##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631058.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987566
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49483983855256	31.0	31.0	34.0	28.0	34.0
2	31.50546191343161	31.0	31.0	34.0	30.0	34.0
3	30.96086540038843	31.0	31.0	34.0	27.0	34.0
4	35.082490689229886	37.0	35.0	37.0	32.0	37.0
5	35.31060405076724	37.0	35.0	37.0	33.0	37.0
6	35.505396095045796	37.0	35.0	37.0	33.0	37.0
7	35.52193169874216	37.0	35.0	37.0	33.0	37.0
8	35.543972757263816	37.0	35.0	37.0	33.0	37.0
9	37.31899741384373	39.0	37.0	39.0	34.0	39.0
10	37.15667509817065	39.0	37.0	39.0	33.0	39.0
11	37.2143644070371	39.0	37.0	39.0	33.0	39.0
12	37.157835526941994	39.0	37.0	39.0	33.0	39.0
13	37.221433301673	39.0	37.0	39.0	34.0	39.0
14	38.375926267206445	40.0	38.0	41.0	34.0	41.0
15	38.37851039829237	40.0	38.0	41.0	34.0	41.0
16	38.37396791708098	40.0	38.0	41.0	34.0	41.0
17	38.35880842394331	40.0	38.0	41.0	34.0	41.0
18	38.3894524517855	40.0	38.0	41.0	34.0	41.0
19	38.42364965987083	40.0	38.0	41.0	34.0	41.0
20	38.42467743928001	40.0	38.0	41.0	34.0	41.0
21	38.371074945877034	40.0	38.0	41.0	34.0	41.0
22	38.31201863976686	40.0	38.0	41.0	34.0	41.0
23	38.30450623046966	40.0	38.0	41.0	34.0	41.0
24	38.271894739187054	40.0	38.0	41.0	34.0	41.0
25	38.26280673899264	40.0	38.0	41.0	34.0	41.0
26	38.15658396502107	40.0	38.0	41.0	33.0	41.0
27	38.09449495021092	40.0	37.0	41.0	33.0	41.0
28	38.07739229580605	40.0	37.0	41.0	33.0	41.0
29	38.00407061401466	40.0	37.0	41.0	33.0	41.0
30	37.9519859938475	40.0	37.0	41.0	33.0	41.0
31	37.905059509946675	40.0	37.0	41.0	33.0	41.0
32	37.82179013858314	40.0	37.0	41.0	33.0	41.0
33	37.81763446696221	40.0	37.0	41.0	33.0	41.0
34	37.75760708651371	40.0	37.0	41.0	33.0	41.0
35	37.75796959393094	40.0	37.0	41.0	33.0	41.0
36	37.73645812026741	40.0	37.0	41.0	33.0	41.0
37	37.67559636520496	40.0	37.0	41.0	32.0	41.0
38	37.634539868727764	40.0	37.0	41.0	32.0	41.0
39	37.555954741252734	40.0	37.0	41.0	32.0	41.0
40	37.47751340163594	40.0	37.0	41.0	32.0	41.0
41	37.43348495189182	40.0	37.0	41.0	32.0	41.0
42	37.389431187383934	39.0	36.0	41.0	32.0	41.0
43	36.75047541126365	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	18.0
11	9.0
12	12.0
13	10.0
14	7.0
15	9.0
16	11.0
17	9.0
18	19.0
19	40.0
20	74.0
21	132.0
22	298.0
23	563.0
24	1050.0
25	1818.0
26	3151.0
27	4958.0
28	7495.0
29	10959.0
30	15189.0
31	20521.0
32	26747.0
33	35901.0
34	47448.0
35	64514.0
36	89606.0
37	133722.0
38	236226.0
39	287037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.01325683549251	22.70815317659782	15.149468491219828	24.12912149668984
2	13.538133147556719	22.308483686153636	41.42224418418617	22.731138982103474
3	14.42384610243771	28.783291445837545	34.64507688600053	22.147785565724217
4	11.48976372212085	19.442244872747747	38.73776537466863	30.330226030462775
5	11.909786282638326	38.82849348803017	36.45447494142164	12.807245287909872
6	26.962653635301336	40.96829984021321	17.92730814953127	14.14173837495418
7	23.799219495203356	33.70488655948058	23.462938173246144	19.03295577206992
8	24.10340169669673	36.000328079338495	22.38493427274734	17.51133595121744
9	23.928527308554568	15.42276668091044	19.93314877182892	40.715557238706076
10	13.81710184433243	29.39337725276083	34.36317167662718	22.42634922627956
11	30.74042646263642	23.726110457427655	25.804756340335732	19.728706739600188
12	20.24917828276794	26.844180540844864	29.858358833738706	23.04828234264849
13	28.06738992634416	21.362521593493497	27.65141772803033	22.91867075213201
14	21.221163952586462	22.625930823863925	27.104517571483832	29.048387652065788
15	23.255863405585046	29.386997932290093	23.494733516544716	23.862405145580144
16	23.90938934714237	28.440529544354504	24.081934777017437	23.568146331485693
17	20.723273178704005	30.768880257116994	25.56031698134606	22.947529582832946
18	23.24553498196576	26.67760939522017	26.365123951209334	23.71173167160473
19	23.591435914156623	27.42206596824921	26.621511878699756	22.364986238894414
20	23.926299609342568	26.303254668548735	26.346998580348046	23.423447141760654
21	24.126083725037113	27.00710636048629	25.9064204316471	22.960389482829502
22	21.28303323524706	31.21036973731376	26.326442992164573	21.180154035274604
23	23.513972737012008	26.250498700846325	26.617866552716478	23.617662009425192
24	22.903178116703085	28.14394177199296	25.95806254974351	22.994817561560442
25	22.623095570321375	27.84573385474996	26.583539733040627	22.947630841888035
26	23.459900401593412	26.853901410133602	27.11768124864566	22.568516939627326
27	22.356885514487132	29.73613915424387	26.294242612645636	21.612732718623363
28	22.70926702620382	26.354694268534963	27.911349722448932	23.02468898281229
29	21.352699465149673	27.888667694108545	26.532910205495124	24.225722635246658
30	24.126286243147295	26.69127936765745	27.490820866656	21.691613522539253
31	23.664747470042506	26.27135806619507	26.95273024790242	23.111164215860004
32	21.493854587946526	28.6319091584765	27.356348841495148	22.517887412081823
33	22.4180459837621	26.20857745203865	29.99070441874265	21.382672145456606
34	21.120411192770913	28.025266159426305	26.962349858136065	23.891972789666717
35	21.02613901248119	26.59913362752464	29.883167302236004	22.491560057758164
36	22.324178839692742	26.511342026760744	27.795408104369734	23.369071029176784
37	22.059285151574677	27.261772884040152	28.121158484597487	22.55778347978768
38	22.04693154685358	26.100027744981098	28.680209727754903	23.17283098041042
39	23.255762146529953	26.583235955875356	27.253773418687967	22.907228478906728
40	20.955662710137855	25.91158464345674	31.27871959950019	21.85403304690522
41	21.86132369887177	26.827877832975215	27.067659275430707	24.24313919272231
42	22.516571044365644	25.884953511967808	29.329179011833133	22.269296431833414
43	22.412881771952456	25.373088988482795	29.696243086538015	22.517786153026734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17.0
1	18.5
2	20.0
3	100.5
4	181.0
5	181.0
6	211.0
7	241.0
8	248.5
9	256.0
10	408.5
11	561.0
12	561.0
13	858.5
14	1156.0
15	2709.0
16	4262.0
17	5033.0
18	5804.0
19	5804.0
20	6489.5
21	7175.0
22	9386.5
23	11598.0
24	15164.0
25	18730.0
26	18730.0
27	23303.0
28	27876.0
29	33755.0
30	39634.0
31	43708.5
32	47783.0
33	47783.0
34	52253.0
35	56723.0
36	60286.5
37	63850.0
38	67388.0
39	70926.0
40	70926.0
41	72763.5
42	74601.0
43	72537.5
44	70474.0
45	70163.0
46	69852.0
47	69852.0
48	67239.0
49	64626.0
50	63137.0
51	61648.0
52	61831.5
53	62015.0
54	62015.0
55	70563.5
56	79112.0
57	58415.5
58	37719.0
59	41733.0
60	45747.0
61	45747.0
62	37643.0
63	29539.0
64	21659.5
65	13780.0
66	11320.0
67	8860.0
68	8860.0
69	7006.5
70	5153.0
71	4384.0
72	3615.0
73	2793.0
74	1971.0
75	1971.0
76	1538.0
77	1105.0
78	828.0
79	551.0
80	387.0
81	223.0
82	223.0
83	159.0
84	95.0
85	74.5
86	54.0
87	37.0
88	20.0
89	20.0
90	15.5
91	11.0
92	6.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	987566.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.238224455577395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.34086697867045	43.83681558077298
2	10.715718022000965	11.409716025159264
3	2.820965471406357	4.505495788444959
4	1.1457152576405318	2.439833841937853
5	0.6083538448972041	1.6193839271525436
6	0.3928743725598894	1.2549560417512513
7	0.26760248066199355	0.9972676651246767
8	0.1967979025227246	0.8381736725513323
9	0.15729962264538444	0.7536917355455341
>10	1.1184200249604843	11.862303271435051
>50	0.14397742607047603	5.378074851978239
>100	0.08664773163140975	7.8850381378194
>500	0.0028565185993121786	1.0666609121589172
>1k	0.0011426074397248714	1.4510076014464806
>5k	3.8086914657495713E-4	1.706393861091791
>10k+	3.8086914657495713E-4	2.9951870856297775
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT	16525	1.6733058853788	No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG	13018	1.3181903791746576	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	9856	0.9980092469769108	Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	6975	0.7062819092597356	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	3562	0.3606847542341474	Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	3028	0.30661241881555257	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2542	0.25740051804132585	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2242	0.2270228015140254	No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG	1784	0.1806461542823467	No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA	1154	0.11685294957501574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0125905509100152E-4	0.0
5	0.0	0.0	0.0	1.0125905509100152E-4	0.0
6	0.0	0.0	0.0	1.0125905509100152E-4	0.0
7	0.0	0.0	0.0	1.0125905509100152E-4	0.0
8	0.0	0.0	0.0	1.0125905509100152E-4	0.0
9	0.0	0.0	0.0	1.0125905509100152E-4	0.0
10	0.0	0.0	0.0	3.037771652730045E-4	0.0
11	0.0	1.0125905509100152E-4	0.0	3.037771652730045E-4	0.0
12	0.0	1.0125905509100152E-4	0.0	3.037771652730045E-4	0.0
13	0.0	2.0251811018200304E-4	0.0	4.0503622036400607E-4	0.0
14	0.0	2.0251811018200304E-4	0.0	4.0503622036400607E-4	0.0
15	0.0	2.0251811018200304E-4	0.0	4.0503622036400607E-4	0.0
16	0.0	2.0251811018200304E-4	0.0	4.0503622036400607E-4	0.0
17	0.0	2.0251811018200304E-4	0.0	5.062952754550076E-4	0.0
18	0.0	2.0251811018200304E-4	0.0	6.07554330546009E-4	0.0
19	0.0	2.0251811018200304E-4	0.0	6.07554330546009E-4	0.0
20	0.0	2.0251811018200304E-4	0.0	9.113314958190136E-4	0.0
21	0.0	2.0251811018200304E-4	0.0	0.001215108661092018	0.0
22	0.0	2.0251811018200304E-4	0.0	0.0016201448814560243	0.0
23	0.0	2.0251811018200304E-4	0.0	0.0018226629916380272	0.0
24	0.0	2.0251811018200304E-4	0.0	0.0021264401569110318	0.0
25	1.0125905509100152E-4	2.0251811018200304E-4	0.0	0.002227699212002033	0.0
26	1.0125905509100152E-4	2.0251811018200304E-4	0.0	0.002531476377275038	0.0
27	1.0125905509100152E-4	2.0251811018200304E-4	0.0	0.0031390307078210467	0.0
28	1.0125905509100152E-4	2.0251811018200304E-4	0.0	0.007290651966552109	0.0
29	1.0125905509100152E-4	2.0251811018200304E-4	0.0	0.01539137637383223	0.0
30	2.0251811018200304E-4	2.0251811018200304E-4	0.0	0.027542462984752412	0.0
31	2.0251811018200304E-4	2.0251811018200304E-4	0.0	0.06379320470733095	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2090	0.0	31.24641	1
TTATACA	2200	0.0	30.020456	2
CGTCTTC	475	0.0	29.6	37
GATTACG	75	1.4551915E-11	29.6	1
AACAACG	25	0.005495974	29.599998	7
TATACAC	2300	0.0	29.11739	3
GGTATCA	505	0.0	28.207922	1
CCGTCTT	1605	0.0	25.70405	37
GCCGTCT	1645	0.0	25.191488	36
GTATCAA	1525	0.0	24.990164	1
AAGTACG	50	2.7017292E-4	22.199999	35
TGCCGTC	1995	0.0	20.77193	35
ATGCCGT	2080	0.0	19.834135	34
AACGATT	70	1.21897676E-4	18.5	22
CGAAACG	50	0.007034199	18.499998	29
CCGACGT	50	0.007034199	18.499998	13
TACACAT	3890	0.0	17.976864	5
TATGCCG	2320	0.0	17.94181	33
TTACGGG	125	8.572897E-9	17.760002	3
GTCAACC	115	6.402115E-8	17.695652	15
>>END_MODULE