FastQCFastQC Report
Fri 10 Feb 2017
ERR1631060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237372
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT136945.76900392632661No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG130855.5124446017221915No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT105214.432283504372883RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC71182.9986687562138754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41241.7373573968286067No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC37411.5760072797128557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37091.562526329979947No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC34501.4534148930792175RNA PCR Primer, Index 46 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21950.9247088957417051No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG16440.6925837925281836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14180.597374585039516No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13020.5485061422577221No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12520.5274421583000523No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA12440.5240719208668251No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11540.4861567497430194No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT10600.4465564599026002No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9770.4115902465328682No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT9260.39010498289604506No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9110.3837857877087441No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8950.3770453128422897No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8840.3724112363716024No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8820.3715686770132956No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8620.36314308343022766No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6470.27256795241224746No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6020.2536103668503446No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5550.233810221930135No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5550.233810221930135No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5540.23338894225098158No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5330.22454206898876025No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5260.22159311123468647No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5250.22117183155553308No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5120.21569519572653892No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4770.20095040695617006No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4730.19926528823955647No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4540.19126097433564193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.19126097433564193No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4450.1874694572232614No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4370.1840992197900342No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4230.17820130428188666No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4200.17693746524442647No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4040.17019699037797212No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT3960.16682675294474497No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3940.16598419358643818No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3900.16429907486982456No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3720.15671604064506345No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3560.1499755657786091No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3450.14534148930792173No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3260.13733717540400722No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3240.1364946160457004No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3200.1348094973290868No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3180.13396693797078005No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3130.13186053957501306No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.13101798021670627No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3040.1280690224626325No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3030.1276477427834791No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2980.1255413443877121No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2980.1255413443877121No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2910.12259238663363833No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2890.12174982727533154No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2880.12132854759617814No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2860.12048598823787136No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2850.12006470855871797No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2820.11880086952125778No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2780.11711575080464419No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA2760.1162731914463374No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2760.1162731914463374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2740.1154306320880306No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2700.11374551337141703No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2650.11163911497565004No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2570.10826887754242286No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2570.10826887754242286No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2510.10574119946750249No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2510.10574119946750249No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2470.1040560807508889No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2440.10279224171342871No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2430.10237096203427531No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA2410.10152840267596852No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2380.10026456363850832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG650.037.01
TTACGGG650.037.03
CTTATAC14750.035.620341
CGTCTTC3700.034.537
ATTACGG700.034.3571432
ACTCAGA507.2795956E-933.31
TCAGAGT451.0738404E-732.888893
CTCAGAG451.0738404E-732.888892
TTATACA16050.032.7352032
TAGGACC352.3803885E-531.7142874
GATACAC352.3803885E-531.7142873
AGGACCG352.3803885E-531.7142875
CTTAGGA352.3803885E-531.7142872
GACCGTT352.3803885E-531.7142877
TTAGGAC352.3803885E-531.7142873
TGATACA303.5909537E-430.8333342
TATACAC17500.030.0228583
ATACTTC250.005487669729.5999986
TACTTCA250.005487669729.5999987
ATATTCT250.005487669729.5999984
Produced by FastQC (version 0.11.5)