FastQCFastQC Report
Fri 10 Feb 2017
ERR1631058.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631058.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987566
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT165251.6733058853788No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG130181.3181903791746576No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT98560.9980092469769108Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC69750.7062819092597356No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC35620.3606847542341474Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC30280.30661241881555257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25420.25740051804132585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22420.2270228015140254No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG17840.1806461542823467No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA11540.11685294957501574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20900.031.246411
TTATACA22000.030.0204562
CGTCTTC4750.029.637
GATTACG751.4551915E-1129.61
AACAACG250.00549597429.5999987
TATACAC23000.029.117393
GGTATCA5050.028.2079221
CCGTCTT16050.025.7040537
GCCGTCT16450.025.19148836
GTATCAA15250.024.9901641
AAGTACG502.7017292E-422.19999935
TGCCGTC19950.020.7719335
ATGCCGT20800.019.83413534
AACGATT701.21897676E-418.522
CGAAACG500.00703419918.49999829
CCGACGT500.00703419918.49999813
TACACAT38900.017.9768645
TATGCCG23200.017.9418133
TTACGGG1258.572897E-917.7600023
GTCAACC1156.402115E-817.69565215
Produced by FastQC (version 0.11.5)