FastQCFastQC Report
Fri 10 Feb 2017
ERR1631057.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631057.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113889
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT85837.536285330453336RNA PCR Primer, Index 26 (100% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG58325.1207754919263495No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC37203.266338276743145No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC31582.77287534353625RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC25822.267119739395376No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG9760.8569747736831477No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT7880.6919017639982791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7400.6497554636532062No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT6590.5786335818208959RNA PCR Primer, Index 26 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6160.5408775210951013No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT4850.4258532430700067No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA4760.4179508117553056No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT3240.2844875273292416RNA PCR Primer, Index 26 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2880.252877802070437No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG2810.24673146660344725No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG2570.2256583164309108No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT2020.1773656806188482No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG1960.17209739307571406No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1620.1422437636646208No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA1510.1325852365022083No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA1430.1255608531113628No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC1430.1255608531113628No Hit
TCGCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT1300.11414623010123892RNA PCR Primer, Index 26 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC3700.033.037
CTTATAC1350.030.148151
GGCGGCC250.00547582629.59999837
CATAGGT405.8939622E-527.7516
GGTATCA604.251342E-827.7499981
GTATCAA2350.027.553191
ATACACA8500.026.9882341
CCGTCTT12400.026.25806437
TACACAT8750.026.2171442
TCCATAG508.997524E-625.89999814
GCCGTCT12750.025.68235236
CCATAGG451.3130107E-424.66666815
TTATACA1750.023.2571412
TGGCAGT450.003804989920.55555711
TACTAAC450.003804989920.5555577
AATCCAT555.1040825E-420.18181812
TGCCGTC16600.019.94879535
ACGGGAT759.146137E-619.73333415
TAACTGA759.146137E-619.7333346
ATGCCGT17500.018.92285734
Produced by FastQC (version 0.11.5)