FastQCFastQC Report
Fri 10 Feb 2017
ERR1631059.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631059.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379523
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT193595.1008766267130055No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG139653.679618890027745No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT130013.425615838829267RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC69011.8183351206646239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64031.6871177767882315No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC61571.6222995707770016RNA PCR Primer, Index 27 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59041.5556369442695173No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC38621.0175931366478448RNA PCR Primer, Index 27 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA36560.9633144763294977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23550.6205157526684812No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA21550.5678180242040667No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA19450.5124854093164314No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT18940.49904748855800574No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT16750.44134347588947176No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT15550.4097248388108231No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA15300.40313762275277126No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG15130.398658315833296No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG15030.39602342941007523No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC14830.39075365656363387No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG13570.3575540876310527No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC12840.33831941674154137No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT12420.3272528937640143No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG11010.29010099519660204No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG10110.2663870173876155No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT10010.2637521309643948No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9460.24926025563668078No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9340.24609839192881588No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9060.23872070994379788No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9050.23845722130147579No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8870.23371442573967852No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT8820.23239698252806815No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.23055256203181362No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8670.22844465289323704No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8390.22106697090821897No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8200.2160606867040996No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7880.20762905014979327No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7700.202886254587996No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7440.19603554988762212No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7200.18971182247189236No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6850.1804897199906198No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6800.17917227677900943No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6750.1778548335673991No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6100.16072807181646434No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6090.16046458317414228No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5910.15572178761234498No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5710.15045201476590353No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5710.15045201476590353No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5600.14755363970036073No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5530.1457092192041062No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG5500.14491875327714No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5470.14412828735017377No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG5450.14360131006552962No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5450.14360131006552962No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.1428108441385634No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5230.13780455993444402No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5200.13701409400747783No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5160.13596013943818952No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA5060.13332525301496878No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5040.13279827573032466No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4980.13121734387639222No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4960.13069036659174804No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4950.13042687794942598No Hit
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT4820.12700152559923905No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4820.12700152559923905No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4670.12304919596440794No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4630.12199524139511968No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4620.12173175275279759No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4440.11698895719100028No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4440.11698895719100028No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4410.11619849126403407No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4370.11514453669474578No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT4360.1148810480524237No Hit
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT4320.11382709348313541RNA PCR Primer, Index 27 (100% over 23bp)
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4310.11356360484081333No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4300.11330011619849127No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4230.11145569570223676No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4220.11119220705991467No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC4150.10934778656366018No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG4140.1090842979213381No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4050.10671290014043944No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA4020.10592243421347323No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3940.10381452507489664No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT3850.101443127293998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG308.297309E-637.01
TCGCCGA200.001840383337.01
GACAGAC355.618058E-737.016
TTACTTT308.297309E-637.03
TTGAAAG200.001840383337.02
ACTCAGA452.6102498E-937.01
CTTATAC21850.035.052631
ATTACGG900.034.9444472
TCAGAGT507.3050614E-933.33
TTACGGG950.033.1052633
GATTACG950.033.1052631
TTATACA23250.032.78282
CGTCTTC7300.032.6917837
AGACAGA401.5963305E-632.37515
ACAGACA401.5963305E-632.37517
ATTACTT352.384125E-531.7142852
TATACAC24350.031.6817263
TAGCAAC303.594976E-430.8333341
TTGTGTG250.005491764329.5999983
TGAAAGG250.005491764329.5999983
Produced by FastQC (version 0.11.5)