322	27.305027223888068	26.286780246843094	22.534615895588512
22	20.15500982124133	31.53370568775589	26.992767745484368	21.318516745518405
23	22.968968390864543	27.012311411854224	26.963781633565038	23.054938563716195
24	22.39868859802741	29.671896976331304	26.89691594356931	21.03249848207198
25	21.64900233975017	29.46064970613133	27.574576105562148	21.31577184855635
26	23.984580266825944	28.67846386782333	26.444337332465214	20.892618532885514
27	20.325962004038292	30.19858780541096	28.35423663866506	21.12121355188569
28	22.503433866099535	27.452483088689817	27.87497762908976	22.16910541612089
29	20.419727684262188	29.55320763169192	26.828403315396347	23.198661368649542
30	21.741121081796834	28.36148316664489	29.674092893900948	20.22330285765733
31	23.970526394380208	26.5244883237573	26.763294359456335	22.741690922406153
32	20.88734833071836	29.27531426325319	27.763205424794762	22.074131981233688
33	23.724583626579825	26.759890687223383	29.032994759442722	20.482530926754073
34	20.352093423117083	29.194504496690204	27.122656269728946	23.330745810463764
35	20.555764777701775	28.89772623715251	30.62953662845404	19.916972356691677
36	23.350509068590583	27.26670846229774	26.542165460192958	22.84061700891872
37	21.771644336014916	27.946344950103263	28.72875038016823	21.55326033371359
38	21.43742568191475	26.525366690785162	29.415962783589027	22.621244843711057
39	22.782095805687646	27.08148281529808	27.593460996661108	22.542960382353165
40	20.384263615512403	26.072019508531692	32.15657770638606	21.387139169569853
41	21.34816163270863	27.52428959321725	27.70940544433843	23.41814332973569
42	22.176571535857686	25.92599099015021	30.339675509260733	21.557761964731366
43	21.678208043426466	25.88371957693452	30.2554620704648	22.182610309174216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	19.0
2	25.0
3	101.0
4	177.0
5	177.0
6	213.5
7	250.0
8	257.5
9	265.0
10	426.0
11	587.0
12	587.0
13	916.0
14	1245.0
15	3123.5
16	5002.0
17	5683.0
18	6364.0
19	6364.0
20	7280.0
21	8196.0
22	10848.5
23	13501.0
24	17394.0
25	21287.0
26	21287.0
27	26663.5
28	32040.0
29	38407.5
30	44775.0
31	48035.5
32	51296.0
33	51296.0
34	54370.0
35	57444.0
36	59064.5
37	60685.0
38	62999.5
39	65314.0
40	65314.0
41	67120.0
42	68926.0
43	64018.0
44	59110.0
45	58672.5
46	58235.0
47	58235.0
48	56453.5
49	54672.0
50	55922.0
51	57172.0
52	71449.0
53	85726.0
54	85726.0
55	63307.0
56	40888.0
57	45024.5
58	49161.0
59	39577.0
60	29993.0
61	29993.0
62	22501.0
63	15009.0
64	12196.5
65	9384.0
66	7656.0
67	5928.0
68	5928.0
69	4653.0
70	3378.0
71	2826.5
72	2275.0
73	1765.5
74	1256.0
75	1256.0
76	949.0
77	642.0
78	488.5
79	335.0
80	229.5
81	124.0
82	124.0
83	90.5
84	57.0
85	39.5
86	22.0
87	18.5
88	15.0
89	15.0
90	10.5
91	6.0
92	3.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	910781.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.15225637605919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.66296566977223	31.640816329205112
2	14.662073134076255	12.947272240405292
3	5.743085342518886	7.607105292974446
4	2.749131831546946	4.855214937521837
5	1.4884328797450164	3.2858835052529027
6	0.8801831827545398	2.3317244125724526
7	0.5412534856698166	1.6728293864610593
8	0.37927021300170943	1.3396508544203243
9	0.27891083293664165	1.1083088341690934
>10	1.365608245720506	10.959005483430676
>50	0.13264667811613895	4.13564310630422
>100	0.10771184965812407	8.920059662145254
>500	0.0052359926899290905	1.7302238446341673
>1k	0.0024933298523471857	2.216254147749256
>5k	4.986659704694371E-4	1.9015178767173107
>10k+	4.986659704694371E-4	3.3484900860366142
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	16288	1.788355268719923	No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG	14129	1.5513059670766078	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	9350	1.0265914638096314	RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	7923	0.8699127452153701	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	3811	0.4184320928960969	No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC	3463	0.3802231271842518	RNA PCR Primer, Index 47 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2524	0.27712479728935935	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2480	0.2722937786361376	No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG	1893	0.2078435979670195	No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA	1360	0.1493223947359464	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1277	0.1402093368219144	No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT	1168	0.1282415860673422	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1116	0.1225322003862619	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1040	0.11418771362160607	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	961	0.1055138392215033	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	936	0.10276894225944547	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	927	0.10178077935310463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0979587848231353E-4	0.0	0.0	0.0	0.0
2	1.0979587848231353E-4	0.0	0.0	0.0	0.0
3	1.0979587848231353E-4	0.0	0.0	0.0	0.0
4	1.0979587848231353E-4	0.0	0.0	0.0	0.0
5	1.0979587848231353E-4	0.0	0.0	0.0	0.0
6	1.0979587848231353E-4	0.0	0.0	0.0	0.0
7	1.0979587848231353E-4	0.0	0.0	0.0	0.0
8	1.0979587848231353E-4	0.0	0.0	0.0	0.0
9	1.0979587848231353E-4	0.0	0.0	0.0	0.0
10	1.0979587848231353E-4	0.0	0.0	1.0979587848231353E-4	0.0
11	1.0979587848231353E-4	0.0	0.0	3.293876354469406E-4	0.0
12	1.0979587848231353E-4	0.0	0.0	3.293876354469406E-4	0.0
13	1.0979587848231353E-4	0.0	0.0	3.293876354469406E-4	0.0
14	1.0979587848231353E-4	0.0	0.0	3.293876354469406E-4	0.0
15	1.0979587848231353E-4	0.0	0.0	3.293876354469406E-4	0.0
16	1.0979587848231353E-4	0.0	0.0	4.3918351392925413E-4	0.0
17	1.0979587848231353E-4	0.0	0.0	5.489793924115677E-4	0.0
18	1.0979587848231353E-4	0.0	0.0	5.489793924115677E-4	0.0
19	1.0979587848231353E-4	0.0	0.0	5.489793924115677E-4	0.0
20	1.0979587848231353E-4	0.0	0.0	5.489793924115677E-4	0.0
21	1.0979587848231353E-4	0.0	0.0	6.587752708938812E-4	0.0
22	1.0979587848231353E-4	0.0	0.0	6.587752708938812E-4	0.0
23	1.0979587848231353E-4	0.0	0.0	0.0010979587848231353	0.0
24	1.0979587848231353E-4	0.0	0.0	0.0015371422987523894	0.0
25	1.0979587848231353E-4	0.0	0.0	0.0015371422987523894	0.0
26	1.0979587848231353E-4	0.0	0.0	0.00186652993419933	0.0
27	1.0979587848231353E-4	0.0	0.0	0.0024155093266108978	0.0
28	1.0979587848231353E-4	0.0	0.0	0.0061485691950095575	0.0
29	1.0979587848231353E-4	0.0	0.0	0.016359585893864718	0.0
30	1.0979587848231353E-4	0.0	0.0	0.03458570172192876	0.0
31	1.0979587848231353E-4	0.0	0.0	0.09211874204666105	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	60	0.0	37.0	1
CTTATAC	1815	0.0	33.636368	1
TTATACA	2035	0.0	30.363636	2
CGTCTTC	455	0.0	30.087914	37
TTACGGG	95	0.0	29.210527	3
TATACAC	2215	0.0	28.480812	3
GGTATCA	455	0.0	28.46154	1
CCGTCTT	1560	0.0	25.852566	37
GCCGTCT	1560	0.0	25.852566	36
ATTACGG	90	1.4188117E-10	24.666668	2
GTATCAA	1375	0.0	23.814547	1
TCGCTCG	50	2.7015054E-4	22.2	37
TGCCGTC	2075	0.0	19.436144	35
GACGTCG	50	0.0070337877	18.5	25
ATGCCGT	2180	0.0	18.5	34
TCGGTGT	50	0.0070337877	18.5	33
TACCGTT	50	0.0070337877	18.5	9
ATCTGCG	90	2.151217E-6	18.5	36
GTTCTAT	105	4.794565E-7	17.619047	1
CGTTCGC	65	0.0015795936	17.076923	34
>>END_MODULE