FastQCFastQC Report
Fri 10 Feb 2017
ERR1631062.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631062.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271086
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG208567.693499479869857No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT173776.410142906679061No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT134774.971485063780498TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC107573.9681134400153457No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC49681.8326287598769395No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC46571.7179050190714387RNA PCR Primer, Index 1 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33901.2505256634425976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32801.2099481345403305No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG24960.9207410194550807No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22490.8296260227381718No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA17110.6311650177434468No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT15800.5828408696871104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13790.5086946577838768No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13580.5009480386298075No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12840.47365042827737325No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT11900.438975085397254No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11610.42827737323211085No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10610.39138871059368613No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9940.36667330662594155No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9150.33753126314158605No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8680.32019359170152645No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8590.3168736120640682No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7780.2869937953269442No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6590.2430962867872188No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6350.23424300775399687No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6280.23166080136930714No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6190.22834082173184894No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5730.21137203691817358No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5730.21137203691817358No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5660.20878983053348385No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5490.20251875788495163No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5350.19735434511557215No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT5010.1848121998185078No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4980.18370553993935504No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4960.18296776668658654No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4940.18222999343381804No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4370.16120345572991598No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4370.16120345572991598No Hit
CTGATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT4300.15862124934522623No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4270.1575145894660735No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4170.15382572320223104No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4120.1519812900703098No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4060.1497679703120043No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4030.14866131043285155No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA3930.1449724441690091No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3910.1442346709162406No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.1442346709162406No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3900.14386578428985636No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3890.1434968976634721No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3740.1379635982677084No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3670.13538139188301868No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3650.13464361863025018No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3580.13206141224556045No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3390.12505256634425974No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3310.12210147333318577No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3300.12173258670680152No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3250.1198881535748803No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3240.11951926694849604No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3200.11804372044295905No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3130.11546151405826934No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3110.11472374080550084No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2990.11029710128888988No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2950.10882155478335288No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2940.10845266815696865No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2900.10697712165143164No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2880.10623934839866316No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA2850.10513268851951041No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2850.10513268851951041No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2840.10476380189312617No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2830.10439491526674193No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2810.10365714201397343No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.10218159550843643No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2750.10144382225566793No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2740.1010749356292837No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2730.10070604900289945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACA700.037.02
ACAAATC251.231138E-437.01
TTTACAC650.037.02
TTACTTT308.288553E-637.03
TTATCAA200.001839456137.02
TACGCCT200.001839456137.032
CTACGCC200.001839456137.031
GTGTCAA200.001839456137.01
CAAATCT251.231138E-437.02
TTACGGG200.001839456137.03
CGACTAC200.001839456137.028
TCGACTA200.001839456137.027
CTTATAC18450.035.897021
CGTCTTC5500.034.9818237
GATACAC800.032.3753
ATTACTT352.381629E-531.7142852
TTATACA20900.031.6004772
AAATCTC303.5922887E-430.8333323
CGTTACT303.5922887E-430.8333321
AATCTCC303.5922887E-430.8333324
Produced by FastQC (version 0.11.5)