##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631061.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1080152
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5398342085188	31.0	31.0	34.0	30.0	34.0
2	31.522854190891653	31.0	31.0	34.0	30.0	34.0
3	31.004181818855123	31.0	31.0	34.0	27.0	34.0
4	35.14720798554278	37.0	35.0	37.0	32.0	37.0
5	35.34170746339404	37.0	35.0	37.0	33.0	37.0
6	35.52483076455906	37.0	35.0	37.0	33.0	37.0
7	35.539436116398434	37.0	35.0	37.0	33.0	37.0
8	35.556367066857256	37.0	35.0	37.0	33.0	37.0
9	37.321751938616046	39.0	37.0	39.0	34.0	39.0
10	37.15768521467349	39.0	37.0	39.0	33.0	39.0
11	37.219378383783024	39.0	37.0	39.0	33.0	39.0
12	37.15734915086025	39.0	37.0	39.0	33.0	39.0
13	37.228818721809525	39.0	37.0	39.0	34.0	39.0
14	38.40588639376681	40.0	38.0	41.0	34.0	41.0
15	38.41608403261763	40.0	38.0	41.0	34.0	41.0
16	38.41437686547819	40.0	38.0	41.0	34.0	41.0
17	38.384255178900744	40.0	38.0	41.0	34.0	41.0
18	38.39846336441538	40.0	38.0	41.0	34.0	41.0
19	38.438188329049986	40.0	38.0	41.0	34.0	41.0
20	38.43374358423629	40.0	38.0	41.0	34.0	41.0
21	38.398159703449146	40.0	38.0	41.0	34.0	41.0
22	38.3490221746569	40.0	38.0	41.0	34.0	41.0
23	38.32697250016664	40.0	38.0	41.0	34.0	41.0
24	38.311314518697365	40.0	38.0	41.0	34.0	41.0
25	38.26642824343241	40.0	38.0	41.0	34.0	41.0
26	38.17988023907746	40.0	38.0	41.0	34.0	41.0
27	38.12515183048312	40.0	38.0	41.0	33.0	41.0
28	38.07597912145698	40.0	37.0	41.0	33.0	41.0
29	38.026923988475694	40.0	37.0	41.0	33.0	41.0
30	37.94726020041624	40.0	37.0	41.0	33.0	41.0
31	37.929033136077145	40.0	37.0	41.0	33.0	41.0
32	37.85624986113066	40.0	37.0	41.0	33.0	41.0
33	37.83998455772891	40.0	37.0	41.0	33.0	41.0
34	37.78552277827565	40.0	37.0	41.0	33.0	41.0
35	37.74859834541805	40.0	37.0	41.0	33.0	41.0
36	37.717761944615205	40.0	37.0	41.0	33.0	41.0
37	37.66814670527852	40.0	37.0	41.0	33.0	41.0
38	37.62931976240381	40.0	37.0	41.0	33.0	41.0
39	37.56396877476503	40.0	37.0	41.0	32.0	41.0
40	37.49902050822477	40.0	37.0	41.0	32.0	41.0
41	37.44361071404765	40.0	37.0	41.0	32.0	41.0
42	37.378991104955595	40.0	37.0	41.0	32.0	41.0
43	36.77983839311504	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	9.0
10	7.0
11	13.0
12	7.0
13	6.0
14	7.0
15	10.0
16	10.0
17	10.0
18	21.0
19	38.0
20	87.0
21	147.0
22	296.0
23	635.0
24	1114.0
25	2109.0
26	3482.0
27	5395.0
28	8239.0
29	11912.0
30	16745.0
31	22302.0
32	29366.0
33	38576.0
34	51103.0
35	69013.0
36	96837.0
37	144975.0
38	257870.0
39	319809.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.29058132559121	21.884975447899926	14.73079714706819	24.093646079440674
2	14.19031765899614	22.206596849332318	40.51022448692406	23.09286100474748
3	15.031588146853405	28.206493160221896	33.27115072693473	23.490767965989974
4	12.05098912004977	18.881787007754465	38.906746457905925	30.160477414289836
5	12.810697013012984	38.23387819492072	35.82912404920789	13.126300742858414
6	28.267040194343018	39.94604463075567	18.085139869203594	13.701775305697716
7	25.256908286981833	32.657811122879004	23.650097393700147	18.43518319643902
8	24.591816707278234	35.80940460231523	21.750549922603486	17.84822876780305
9	24.652734059650864	15.440604655641058	20.22761611328776	39.67904517142032
10	14.490460601841223	29.04193113561795	33.95827624260289	22.509332019937936
11	32.52320043845681	23.34282582451359	25.31310408164777	18.820869655381838
12	21.34366274376199	25.947644405602176	30.57912219761663	22.129570653019204
13	28.414704597130775	21.540394314874202	26.74808730623098	23.296813781764047
14	21.526877698694257	21.57511165095283	27.45669128048645	29.441319369866463
15	23.445959457557823	28.947592561046964	23.50391426391841	24.1025337174768
16	23.704534176671434	27.889685896059074	24.521733978180848	23.884045949088648
17	21.683614898643892	29.278286759641237	26.054944118975847	22.98315422273902
18	23.48123227101371	26.737440656500194	26.865478191958168	22.915848880527925
19	23.750638798983847	27.387441767454952	26.85575733785615	22.006162095705047
20	23.11582073634081	26.947040786852224	26.439797361852772	23.497341114954192
21	23.832016234752146	26.326665136017898	26.743365748524283	23.097952880705677
22	22.467115739266326	28.627174693931966	26.79511772417215	22.110591842629557
23	22.903813537354	26.142154067205357	27.19117309415712	23.762859301283523
24	23.274131788859346	27.364019138047237	27.127385775335323	22.234463297758094
25	22.938345714306877	27.70026811041409	26.54098682407661	22.820399351202422
26	23.334493663854715	26.613476621808786	27.27727208763211	22.77475762670439
27	22.727449470074582	28.597549233811538	26.597830675682683	22.0771706204312
28	21.964593871973573	26.364807915922945	27.221076292966174	24.449521919137307
29	23.472252053414707	26.485068768099307	27.739799583762288	22.302879594723706
30	22.60959568653301	27.308193661632806	27.152382257311935	22.929828394522254
31	23.11026596256823	25.87496944874425	26.996756012116812	24.018008576570704
32	24.01680504225331	26.523211548004355	27.118961035113575	22.341022374628754
33	22.04171264784956	26.236122323524835	27.850247002273754	23.871918026351846
34	22.365000481413727	27.083132744280437	27.823954406416874	22.727912367888965
35	23.050274405824364	26.516730978603007	28.47923255245558	21.953762063117043
36	23.272835674979078	26.44137121442167	26.172520163828793	24.113272946770454
37	22.511276190758338	26.852517053155484	27.588709737148104	23.047497018938078
38	22.530069842022236	26.108640265444123	27.777386886290078	23.583903006243563
39	23.309589761440982	26.252231167465318	27.094242291825594	23.343936779268105
40	22.21150356616476	26.117713062606	29.42345151423133	22.247331856997903
41	22.751334997296677	26.1744643346492	26.92269236181574	24.15150830623838
42	22.810030440160272	26.05790666498789	28.12298639450744	23.009076500344396
43	23.217010198564648	25.50094801472385	28.44266362511943	22.839378161592073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	10.5
2	13.0
3	72.0
4	131.0
5	131.0
6	178.0
7	225.0
8	234.0
9	243.0
10	390.5
11	538.0
12	538.0
13	818.5
14	1099.0
15	2643.5
16	4188.0
17	4932.0
18	5676.0
19	5676.0
20	6089.5
21	6503.0
22	8104.5
23	9706.0
24	12903.5
25	16101.0
26	16101.0
27	20518.0
28	24935.0
29	29760.5
30	34586.0
31	40334.0
32	46082.0
33	46082.0
34	52329.5
35	58577.0
36	63295.0
37	68013.0
38	71631.5
39	75250.0
40	75250.0
41	78263.5
42	81277.0
43	82775.0
44	84273.0
45	84579.0
46	84885.0
47	84885.0
48	82980.5
49	81076.0
50	78978.0
51	76880.0
52	82570.0
53	88260.0
54	88260.0
55	74720.0
56	61180.0
57	58976.0
58	56772.0
59	48995.0
60	41218.0
61	41218.0
62	33903.0
63	26588.0
64	22044.5
65	17501.0
66	14450.0
67	11399.0
68	11399.0
69	9296.5
70	7194.0
71	5824.5
72	4455.0
73	3455.0
74	2455.0
75	2455.0
76	1929.0
77	1403.0
78	1057.5
79	712.0
80	545.5
81	379.0
82	379.0
83	289.0
84	199.0
85	152.0
86	105.0
87	73.0
88	41.0
89	41.0
90	32.0
91	23.0
92	13.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1080152.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.132270843166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0365066376556	44.36498597082572
2	12.318092663615603	13.828850273305743
3	3.627443780515523	6.108499702687659
4	1.5927761602943018	3.576245712887109
5	0.8405169970853295	2.359006386433914
6	0.5350779758930474	1.8021085119024953
7	0.3424159182893986	1.3454408146502346
8	0.2372087237698073	1.065205146320686
9	0.17816437151454464	0.9000693680811183
>10	1.1458947965712762	12.361067036612118
>50	0.09832707324334836	3.7423142760672614
>100	0.043775518058469826	3.9992398180753432
>500	0.002147477626898218	0.8358252009116683
>1k	0.0011563341067913479	1.4726620635543728
>5k	3.3038117336895654E-4	1.2688520134541015
>10k+	1.6519058668447827E-4	0.9696277042304545
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	10457	0.9681044889978448	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	8367	0.7746132025863026	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	5317	0.49224553581347813	Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	4503	0.41688577163214063	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2656	0.24589131899954822	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2438	0.225708974292507	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	2081	0.19265807034565507	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	1570	0.1453499137158474	Illumina PCR Primer Index 11 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1467	0.1358142187395848	No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG	1167	0.10804034987668402	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.257956287633592E-5	0.0
5	0.0	0.0	0.0	9.257956287633592E-5	0.0
6	0.0	0.0	0.0	9.257956287633592E-5	0.0
7	0.0	0.0	0.0	9.257956287633592E-5	0.0
8	0.0	0.0	0.0	9.257956287633592E-5	0.0
9	0.0	0.0	0.0	9.257956287633592E-5	0.0
10	0.0	0.0	0.0	1.8515912575267185E-4	0.0
11	0.0	0.0	0.0	1.8515912575267185E-4	0.0
12	0.0	0.0	0.0	1.8515912575267185E-4	0.0
13	0.0	0.0	0.0	1.8515912575267185E-4	0.0
14	0.0	0.0	0.0	1.8515912575267185E-4	0.0
15	0.0	0.0	0.0	1.8515912575267185E-4	0.0
16	0.0	0.0	0.0	1.8515912575267185E-4	0.0
17	0.0	0.0	0.0	1.8515912575267185E-4	0.0
18	0.0	0.0	0.0	1.8515912575267185E-4	0.0
19	0.0	0.0	0.0	1.8515912575267185E-4	0.0
20	0.0	0.0	0.0	3.703182515053437E-4	9.257956287633592E-5
21	0.0	0.0	0.0	5.554773772580155E-4	9.257956287633592E-5
22	0.0	0.0	0.0	7.406365030106874E-4	9.257956287633592E-5
23	0.0	0.0	0.0	0.0015738525688977108	9.257956287633592E-5
24	0.0	0.0	0.0	0.0016664321317740466	9.257956287633592E-5
25	0.0	0.0	0.0	0.0018515912575267184	9.257956287633592E-5
26	0.0	0.0	0.0	0.0019441708204030544	9.257956287633592E-5
27	0.0	0.0	0.0	0.002592227760537406	9.257956287633592E-5
28	0.0	0.0	0.0	0.006943467215725194	9.257956287633592E-5
29	0.0	0.0	0.0	0.014812730060213747	9.257956287633592E-5
30	0.0	0.0	0.0	0.029162562306045817	9.257956287633592E-5
31	0.0	0.0	0.0	0.06758308089972523	9.257956287633592E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1400	0.0	29.467855	1
GATTACG	95	0.0	27.263159	1
TTATACA	1575	0.0	25.95873	2
TATACAC	1665	0.0	25.666666	3
CGTCTTC	365	0.0	24.835617	37
GTATCAA	1340	0.0	24.712687	1
GGTATCA	400	0.0	24.050001	1
GCCGTCT	880	0.0	23.125	36
CCGTCTT	900	0.0	22.816668	37
TGCCGTC	1045	0.0	19.473686	35
TTACGGG	135	5.638867E-11	19.185186	3
ATGCCGT	1135	0.0	17.76652	34
TAATACG	65	0.0015798511	17.076921	2
TATGCCG	1225	0.0	16.763266	33
CTTAGCG	90	4.445557E-5	16.444445	24
TTAGCGT	80	3.3823293E-4	16.1875	25
AATACGA	70	0.0025923585	15.857142	16
TACACAT	2795	0.0	15.422183	5
ATACACA	2820	0.0	15.416667	4
ATCAACG	2325	0.0	15.356989	2
>>END_MODULE