FastQCFastQC Report
Fri 10 Feb 2017
ERR1631061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080152
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT104570.9681044889978448No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG83670.7746132025863026No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT53170.49224553581347813Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC45030.41688577163214063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26560.24589131899954822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24380.225708974292507No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC20810.19265807034565507No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC15700.1453499137158474Illumina PCR Primer Index 11 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14670.1358142187395848No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG11670.10804034987668402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC14000.029.4678551
GATTACG950.027.2631591
TTATACA15750.025.958732
TATACAC16650.025.6666663
CGTCTTC3650.024.83561737
GTATCAA13400.024.7126871
GGTATCA4000.024.0500011
GCCGTCT8800.023.12536
CCGTCTT9000.022.81666837
TGCCGTC10450.019.47368635
TTACGGG1355.638867E-1119.1851863
ATGCCGT11350.017.7665234
TAATACG650.001579851117.0769212
TATGCCG12250.016.76326633
CTTAGCG904.445557E-516.44444524
TTAGCGT803.3823293E-416.187525
AATACGA700.002592358515.85714216
TACACAT27950.015.4221835
ATACACA28200.015.4166674
ATCAACG23250.015.3569892
Produced by FastQC (version 0.11.5)